The Effect of Cod Liver Oil and Corn Oil on Platelets and Vessel Wall in Man

Jan H Brox; Jan-Eirik Killie; Sigurd Gunnes; Arne Nordøy

doi:10.1055/s-0038-1653428

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1981; 46(03): 604-611
DOI: 10.1055/s-0038-1653428

Original Article

Schattauer GmbH Stuttgart

The Effect of Cod Liver Oil and Corn Oil on Platelets and Vessel Wall in Man

Jan H Brox

The Department of Medicine, University Hospital of Tromsø, Tromsø, Norway

,

Jan-Eirik Killie

The Department of Medicine, University Hospital of Tromsø, Tromsø, Norway

,

Sigurd Gunnes

The Department of Medicine, University Hospital of Tromsø, Tromsø, Norway

,

Arne Nordøy

The Department of Medicine, University Hospital of Tromsø, Tromsø, Norway

› Author Affiliations

Abstract

PDF Download

Summary

Ten healthy male subjects on an ordinary diet were given daily dietary supplement of 25 ml cod liver oil (CLO) or corn oil (CO) for periods of 6 weeks in a crossover study. Significant changes were observed in the plasma total fatty acid composition. The main platelet phospholipids fractions were also significantly altered, particularly by CLO with an increase of the eicosapentaenoic acid (EPA): arachidonic acid (AA) ratio. Both supplements reduced collagen induced platelet aggregation and TXB₂ production, with CLO as the most potent one. No spontaneous release of an antiaggregatory substance or 6-keto-PcF_1α from vein tissues were found, and the total urinary excretion of prostaglandin metabolites (E and F series) remained unchanged.

Keywords

Cod liver oil - Corn oil - Platelet aggregation - TXB₂ formation - Prostacyclin formation

PDF (706 kb)

References

References
1 Dyerberg J, Bang HO. Haemostatic function and platelet polyunsatu-rated fatty acids in Eskimos. Lancet 1979; 1: 433
2 Dyerberg J, Bang HO. Lipid metabolism, atherogenesis and haemo-stasis in Eskimos: the role of the prostaglandin-3-family. Haemostasis 1979; 8: 227
3 Needleman P, Sprecher H, Whitaker MO, Wyche A. Mechanisms underlying the inhibition of platelet aggregation by eicosapentaenoic acid and its metabolites. Adv Prostaglandin and Thromboxane Res 1980; 6: 61-68
4 Siess W, Scherer B, Böhlig B, Roth P, Kurzmann I, Weber PC. Platelet-membrane fatty acids, platelet aggregation and thromboxane formation during a mackerel diet. Lancet 1980; 1: 441-444
5 Hornstra G, Haddeman E, ten Hoor F. Fish oils, prostaglandins, and arterial thrombosis. Lancet 1979; 2: 1080
6 Salmon JA. A radioimmunoassay for 6-keto-prostaglandin F_1α . Prostaglandins 1978; 15: 383-397
7 McMillian RM, MacIntyre DE, Gordon JL. Simple, sensitive fluorimetrie assay for malondialdehyde production by blood platelets. Thromb Res 1977; 11: 425-428
8 Rastogi BK, Nordøy A. Lipid composition of cultured human endothelial cells. Thromb Res 1980; 18: 629-641
9 Homstra G, Christ-Hazelhof E, Haddeman E, TenHoor F, Nugteren DH. Fish oil feeding lowers thromboxane and prostacyclin production by rat platelets and aorta and does not result in the formation of prostaglandin I₃ . Prostaglandins. 1981 accepted for publication
10 Nugteren DH. The determination of prostaglandin metabolites in human urine. J Biol chem 1975; 250: 2808-2812
11 Dyerberg J, Bang HO, Hjøme H. Fatty add composition of the plasma lipids in Greenland Eskimos. Am J Clin Nutr 1975; 28: 958-966
12 Whitaker MO, Wyche A, Fitzpatrick F, Sprecher H, Needleman P. Triene prostaglandins: Prostaglandin D₃ and eicosapentaenoic add as potential antithrombotic substances. Proc Natl Acad Sd USA 1979; 76: 5919-5923
13 Jakubowski JA, Ardlie NG. Cyclic AMP-independent inhibition of human platelet function by eicosapentaenoic add. Thromb Res 1980; 17: 751-752
14 Needleman P, Whitaker MO, Wyche A, Watters K, Sprecher H, Raz A. Manipulation of platelet aggregation by prostaglandins and their fatty add precursors: pharmacological basis for a therapeutic approach. Prostaglandins 1980; 19: 165-181
15 Bell RL, Majeras PW. Thrombin-induced hydrolysis of phosphatidylinositol in human platelets. J Biol Chem 1980; 255: 1790-1792
16 Bills TK, Smith JB, Silver MJ. Metabolism of ¹⁴C-arachidonic add by human platelet. Biochim Biophys Acta 1976; 424: 303-314
17 Srivastava KC. Metabolism of arachidonic add by platelets: utilization of arachidonic add by human platelets in presence of linoleic and di-homo-γ-linolenic adds. Z Ernähr Wiss 1978; 17: 248-261
18 Pace-Asciak C, Wolfe LS. Inhibition of prostaglandin synthesis by oleic, linoleic and linoleic adds. Biochim Biophys Acta 1968; 152: 784-787
19 Lands WE M, Le Tellier PR, Rome LH, Vanderhoek JY. Inhibition of prostaglandin biosynthesis. Adv Biosd 1973; 9: 15-28
20 Vallee E, Gougat J, Ageron M. Inhibition of platelet phospholipase A₂ as a mechanism for the anti-aggregating effect of linoleic add. Agents Actions 1980; 10: 57-62
21 Ten HoorF, De Deckere EA M, Haddeman E, Homstra G, Quadt JF A. Dietary manipulation of prostaglandin and thromboxane synthesis in heart, aorta and blood platelets of the rat. Adv Prostaglandin Thromboxane Res 1980; 8: 1771-1781
22 Needleman P, Raz A, Minkes MS, Ferrendell JA, Sprecher H. Triene prostaglandins: Prostacyclin and thromboxane biosynthesis and unique biological properties. Proc Natl Acad Sd USA 1979; 76: 944-948
23 Dyerberg J, Bang HO, Stoffersen E. Eicosapentaenoic add and prevention of thrombosis and atherosclerosis. Lancet. 1978; 2: 117-119
24 Nordøy A, Svensson B, Hoak JC. The effects of albumin bound fatty adds on the platelet inhibitory function of human endothelial cells. Eur J Clin Invest 1979; 9: 5-10
25 Spector AA, Hoak JC, Fry GL, Denning GM. Effect of fatty add modification on prostacyclin production by cultured human endothelial cells. J Clin Invest 1980; 65: 1003-1012
26 Brox J. The effect of incubation fluids on PGI₂ formation in cultured human endothelial cells. In preparation. 1981