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Thromb Haemost 1981; 46(03): 581-583
DOI: 10.1055/s-0038-1653422
Original Article
Schattauer GmbH Stuttgart

The In Vitro Inhibitory Effect on Thrombin by 2,3-Diphosphoglycerate

D Del Principe
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
P M Strappini
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
S Del Vecchio
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
A Menichelli
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
G Biancini
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
E V Cosmi
*   The Departments of Anesthesiology and Obstetrics and Gynecology, Medical School, University of Rome, Italy
,
V Bastianon
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
,
C D’Arcangelo
The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
› Institutsangaben
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Publikationsverlauf

Received 17. September 1980

Accepted 15. Juni 1981

Publikationsdatum:
05. Juli 2018 (online)

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Summary

Thrombin, incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, α-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, α-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.

Keywords

2,3-Diphosphoglycerate - Thrombin - Blood coagulation
 

  • References

  • 1 Chanutin A, Curnish RR. Effect of organic and inorganic phosphates on oxygen equilibrium of human erythrocytes. Arch Biochem Biophys 1967; 121: 96-102
    CrossrefPubMedSuche in Google Scholar
  • 2 Oski FA, Gottlieb ArlanJ, Delivoria PapadopulusM, Miller WW. Red cell 2,3-DPG levels in subjects with chronic hypoxia. N Engl J Med 1969; 280: 1165-1166
    CrossrefPubMedSuche in Google Scholar
  • 3 Iatridis SG, Iatridis PG, Markidou SG, Ragatz BH. 2,3- Diphosphoglycerate: a physiological inhibitor of platelet aggregation. Science 1975; 187: 259-261
    CrossrefPubMedSuche in Google Scholar
  • 4 Del Principe D, Menichelli A, Damiano AM, Coppola L, Bastianon V. The effect of 2,3-diphosphoglycerate on oxygen consumption burst in thrombin-stimulated platelets. Clin Chim Acta 1977; 75: 325-329
    CrossrefPubMedSuche in Google Scholar
  • 5 Iatridis PG, Iatridis SG, Ragatz BH. The in vivo increase of Factor VIII activity by 2,3-diphosphoglycerate. Thromb Res 1976; 9: 533-540
    CrossrefPubMedSuche in Google Scholar
  • 6 Yip LC, Balis ME. Inhibitory effects of 2,3-DPG on enzymes of purine nucleotide metabolism. Biochem Biophys Res Commun 1975; 63: 722-729
    CrossrefPubMedSuche in Google Scholar
  • 7 Workman EF, Utheg LC, Kingdon HS, Lundblad RL. Chemical modification of thrombin: effect on binding to platelets in “Chemistry and Biology of Thrombin”. Lundblad RL, Fenton II JW, Mann KG. eds Ann Arbor Science; Ann Arbor: 1977. pp 23-42
    Suche in Google Scholar
  • 8 Del PrincipeD, Mancuso G, D’Arcangelo C, Menichelli A, Strappini PM, Del VecchioS. Separation of thrombin forms by analytical isotachophoresis. Thromb Res 1980; 20: 137-142
    CrossrefPubMedSuche in Google Scholar
  • 9 Lundblad RL. A rapid method for the purification of bovine thrombin and the inhibition of the purified enzyme with phenilmethylsulphonyl fluoride. Biochem 1971; 10: 2501-2506
    CrossrefPubMedSuche in Google Scholar
  • 10 Rasmussen PS. Purification of thrombin by chromatography. Biochim Biophys Acta 1955; 16: 156-158
    PubMedSuche in Google Scholar
  • 11 Hirs CH V. Chromatography of enzyme on ion-exchange resins in “Methods in Enzymology”. Lowenstein JM. ed Academic Press; New York: 1955. Vol. 1 pp 113-119
    Suche in Google Scholar
  • 12 Gralnick HR, Givelber HM, Shainoff JR, Finlayson JS. Fibrinogen Bethesda: a congenital dysfibrinogenemia with delayed fibrinopeptide release. J Clin Invest 1971; 50: 1819-1830
    CrossrefPubMedSuche in Google Scholar
  • 13 Ménaché D. Abnormal fibrinogens. Thromb Diath Haemorr 1973; 29: 525-533
    PubMedSuche in Google Scholar
  • 14 Lundblad RL, Utheg LC, Vogel CN, Kingdon HS, Mann KG. Preparation and partial characterization of two forms of bovine thrombin. Biochem Biophys Res Commun 1975; 66: 482-489
    CrossrefPubMedSuche in Google Scholar
  • 15 Fenton JW, Landis BM, Walz DA, Finlayson JS. Human thrombins; in “Chemistry and Biology of Thrombin”. Lundblad RL, Fenton II JW, Mann KG. eds Ann Arbor Science; Ann Arbor: 1977. pp 43-70
    Suche in Google Scholar
  • 16 Fenton JW, Fasco MJ, Stackrow AB, Aronson DL, Young AM, Finlayson JS. Human thrombins. Production, evaluation and proper-ties of α-thrombin J Biol Chem 1977; 252: 3587-3589
    PubMedSuche in Google Scholar
  • 17 Berg W, Hillvam B, Arwin H, Sternberg M, Lundstrom I. The isoelectric point of thrombin and its behaviour compared to pro-thrombin at some solid surfaces. Thromb Haemostas 1979; 42: 972-982
    PubMedSuche in Google Scholar
  • 18 Charo IF, Feinman RD, Detwiler TC. Inhibition of platelet secretion by antagonist of intracellular calcium. J Clin Invest 1977; 60: 866-873
    CrossrefPubMedSuche in Google Scholar