Mechanisms of Interaction between a Phosphoinositide Anticoagulant and a Phospholipid Activator in the Stypven Type Conversion of Prothrombin to Thrombin

Summary and Conclusion

In the Stypven activation of prothrombin to thrombin, successive steps were investigated in order to locate more precisely the action of a phosphoinositide anticoagulant. The anticoagulant effect has been located precisety at the time of interaction of activated factors X and V, calcium ions and phospholipid material. When this interaction is completed, the phosphoinositide is no longer capable of demonstrating anticoagulant activity.

• References

• 1 Bourgain R. P.L, Kahn M. J. P. In press.
• 2 Kahn M. J. P, Bourgain R. H. A simplified preparation procedure of factor X by paper electrophoresis. Hemostase V: 413 1965;
• 3 Shinowara G. Enzyme studies on Human Blood. 5°: Estimation of prothrombin by an homologous isolation method. Amer. J. med. technol 159: 306 1949;
• 4 Biggs R, Macfarlane R. G. Human Blood Coagulation and its disorders. 4. ed.. Blackwell; Oxford: 1965
• 5 Cole E. R, Koppel J. L, Olwin J. H. Phospholipid-Protein Interactions in the Formation of Prothrombin Activator. Thrombos. Diathes. haemorrh. (Stuttg.) XIV: 431 1965;
• 6 Williams W. J. The activity of lung microsomes in blood coagulation. J. biol. Chem 239: 933 1964;
• 7 Cole E. R, Koppel J. L, Olwin J. H. Interaction of bovine autoprothrombin C with phospholipid and divalent cation. Canad. J. Biochem 42: 1595 1964;
• 8 BrecJcenridge R. T, Ratnoff O. D. The activation of human proaccelerine by Stuart factor. Proc. Cent. Soc. clin. Res 37: 14 1964;