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Thromb Haemost 1971; 26(03): 512-522
DOI: 10.1055/s-0038-1653704
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Streptokinase and Urokinase Activation of Human, Chimpanzee and Baboon Plasminogen

P. A McKee   *
1   Department of Medicine, Veterans Administration Hospital, Durham, North Carolina 27706
,
W. B Lemmon
3   Department of Psychology, University of Oklahoma, Norman, Oklahoma 73069
,
J. W Hampton   **
2   Department of Medicine, University of Oklahoma Medical Center, Oklahoma City, Oklahoma 73105
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Publikationsdatum:
24. Juli 2018 (online)

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Summary

Human, chimpanzee and baboon plasminogens, in that order, were found to be decreasingly susceptible to streptokinase (SK) activation. The relative activation of the three primate plasminogens remained the same when species specific fibrin or casein was used as substrate and when the following conditions were varied: 1. SK concentration; 2. plasminogen concentration; and 3. length of incubation of the SK-plasminogen-substrate reaction mixture. Under the same conditions, the three primate plasminogens were readily activated by human urokinase (UK) and no consistent order of activation by UK was observed. Our findings suggest that the site on plasminogen which interacts with SK has undergone changes during evolution whereas the site at which UK activates each of the three plasminogens has changed little. It remains obscure whether the susceptibility of primate plasminogen to SK activation is a specifically selected genetic trait or is simply a laboratory indication of structural changes in the plasminogen molecule that have occurred for other reasons.

* Present address : Department of Medicine, Duke University Medical Center, Durham, North Carolina 27706.


** Recipient of a Career Development Award, National H eart Institute, National I nstitutes of Health.


 

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