Factor VIII : C and Factor VIII R: Ag in Argentine Hemorrhagic Fever

Felisa C Molinas; Julio I Maiztegui

doi:10.1055/s-0038-1653402

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1981; 46(02): 525-527
DOI: 10.1055/s-0038-1653402

Original Article

Schattauer GmbH Stuttgart

Factor VIII : C and Factor VIII R: Ag in Argentine Hemorrhagic Fever

Felisa C Molinas

Sección Hematología, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires and Centro de Estudios sobre Fiebre hemorrágica argentina, Pergamino, Buenos Aires, Argentina

,

Julio I Maiztegui

Sección Hematología, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires and Centro de Estudios sobre Fiebre hemorrágica argentina, Pergamino, Buenos Aires, Argentina

› Author Affiliations

Abstract

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Summary

Factor VIII procoagulant activity (F VIII: C) and factor VIII related antigen (F VIII R: Ag) were investigated in 35 patients with Argentine hemorrhagic fever. Since the results obtained in the three clinical forms of the disease were not significantly different, they were tabulated altogether. F VIII: C was low in early stages of the disease but increased progressively in later days (days 5–6:0.54 ± 0.10 I.U./ml; days 13–14:0.95 ± 0.13 I.U./ml). In contrst, the levels of F VIII R: Ag were high all along the disease and they returned to normal values during the convalescence period (days 5–6; 2.58 ± 0.54 I.U./ml; day 30: 1.30 ± 0.14 I.U./ml). The levels of F VIII R: Ag were similar in samples drawn before (11 cases) or after (10 cases) the treatment with immune plasma infusion. Plasma samples from 12 patients were studied by two-dimensional immunelectrophoresis. The only abnormality found was increased height of the immune precipitation arc.

Keywords

F VIII: C–F VIIIR: Ag - Argentine hemorrhagic fever

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References

References
1 Schwartz ER, Mandó OG, Maiztegui JI, Vilches AM. Sintomas y signos iniciales de valor diagnóstico en la Fiebre hemorrágica argentina. Medicina (B Aires) 1970; 30 Suppl (Suppl. 01) 8-14
2 de Braceo MM E, Rimoldi MT, Cossio PM, Rabinovich A, Maiztegui JI, Carballal G, Arana RM. Argentine hemorrhagic fever. Alterations of the complement system and anti-Junin-virus humoral response New Engl J Med 1978; 229: 216-221
3 Schwartz ER, Mandó OG, Maiztegui JI, Vilches AM, Otero ER, Berrutti ZC. Alteraciones de la coagulación en la Fiebre hemorrágica argentina. Medicina (B Aires) 1972; 32: 247-259
4 Agrest A, Sánchez-Avalos JC, Arce M, Slepoy A. Fiebre hemorrágica argentina y coagulopatía por consumo. Medicina (B Aires) 1969; 29: 194-201
5 Molinas FC, de Braceo MM E, Maiztegui JI. Coagulation studies in Argentine hemorrhagic fever. J Infect Dis 1981; 143: 1-6
6 Molinas FC, Paz RA, Rimoldi MT, de Braceo MM E. Studies of blood coagulation and pathology in experimental infection of guinea pigs with Junin virus. J Infect Dis 1978; 137: 740-746
7 Maiztegui JI, Fernandez NJ, de Damilano AJ. Reducción de la mortahdad en la Fiebre hemorrágica argentina (FHA) mediante el tratamiento con plasma inmune. Medicina (B Aires) 1978; 38: 743
8 Rapaport S, Schiffman S, Patch MJ, Ware AG. A simple specific one-stage assay for plasma thromboplastin antecedent activity. J Lab Clin Med 1961; 57: 771-780
9 Laurell CB. Electroimmunoassay. Scand J Clin Lab Invest 1972; 29 Suppl (Suppl. 124) 21-37
10 Laurell CB. Antigen-antibody crossed electrophoresis. Anal Biochem 1965; 10: 358-361
11 Schwartz D. Méthodes statistiques à l’usage des médidns et des biologistes. Twelfth edition Paris, medicales Flammarion; 1966. pp 151-166
12 Green J, Ratnoff OD. Elevated antihemophilic factor (AHF, F VIII) procoagulant activity and AHF like antigen in alcoholic cirrhosis of the liver. J Lab Clin Med 1974; 83: 189-197
13 Denson KW. The ratio of F VIII RAG and F VIII biological activity as an index of hypercoagulability and intravascular clotting. Thromb Res 1977; 10: 107-119
14 Molinas FC, Molina MM, Acciaresi MS, Sánchez-Avalos JC. Estudio del F VIII-RAG y F VIII-C en pacientes con inhibidores de la coagulación y con lupus eritematoso sistèmico (LES). (Abstract) Medicina (B Aires) 1979; 39: 717
15 Kopec M, Bykowska K, Łopaciuk S, Jelenska M, Kaczanowska J, Sopata I, Wojtecka E. Effects of neutral proteases from human leukocytes on structure and biological properties of human factor vra. Thromb Haemostas 1980; 43: 211-217
16 Egbring R, Schmidt W, Fuchs G, Havemann K. Demonstration of granulocytic proteases in plasma of patients with acute leukemia and septicemia with coagulation defects. Blood 1977; 49: 219-231
17 Gallardo F. Fiebre hemorrágica argentina. Hallazgos anatomo-patológicos en 10 necropsias Medicina (B Aires) 1970; Suppl (Suppl. 01) 77-84
18 Eisner B, Schwartz E, Mandó OG, Maiztegui JI, Vilches A. Pathology of 12 cases of Argentine hemorrhagic fever. A J Trop Med Hyg 1973; 22: 229-236
19 Kierszenbaum F, Budzko DB, Parodi AS. Alterations in the enzymatic activity of plasma of guinea pigs infected with Junin virus. Arch Gesamte Virusforsch 1970; 30: 217-223
20 Rimoldi MT, de Bracco MM E. In vitro inactivation of complement by a serum factor present in Junin virus infected guinea pigs. Immunology 1980; 39: 159-164