Platelet Surface Charge and Aggregation Effects of Polyelectrolytes

K. A Gröttum

doi:10.1055/s-0038-1653559

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1969; 21(03): 450-462
DOI: 10.1055/s-0038-1653559

Originalarbeiten - Original Articles - Travaux Originaux

Schattauer GmbH

Platelet Surface Charge and Aggregation Effects of Polyelectrolytes

K. A Gröttum M. D.

¹Hematology Section of Medical Department A (Head: Prof. P. F. Hjort), Rikshospitalet, Oslo, Norway

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Abstract

Summary

The effect on platelet surface charge by adding the polybases : Dextran-DEAE and Polybrene and the polyacids : Dextran sulphate, heparin and bovine platelet fibrinogen to platelet-rich plasma was studied by platelet electrophoresis. In small concentrations all the reagents decreased the electrophoretic mobility of platelets, but not that of erythrocytes, suggesting that the polymeric agents were specifically adsorbed to the platelets and thereby reducing their negative surface charge. When the reagents reduced the electrophoretic mobility of the platelets to 85% of normal, platelet aggregation was induced. This effect was probably not mediated through release of ADP, as the presence of ADPase did not modify the effect. The influence of polybases was not inhibited by EDTA. The effect of Dextran sulphate was inhibited by EDTA and increased by small amounts of calcium ions. Increasing concentrations of Dextran sulphate and heparin increased the electrophoretic mobility of platelets, but did not aggregate the platelets. The results suggest that a decrease in the platelet electrostatic potential is essential in platelet aggregation.

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References

References
1 Abramson H. A. The electrophoresis of the blood platelets of the horse with reference to their origin and to thrombus formation. J. exp. Med 47: 677 1928;
2 Bangham A. D, Pethica B. A, Seaman G. V. P. The charged groups at the interface of some blood cells. Biochem. J 69: 12 1958;
3 Born G. V. R. Quantitative investigations into aggregation of blood platelets. J. Physiol. (Lond) 162: 67 1962;
4 Brinton Jr. G. G, Lauffer M. A. The electrophoresis of viruses, bacteria, and cells, and the microscope method of electrophoresis. In: Electrophoresis, Theory, Methods and Applications. ed. by Bier M. 427 Academic Press; New York: 1959
5 Fuhrmann G. F, Ruhenstroth-Bauer G. Cell electrophoresis employing a rectangular cuvette. In: Cell electrophoresis. ed. by Ambrose E. J. 22 J. & A. Churchill; London: 1965
6 Gaarder A, Jonsen J, Laland S, Hellem A, Owren P. A. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature (Lond) 192: 531 1961;
7 Gaarder A, Laland S. Hypothesis for the aggregation of platelets by nucleotides. Nature (Lond) 202: 909 1964;
8 Gröttum K. A. Influence of aggregating agents on electrophoretic mobility of blood platelets from normal individuals and from patients with cardiovascular diseases. Lancet I: 1406 1968;
9 Hampton J. R, Mitchell J. R. A. Effects of aggregating agents on the electrophoretic mobility of human blood platelets. Brit. med. J I: 1074 1966;
10 Haslam R. J. Role of adenosine diphosphate in the aggregation of human blood-platelets by thrombin and fatty acids. Nature (Lond) 202: 765 1964;
11 Hjort P. F, Stormorken H. A study of the in vitro and in vivo effects of a synthetic heparinlike anticoagulant: Dextran sulphate. Scand. J. clin. Lab. Invest. Suppl. 29 1957
12 Holmsen I. In preparation.
13 Hovig T. Release of a platelet-aggregating substance (adenosine diphosphate) from rabbit blood platelets induced by saline “extract” of tendons. Thrombos. Diathes. haemorrh. (Stuttg) 09: 264 1963;
14 KatchalsJcy A, Danon D, Nevo A, DeVries A. Interactions of basic polyelectrolytes with the red blood cell. II. Agglutination of red blood cells by polymeric agents. Biochim. biophys. Acta (Amst) 33: 120 1959;
15 Lopaciuk S, Solum N. O. Purification of bovine blood platelet fibrinogen. (In preparation.)
16 Mitchell J. R. A, Sharp A. A. Platelet clumping in vitro. Brit. J. Haemat 10: 78 1964;
17 Nordling S. Adhesiveness, growth behaviour and charge density of cultured cells. Acta path. microbiol. scand. Suppl. 192 1967
18 Overbeek J. T. G, Lijklema J. Electrical potentials in colloid systems. In: Electrophoresis, Theory, Methods, and Applications. ed. by Bier M. 16 Academic Press; New York: 1959
19 Pfleiderer T, Brossmer R. Zum Mechanismus der Thrombozytenaggregation. II. Erhöhung der Klebrigkeit durch geladene Makromoleküle. Thrombos. Diathes. haemorrh. (Stuttg) 18: 673 1967;
20 Pollack W, Hager H. J, Reckel R, Toren D. A, Singher H. O. Study of the forces involved in the second stage of haemagglutination. Transfusion 05: 158 1965;
21 Salzman E. W. Studies on the mechanism of platelet aggregation by ADP. Thrombos. Diathes. haemorrh. (Stuttg) 15: 628 1966;
22 Seaman G. V. F, Vassar P. S. Changes in the electrokinetic properties of platelets during their aggregation. Arch. Biochem 117: 10 1966;
23 von Smoluchowski M. Handbuch der Elektrizität und des Magnetismus. ed. by Graetz II. L. 366 J. A. Barth, Leipzig. 1921
24 Solum N. O. Aggregation of human platelets by preparations of bovine platelet fibrinogen. (In preparation.)
25 Spaet T. H, Cintron J. Induction of adenosine diphosphate release from human platelets by connective tissue fragments. Thrombos. Diathes. heamorrh. (Stuttg.) Suppl 13: 335 1963;
26 Spaet T. H, Lejnicks J. Studies on the mechanism whereby platelets are clumped by adenosine diphosphate. Thrombos. Diathes. haemorrh. (Stuttg) 15: 36 1966;