PDF herunterladen
Thromb Haemost 1981; 46(02): 489-495
DOI: 10.1055/s-0038-1653394
Original Article
Schattauer GmbH Stuttgart

Generation of Tissue Factor by Patient Monocytes: Correlation to Thromboembolic Complications

Carol L Miller
The Departments of Surgery and Microbiology-Immunology, University of California, San Francisco, U.S.A
,
Charles Graziano
The Departments of Surgery and Microbiology-Immunology, University of California, San Francisco, U.S.A
,
Robert C Lim
The Departments of Surgery and Microbiology-Immunology, University of California, San Francisco, U.S.A
,
Milly Chin
The Departments of Surgery and Microbiology-Immunology, University of California, San Francisco, U.S.A
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 05. Januar 1981

Accepted 22. Mai 1981

Publikationsdatum:
05. Juli 2018 (online)

Auch verfügbar auf
  als PDF herunterladen  Lizenzen und Reprints

Summary

Thromboembolic complications are often a common pathological consequence of severe soft tissue trauma. Recent demonstration that monocytes (MØ) produce tissue factor (TF) has led to the suggestion that these TF producing MØ might play a role in coagulopathy. We have previously demonstrated that trauma patients with splenectomy develop aberrant monocyte function and this patient group is also known to be at high risk of hypercoagulability episodes. This paper is an intial report on the use of MØ TF as an indicator of and/or a correlate to clotting episodes. Monocytes isolated from the Ficoll-Hypaque purified mononuclear cells of 46 normal individuals, 17 trauma patients and 6 surgical controls were assayed at 3 day post-injury intervals for their levels of TF activity. Changes in monocyte TF activity were correlated to increases in the fractional catabolic rate (FCR) of 125I-fibrinogen. Trauma patients were retrospectively divided into those whose FCR was elevated to a level indicative of coagulopathy and those whose FCR levels were not associated with coagulation abnormalites. All trauma patients who exhibited significantly increased FCR experienced thromboembolic episodes and had monocytes whose TF activity was increased an average of 300% (X = 47 units vs X = 12 units) over surgical controls. These increases in monocyte TF activity occrured at 6-13 days post injury and preceded clinical manifestation of coagulopathy by 4-6 days. The increased monocyte TF activity demonstrated in this study was significantly correlated to detection of pathologically increased FCR (Rs = 0.850) and compared to other indices of hypercoagulability.

