Summary
The mode of action of the antiplatelet agent ticlopidine is not yet fully understood.
Its multiple effects on platelet function include prolongation of the bleeding time,
reduction in primary and secondary Waves of ADP-induced aggregation and inhibition
of collagen and thrombin-induced aggregation. We have studied the in vitro effects of ticlopidine on fibrinogen binding induced by ADP and adrenaline as well
as factor VIII/vWF binding induced by ristocetin.
125I-fibrinogen binding was measured in suspensions of freshly washed normal platelets
stimulated by 10 μM ADP or 10 μM adrenaline. The binding of 125I-factor VIII/vWF in the presence of 1 mg/ml ristocetin was measured in both washed
and paraformaldehyde-fixed platelets. Ticlopidine at final concentrations of 200,
100, 50 and 25 μM inhibited both ADP and adrenaline-induced fibrinogen binding in
a dose-dependent manner. The mean % inhibition of ADP-induced fibrinogen binding was
82, 73, 42 and 32 respectively. The mean % inhibition of adrenaline induced fibrinogen
binding was 86, 82, 60 and 35 respectively. In contrast, the factor VIII/vWF binding
was unaffected by ticlopidine at all concentrations except at 200 μM using fresh platelets
where a slight inhibition (19%) was observed.
These results suggest that ticlopidine either inhibits platelet activation and consequently
fibrinogen binding, or inhibits the binding directly, presumably by having an effect
on the specific configuration of the platelet membrane required for normal fibrinogen
binding.
Keywords
Ticlopidine - Fibrinogen binding - Factor VIII/vWF binding - Platelets
