Thromb Haemost 1981; 46(03): 612-616
DOI: 10.1055/s-0038-1653429
Original Article
Schattauer GmbH Stuttgart

In Vivo Studies on the Inhibition of Coagulation by Fractionated Heparin and by a Heparin Analogue I. Effects of Heparin Fractions

U Schmitz-Huebner
The Department of Medicine and Institute for Arteriosclerosis Research, University of Münster, Münster, West Germany
,
L Balleisen
The Department of Medicine and Institute for Arteriosclerosis Research, University of Münster, Münster, West Germany
,
F Asbeck
The Department of Medicine and Institute for Arteriosclerosis Research, University of Münster, Münster, West Germany
,
J van de Loo
The Department of Medicine and Institute for Arteriosclerosis Research, University of Münster, Münster, West Germany
› Institutsangaben
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Publikationsverlauf

Received 13. Januar 1981

Accepted 28. Juli 1981

Publikationsdatum:
26. Juli 2018 (online)

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Summary

High and low molecular weight heparin fractions obtained by gel filtration chromatography of sodium mucosal heparin were injected subcutaneously into six healthy volunteers and compared with the unfractionated substance in a cross-over trial. Equal doses of 5,000 U were administered twice daily over a period of three days and heparin activity was repeatedly controlled before and 2, 4, 8 hrs after injection by means of the APTT, the anti-Xa clotting test and a chromogenic substrate assay. In addition, the in vivo effect of subcutaneously administered fractionated heparin on platelet function was examined on three of the volunteers. The results show that s.c. injections of the low molecular weight fraction induced markedly higher anti-Xa activity than injections of the other preparations. At the same time, APTT results did not significantly differ. Unfractionated heparin and the high molecular weight fraction enhanced ADP-induced platelet aggregation and collagen-mediated MDA production, while the low molecular weight fraction hardly affected these assays, but potently inhibited thrombin-induced MDA production. All heparin preparations stimulated the release of platelet Factor 4 in plasma. During the three-day treatment periods, no side-effects and no significant changes in the response to heparin injections were detected.