Dependence of Platelet Adenyl Cyclase System on Oxidative Phosphorylation

Shuichi Hashimoto; Sachiko Shibata; Bokro Kobayashi

doi:10.1055/s-0038-1651444

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1975; 34(01): 042-049
DOI: 10.1055/s-0038-1651444

Original Article

Schattauer GmbH

Dependence of Platelet Adenyl Cyclase System on Oxidative Phosphorylation

Shuichi Hashimoto

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, Kitasato University School of Pharmaceutical Sciences, Tokyo, Japan

,

Sachiko Shibata

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, Kitasato University School of Pharmaceutical Sciences, Tokyo, Japan

,

Bokro Kobayashi

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, Kitasato University School of Pharmaceutical Sciences, Tokyo, Japan

› Author Affiliations

Abstract

Summary

The radioactive adenosine 3′,5′-monophosphate (cyclic AMP) level derived from 8-¹⁴C adenine in intact rabbit platelets decreased in the presence of mitochondrial inhibitor (potassium cyanide) or uncoupler (sodium azide), and markedly increased by the addition of NaF, monoiodoacetic acid (MIA), or 2-deoxy-D-glucose. The stimulative effect of the glycolytic inhibitors was distinctly enhanced by the simultaneous addition of sodium succinate. MIA did neither directly stimulate the adenyl cyclase activity nor inhibit the phosphodiesterase activity. These results suggest that cyclic AMP synthesis in platelets is closely linked to mitochondrial oxidative phosphorylation.

Full Text

References

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