Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1969; 22(02): 234-239DOI: 10.1055/s-0038-1651438 Originalarbeiten-Original Articles-Travaux Originaux Schattauer GmbH The Purification of Fibrinogen Degradation Products by Pevikon Block Electrophoresis Authors Author Affiliations V. J Marder 1 1 Dr. Theodore H. Mendell Research Laboratory, Temple University School of Medicine. Philadelphia, Pa. 19140, USA H. L James 1 Dr. Theodore H. Mendell Research Laboratory, Temple University School of Medicine. Philadelphia, Pa. 19140, USA S Sherry 1 Dr. Theodore H. Mendell Research Laboratory, Temple University School of Medicine. Philadelphia, Pa. 19140, USA Recommend Article Abstract Buy Article(opens in new window) Summary The use of Pevikon block electrophoresis for the purification of bulk quantities of the major end-products of plasmin digestion of fibrinogen has been described. Greater than 50% yields of Fragments D and E were obtained, each free of contamination with the other. Full Text References References 1 Nussenzweig V, Seligmann M. Analyse, par des méthodes immunochimiques, de la dégradation par la plasmine du fibrinogène humain et de la fibrine, á différents stades. Rev. Hémat. 15: 451 1960; 2 Nussenzweig V, Seligmann M, Pelmont J, Grabar P. Les produits de dégradation du fìbrinogène humain par la plasmine. I. Séparation et propriétés physico-chimiques. Ann. Inst. Pasteur 100: 377 1961; 3 Marder V. J, Shulman N. R, Carroll W. R. The importance of intermediate degradation products of fibrinogen in fibrinolytic hemorrhage. Trans. Assoc. Amer. Phys. 80: 156 1967; 4 Marder V. J, Shidman N. R, Carroll W. R. High molecular weight derivatives of human fibrinogen produced by plasmin. I. Physicochemical and immunological characterization. J. biol. Chem. 244: 2111 1969; 5 Müller-Eberhard H. J, Kunkel H. G. The carbohydrate of λ-globulin and myeloma proteins. J. exp. Med. 104: 253 1956; 6 Larrieu M.-J, Marder V. J, Inceman S. Effects of fibrinogen degradation products on platelets and coagulation. In: Diffuse Intravascular Clotting. Trans. Int. Comm. on Haemostasis and Thrombosis, 1965. Eds. Koller F, Streuli F. 215 F. K. Schattauer Verlag; Stuttgart: 1966 7 Niléhn J.-E. Split products of fibrinogen after prolonged interaction with plasmin. Thrombos. Diathes. haemorrh. (Stuttg.) 18: 89 1967; 8 Jamieson G. A, Gajfney Jr. P. J. Nature of the high molecular weight fraction of fibrinolytic digests of human fibrinogen. Biochim. biophys. Acta (Amst.) 154: 96 1968; 9 Fletcher A. P, Alkjaersig N, Fischer S, Sherry S. The proteolysis of fibrinogen by plasmin : The indentification of thrombinclottable fibrinogen derivatives which polymerize abnormally. J. Lab. clin. Med. 68: 780 1966; 10 Johnson A. J, Kline D. L, Alkjaersig N. Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968. Thrombos. Diathes. haemorrh. (Stuttg.) 21: 259 1969; 11 Davis B. J. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121: 404 1964; 12 Salafsky B, Loomis T. A. Paper electrophoresis of fibrinogen and its products. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 155 1963; 13 Scheidegger J. J. Une micro-méthode de l’immuno-électrophorèse. Int. Arch. Allergy 7: 103 1955; 14 Marder V. J. Immunologic structure of fibrinogen and its split products of plasmin degradation. Theoretical and clinical considerations. In: Fibrinogen. Ed. Laki Koloman. 339 Marcel Dekker, Inc.; New York: 1968
