In Vitro Effect of Ethanol on ADP and Collagen-Induced Platelet Aggregation

Summary

Platelet aggregation was measured by Born’s method. Plasma from Sprague-Dawley rats and inbred albino rabbits was used. 20% ethanol in 0.9%(w/v) NaCl was added to plasma at final concentrations of 56.5 mM, 85.9 mM 171.5 mM and 343.1 mM or to whole blood at final concentrations of 8.7 mM, 17.4 mM, 52.5 mM, 171.5 mM and 343.1 mM. Experiments using 0.9% NaCl volumes equivalent to the added ethanol volume were also conducted. Dose-response aggregation tests showed that a decrease in ADP-and collagen-induced aggregation existed when ethanol was mixed with rat or rabbit blood prior to the preparation of platelet-rich plasma. There was no effect on ADP-induced platelet aggregation when ethanol was mixed with rat or rabbit plasma. Collagen-induced aggregation was impaired only in rat plasma when ethanol concentration reached 343.1 mM. These results suggested that ethanol modified platelet function possibly via red cells.

Keywords

Ethanol - Platelet rich plasma - Blood - Platelet aggregation

• References

• 1 Post R, Desforges J. Thrombocytopenia and alcoholism. Ann Intern Med 1968; 68: 1230-1236
• 2 Post R, Desofrges J. Thrombocytopenic effect of ethanol infusion. Blood 1968; 31: 344-347
• 3 Haut M, Cowan DH. The effect of ethanol on hemostatic properties of human blood platelets. Am J Med 1974; 56: 22-33
• 4 Elmér O, Göransson G, Saku M, Bengmark S. Ethanol-induced microaggregate formation in pig and rabbit blood. An in vitro study Thromb Haemostas 1977; 37: 344-350
• 5 Bengmark S, Göransson G, Idvall J, Zoucas E. Increased bleeding during liver resection in ethanol-intoxicated rats. Thromb Haemostas 1980; 43: 203-207
• 6 Nordensson NG. Klinisk Morfologisk Hematologi. Scandinavian University Books; Stockholm: 1961
• 7 Born BV R. 1962 Quantitative investigation into the aggregation of blood platelets. J Physiol 1962; 162: 67-73
• 8 Zucker MB. Platelet agglutination and vasoconstriction as factors in spontaneous hemostasis in normal, thrombocytopenic, heparinized and hypoprothrombinemic rats. Am J Physiol 1947; 148: 275-282
• 9 Spaet TH. The platelet in hemostasis. Ann N Y Acad Sri 1962; 31: 31-42
• 10 White JG. Combined nephelometric and electron microscope study of the platelet release reaction. Thrombos Diathes Haemorrh 1970; 42 (suppl) 73-91
• 11 Hellem AJ. The adhesiveness of human blood platelets in vitro. Scand J Clin Lab Invest 1960; 12 (suppl) (Suppl. 51) 1-117
• 12 Salzman EW. Measurement of platelet adhesiveness. J Lab. Clin. Med 1963; 62: 724-735
• 13 Bowie WE, Owen J, Thompson CA, Didisheim JH. A test of platelet adhesiveness. Mayo Clin Proc 1969; 44: 306-309
• 14 O’Brien JR, Etherington M, Jamieson S, Ainsworth JF. Stressed template bleeding time and other platelet-function tests in myocardial infarction. Lancet 1963; 1: 694-696
• 15 Swank RL. Adhesiveness of platelets and leucocytes during acute exsanguination. Am J Physiol 1962; 202: 261-264
• 16 Dhall DP, Mathesson NA. Platelet aggregate filtration pressure - a method of measuring platelet aggregation on whole blood. Cardiovasc Res 1969; 3: 155-160
• 17 Bom BV R, Cross MJ. The aggregation of blood platelets. J Physiol 1963; 168: 178-195
• 18 Zweiffler AJ, Sandbar SS. Inhibition of platelet adhesiveness and aggregation by benzylalcohol and phenol. Thrombos Diathes Haemorrh 1969; 21: 362-366
• 19 Davis JW, Phillips PE. The effect of ethanol on human platelet aggregation in vitro. Atherosclerosis 1970; 11: 473-477
• 20 Stuart M. Ethanol-inhibited platelet prostaglandin synthesis in vitro. J Stud Alcohol 1979; 40: 1-6
• 21 Sinakos S, Caen JP. Platelet aggregation in mammalians (human, rat, rabbit, guinea-pig, horse, dog). A comparative study Thrombos Diathes Haemorrh 1967; 17: 99-101
• 22 Chung-Hsin Tsáo. Rat platelet aggregation by ATP. Am J Pathol 1976; 85: 581-592
• 23 Michel H, Caen JP, Born GV R, Miller R, d’Auriac AnglesG, Meyer P. Relation between the inhibition of aggregation and the concentration of cAMP in human and rat platelets. Br J Haematol 1976; 33: 27-38
• 24 Born GV R, Bergqvist D, Arfors KE. Evidence for inhibition of platelet activation in blood by drug effect on erythrocytes. Nature 1976; 259: 223-234