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DOI: 10.1055/s-0038-1653399
Long-Term Tolerance and Recovery of β-Propiolactone/Ultraviolet (βPL/UV) Treated PPSB in Chimpanzees
Publikationsverlauf
Received 16. März 1981
Accepted 11. Mai 1981
Publikationsdatum:
05. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198102/lookinside/thumbnails/10-1055-s-0038-1653399-1.jpg)
Summary
Recent experiments have shown that a preparation of PPSB (factor IX concentrate) derived from βPL/UV treated plasma was not infectious in chimpanzees with respect to hepatitis B and non-A, non-B. To answer the question whether the βPL/UV treatment influences the tolerance and efficacy of the PPSB concentrate, long-term application of PPSB-Biotest was carried out in chimpanzees. After 10 applications of 25 U factor IX/kg at weekly intervals, no signs of intolerance were observed by measurement of blood pressure during i. v. application and by means of skin-testing. Determination of coagulation factor activity during the application period revealed the same factor IX recovery at the beginning and at the end of the study.
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References
- 1 Stephan W, Kotitschke R. Prothrombinkomplex-Konzentrat aus kalt-sterilisiertem Plasma. Forschungsergebnisse der Transfusionsmedizin und Immunhämatologie 1977; 4: 72-75
- 2 Stephan W. Hepatitis-free and stable human serum for intravenous therapy. Vox Sang 1971; 20: 442-457
- 3 Bauer HW, Mellin HE, Grabs GH. Über die Bedeutung der postoperativen Zufuhr von Plasmaproteinen. Infusionstherapie 1980; 7: 301-304
- 4 Prince AM, Stephan W, Brotman B, Van den Ende MC. Evaluation of the effect of betapropiolactone/ultraviolet irradiation (BPL/UV) treatment of source plasma on hepatitis transmission by factor IX complex in chimpanzees. Thromb Haemostas 1980; 44: 138-142
- 5 Stephan W, Prince AM, Berthold H. Inaktivierung von Hepatitis B-Viren in Human-Plasma und Serum durch β-Propiolacton (βPL)/UV-Behandlung. Forschungsergebnisse der Transfusionsmedizin und Immunhämatologie 1980; 7: 559-570
- 6 Prince AM, Stephan W, Brotman B. β-propiolactone/ultraviolet irradiation: Quantitative studies on effectiveness for inactivation of hepatitis B virus. Abstract. Symposium on Viral Hepatitis. New York, N. Y.: 28.03.1981
- 7 Stephan W. Über die Hydrolyse von β-Propiolacton in Seren und Plasmen. Z Klin Chem Klin Biochem 1969; 7: 518-520
- 8 Stephan W, Haug E. Unterschiedliche Wirksamkeit von β-Propiolacton bei der Sterilisation von Esterase-aktiven und -inaktiven Seren und Plasmen. Zentralbl Bakteriol 1970; 215: 381-385
- 9 Sonneborn HH, Stephan W. Untersuchungen zur Verträglichkeit von β-Propiolacton-behandeltem und UV-bestrahltem Rattenserum. Infusionstherapie 1980; 7: 145-147
- 10 Kornhuber B. Intravenöse Immunglobulin-Langzeittherapie bei Kindern. Monatsschr Kinderheilk 1979; 127: 20-22
- 11 Kotitschke R, Stephan W. Fractionating citrate’stabilized plasma. US Patent Ser No 75867
- 12 Ovary Z. Passive cutaneous anaphylaxis. In: Ackroyd, Immunological Methods 1964. Blackwell: London-Oxford-Edinburgh; 1964: 259-283
- 13 Sonnebom HH, de Week AL, Toffler O. Vergleichende Studie über die Immunogenität von Plasmaersatzlösungen auf Gelatinebasis im Tierexperiment. Infusionstherapie 1978; 5: 41-49
- 14 Ouchterlony Ö. Diffusion in gel methods for immunological analysis. Prog Allergy 1958; 5: 1-78
- 15 Davies OL, Goldsmith PL. Statistical methods in research and production. Chapter 6: Analysis of variance. In: Statistical Methods in Research and Production, with special reference to the chemical industry, published for ICI. Longman Group Ltd.; London: 1972: 121-177