Keywords

Monocyte - Procoagulant tissue factor (TF) - Coagulopathy - Trauma
 

  • References

  • 1 Edwards RL, Rickies RF. The role of human T cells (and T cell products) for monocyte tissue factor generation. J Immunol 1980; 125: 606-609
    PubMedSuche in Google Scholar
  • 2 Osterud J, Berre A, Otnaess AB, Bjorklid E, Prydz H. Activation of the coagulation factor VII by tissue thromboplastin and calcium. Biochemistry 1972; 2: 2853-2857
    PubMedSuche in Google Scholar
  • 3 Rivers RP A, Hathaway WE, Weston WL. The endotoxin-induced coagulant activity of human monocytes. Br J Haematol 1975; 31: 311-316
    CrossrefPubMedSuche in Google Scholar
  • 4 Rickies FR, Levin J, Rosenthal DI, Atkins E. Functional interaction of concanvalin A and bacterial endotoxin (lipopolysaccharide): Effects on the measurement of endogenous pyrogen release, human mononuclear cell tissue factor activation, lymphocyte DNA synthesis and gelation of Limulus amebocyte lysate. J Lab Clin Med 1979; 93: 128-145
    PubMedSuche in Google Scholar
  • 5 Levy GA, Edginton TS. Lymphocyte cooperation is required for amplification of macrophage procoagulant activity. J Exp Med 1980; 151: 1232-1244
    CrossrefPubMedSuche in Google Scholar
  • 6 Prydz H, Allison AC. Tissue thromboplastin activity of isolated human monocytes. Thromb Haemostas 1978; 39: 582-591
    PubMedSuche in Google Scholar
  • 7 Muhlfelder TW, Neimetz J, Kreutzer D, Beebe D, Ward PA, Rosenfeld RI. C5 chemotactic fragment induces leukocyte production of tissue factor activity. J Clin Invest 1979; 63: 147-150
    CrossrefPubMedSuche in Google Scholar
  • 8 Edwards RL, Rickles FR, Bobrove AM. Mononuclear cell tissue factor: Cell of origin and requirements for activation. Blood 1979; 54: 359-370
    PubMedSuche in Google Scholar
  • 9 Hasegawa H, Nagata H, Murao M. Studies on the tissue thrombopla-stin during the coagulation-fibrinolytic process – ultrastructural changes. Thromb Haemostas 1977; 37: 541-548
    PubMedSuche in Google Scholar
  • 10 Stewart GF. The intravascular generation of fibrinogen derivatives and the blood vessel wall in venous thrombosis and disseminated intravascular coagulation. Thromb Haemostas 1977; 38: 831-849
    PubMedSuche in Google Scholar
  • 11 Saldeen T. Trends in microvascular research. The microembolism syndrome Microvasc Res 1976; 11: 227-259
    CrossrefPubMedSuche in Google Scholar
  • 12 Rodvien R, Salzman EW. Thrombotic and hemorrhagic problems in surgery. Thromb Haemostas 1978; 39: 254-262
    PubMedSuche in Google Scholar
  • 13 Robson EB, Murawski GF, Bettigole RE. Use of 125I-fibrinogen kinetic data to detect disseminated intravascular coagulation and deposition of fibrin in patients with metastatic cancer. Thromb Haemostas 1977; 37: 484-508
    PubMedSuche in Google Scholar
  • 14 Jones RL, Peterson CM. Reduced fibrinogen survival in diabetes mellitus. A reversible phenomenon J Clin Invest 1979; 63: 485-493
    CrossrefPubMedSuche in Google Scholar
  • 15 Tomgren S, Forsberg KI, Rieger A. Fibrinogen catabolism in patients with postoperative infectious complications. Scand J Infect Dis 1980; 12: 55-60
    CrossrefPubMedSuche in Google Scholar
  • 16 Effeney DJ, McIntyre KE, Blaisdell FW, Graziano CJ. Fibrinogen kinetics in major human burns. Surg Forum 1975; 29: 56-59
    PubMedSuche in Google Scholar
  • 17 Blaisdell FW, Graziano CJ. Assessment of clotting by the determination of fibrinogen catabolism. Am J Surg 1976; 135: 436-443
    PubMedSuche in Google Scholar
  • 18 Miller CL, Baker C. Changes in lymphocyte activity after thermal injury. The role of suppressor cells J Clin Invest 1979; 63: 202-210
    CrossrefPubMedSuche in Google Scholar
  • 19 Ackerman SK, Douglas SD. Purification of human monocytes on microexudated coated surfaces. J Immunol 1978; 120: 1372-1374
    PubMedSuche in Google Scholar
  • 20 Yam LT, Li CY, Crosby WH. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 1971; 55: 283-290
    CrossrefPubMedSuche in Google Scholar
  • 21 Li CY, Lam DW, Yam LT. Esterases in human leukocytes. J Histochem Cytochem 1973; 21: 1-12
    CrossrefPubMedSuche in Google Scholar
  • 22 Peade DC. Histological techniques for electronmicroscopy. Academic Press; New York: 1964: 52-56
    Suche in Google Scholar
  • 23 Rickies RF, Hardin JA, Pitlick FA. Tissue factor activity in lymphocyte cultures from normal individuals and patients with Hemophilia A. J Clin Invest 1973; 52: 1427-1434
    CrossrefPubMedSuche in Google Scholar
  • 24 Collen D, Tytgat GN, Claeys H, Piessens R. MetabolismMetabolism and distribution of fibrinogen. I.. fibrinogen turnover in physiological conditions in humans Br J Haematol 1972; 22: 681-684
    CrossrefPubMedSuche in Google Scholar
  • 25 Draper N, Smith H. Applied Regression Analysis. Wiley; New York: 1966
    Suche in Google Scholar
  • 26 Kakkar VV. Deep venous thrombosis detection and prevention. Circulation 1975; 51: 8-15
    CrossrefPubMedSuche in Google Scholar
  • 27 Kidder WR, Logan LJ, Rapaport SI. The plasma protamine paracoagulation test: clinical and laboratory evaluation. Am J Clin Pathol 1972; 58: 675-686
    CrossrefPubMedSuche in Google Scholar
  • 28 Pitcher PM. The use of rapid latex screening test for the detection of fibrinogen degradation products (FDP) in urine. In: Abstracts of the Third Congress of the International Society of Thrombosis and Haemostasis
    PubMed
  • 29 Zar JH. Biostatistical Analysis. Prentice-Hall, Inc.,; Englewood Cliff, New Jersey: 1974
    Suche in Google Scholar
  • 30 McLoughin GA, Grindlinger GA, Manny J, Valeri CR, Lipinski Mannick LA, Hechtman HB. Intrapulmonary clotting and fibrinolysis during abdominal aortic aneurysm surgery. Ann Surg 1979; 190: 623-630
    CrossrefPubMedSuche in Google Scholar
  • 31 Van Ginkel CJ W, Van Aken WG, Oh JI H, Vreeken J. Is leukocyte procoagulant activity generated by neutrophils or monocytes. Thromb Res 1978; 12: 569-571
    CrossrefPubMedSuche in Google Scholar
  • 32 Fauve RM. Inflammation and natural immunity. In: Fougereau M, Dausset J. eds Progress in Immunology. p 134-143 Academic Press; New York: 1980
    Suche in Google Scholar
  • 33 Gopal V, Bisno AL. Fulminant pneumococcal infections in “normal” asplenic hosts. Arch Intern Med 1977; 137: 1526-1530
    CrossrefPubMedSuche in Google Scholar
  • 34 Miller CL, Baker C. Development of an inhibitory macrophage after splenectomy. Transplant Proc 1979; 11: 1460
    PubMedSuche in Google Scholar
  • 35 Lerner RG, Goldstein R, Cummings G. Endotoxin induced disseminated intravascular clotting: Evidence that it is mediated by neutrophil production of tissue factor. Thromb Res 1977; 11: 253-261
    CrossrefPubMedSuche in Google Scholar