Single Step Purification of Platelet Factor XIII Using an Immobilized Factor XIII A-Subunit Monoclonal Antibody

Daniela Lukacova; Guy L Reed

doi:10.1055/s-0038-1651620

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1993; 69(04): 397-400
DOI: 10.1055/s-0038-1651620

Original Article

Platelets

Schattauer GmbH Stuttgart

Single Step Purification of Platelet Factor XIII Using an Immobilized Factor XIII A-Subunit Monoclonal Antibody

Daniela Lukacova

¹The Massachusetts General Hospital, Boston, Massachusetts, USA

,

Guy L Reed

²Harvard Medical School, Boston, Massachusetts, USA

› Author Affiliations

Abstract

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Summary

To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.

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References

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