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Thromb Haemost 1993; 69(04): 397-400
DOI: 10.1055/s-0038-1651620
Original Article
Platelets
Schattauer GmbH Stuttgart

Single Step Purification of Platelet Factor XIII Using an Immobilized Factor XIII A-Subunit Monoclonal Antibody

Daniela Lukacova
1   The Massachusetts General Hospital, Boston, Massachusetts, USA
,
Guy L Reed
2   Harvard Medical School, Boston, Massachusetts, USA
› Author Affiliations
Further Information

Publication History

Received 04 August 1992

Accepted after revision 14 December 1992

Publication Date:
05 July 2018 (online)

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Summary

To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.

 

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