The In Vitro Inhibitory Effect on Thrombin by 2,3-Diphosphoglycerate

D Del Principe; P M Strappini; S Del Vecchio; A Menichelli; G Biancini; E V Cosmi; V Bastianon; C D’Arcangelo

doi:10.1055/s-0038-1653422

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1981; 46(03): 581-583
DOI: 10.1055/s-0038-1653422

Original Article

Schattauer GmbH Stuttgart

The In Vitro Inhibitory Effect on Thrombin by 2,3-Diphosphoglycerate

Authors

D Del Principe

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
P M Strappini

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
S Del Vecchio

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
A Menichelli

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
G Biancini

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
E V Cosmi

^*The Departments of Anesthesiology and Obstetrics and Gynecology, Medical School, University of Rome, Italy
V Bastianon

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
C D’Arcangelo

The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

Abstract

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Summary

Thrombin, incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, α-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, α-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.

Keywords

2,3-Diphosphoglycerate - Thrombin - Blood coagulation

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Referenzen

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