An Accelerated Red-Cell Hemagglutination Inhibition Immunoassay for Measuring Fibrinogen Degradation Products in Human Serum

R Fischer; M Furlan; E. A Beck

doi:10.1055/s-0038-1653678

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1971; 26(02): 311-314
DOI: 10.1055/s-0038-1653678

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

An Accelerated Red-Cell Hemagglutination Inhibition Immunoassay for Measuring Fibrinogen Degradation Products in Human Serum

R Fischer

¹Central Hematology Laboratory, Inselspital, and University of Berne, School of Medicine, Berne, Switzerland

,

M Furlan

¹Central Hematology Laboratory, Inselspital, and University of Berne, School of Medicine, Berne, Switzerland

,

E. A Beck^*

¹Central Hematology Laboratory, Inselspital, and University of Berne, School of Medicine, Berne, Switzerland

› Institutsangaben

Abstract

Summary

A further modification of Merskey’s hemagglutination inhibition assay for measurement of fibrinogen degradation products (FDP) in human serum was developed. Blood is clotted in the presence of protamine chloride (25 u/ml), in addition to thrombin and s-aminocaproic acid, and at room temperature. Defibrination is complete within 5 min even in blood obtained from patients on heparin treatment. The hemagglutination immunoassay was also considerably accelerated by immediate addition of sensitized red cells to the mixtures of antiserum and antigen dilutions at 37° C. Accurate readings of FDP titers may thus be obtained within 20 min following venipuncture.

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Referenzen

References
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