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Thromb Haemost 1970; 17(03): 593-600
DOI: 10.1055/s-0038-1654190
Originalarbeiten – Original Articles – Travaux Originaux
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Coagulation Findings in Rats with Experimental Hypersplenism and Their Changes after Splenectomy and after Administration of Adrenaline

P Pudlák
1   Institute of Hematology and Blood Transfusion (Director: Prof .Dr. Jaroslav Hořejši, Dr. Sc.), Prague
,
I Farská
1   Institute of Hematology and Blood Transfusion (Director: Prof .Dr. Jaroslav Hořejši, Dr. Sc.), Prague
,
V Brabec
1   Institute of Hematology and Blood Transfusion (Director: Prof .Dr. Jaroslav Hořejši, Dr. Sc.), Prague
,
V Pospíšilová
1   Institute of Hematology and Blood Transfusion (Director: Prof .Dr. Jaroslav Hořejši, Dr. Sc.), Prague
› Author Affiliations
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Publication Date:
27 June 2018 (online)

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Summary

1. The following coagulation changes were found in rats with experimental hypersplenism: a mild prolongation of the recalcification time, shortened times in Quick’s test, a lowered activity in plasma thrombin time and shortened times in the partial thromboplastin test. Concentrations of factor II, V, VII (+X), VIII and X did not differ from those of normal control rats.

2. The administration of adrenaline to hypersplenic rats induced the correction of the partial thromboplastin test, Quick’s test and plasma thrombin time to normal values. Concentrations of coagulation factors were not significantly changed. An increase was found in factor V.

3. Splenectomy performed in hypersplenic rats was followed by a shortened recalcification time, a prolongation of the partial thromboplastin test and of the test with partial thromboplastin and kaolin. A prolongation was also observed in Quick’s test. Complete correction of plasma thrombin time was not observed. The concentration of factor VII increased.

4. The administration of adrenaline to splenectomized rats with experimental hypersplenism did not induce any significant changes with the exception of a corrected plasma thrombin time and a decreased concentration of factor VIII.

5. A different reaction of factor VIII to adrenaline in normal and hypersplenic rats is pointed out.

 

  • References

  • 1 Bachmann F, Duckert F, Koller F. The Stuart — Prower factor assay and its clinical significance. Thrombos. Diathes. haemorrh. (Stuttg.) 2: 24 1958;
    PubMedSearch in Google Scholar
  • 2 Bell W. N, Alton H. G. A brain extract as a substitute for platelet suspensions in the thromboplastin generation test. Nature (Lond.) 174: 880 1954;
    CrossrefPubMedSearch in Google Scholar
  • 3 Benkö S. Reticuloses and experimentally induced reticuloses due to storage of methyl-cellulose. Acta med. acad. Sci. hung 12: 115 1958;
    PubMedSearch in Google Scholar
  • 4 Brabec V, Pospíšilová V, Šebestík V, Jircisek A. Der experimentelle Hypersplenismus. Hämatologische Veränderungen bei dem »makromolekularen Syndrom« der Vistar-Ratten. Haematologia (Budapest) 3: 345 1969;
    PubMedSearch in Google Scholar
  • 5 Gaynor E, Spaet T. H. Failure of methyl palmitate induced reticuloendothelial blockade to affect plasma levels of factor VIII. Blood 28: 595 1966;
    PubMedSearch in Google Scholar
  • 6 Geller L. I. Fiziologia i patologia selezenki. Medicina; Moskva: 1964
    Search in Google Scholar
  • 7 Giblett E. R, Motulski A. G, Casserd F, Houghton B, Finch G. A. Studies on the pathogenesis of splenic anemia. Blood 11: 118 1956;
    PubMedSearch in Google Scholar
  • 8 Goudemand M, Foucaut M, Habay D, Parquet-Gernez A. Les variations du taux de facteur VIII au cours de l’exercise musculaire. Essai d’interprétation. Nouv. Rev. franç. Hémat 4: 315 1964;
    PubMedSearch in Google Scholar
  • 9 Ingram G. I. G. Increase in antihaemophilic globulin activity following infusion of adrenaline. J. Physiol 156: 217 1961;
    CrossrefPubMedSearch in Google Scholar
  • 10 Lee L, Prose P. H, Cohen M. H. The role of the reticuloendothelial system in diffuse, low grade intravascular coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 20: 87 1966;
    PubMedSearch in Google Scholar
  • 11 Libre E. P, Cowan D. H, Watkins S. P. J. Relationships between spleen, platelets and factor VIIIlevels. Blood 31: 358 1968;
    PubMedSearch in Google Scholar
  • 12 Naleczyňska A, Lopaciuk St, Pawelski St. Wplyw adrenaliny na poziom czynnika VIII (AHG-) w osoczu osób zdrowych. Pol. Arch. Med. Wewn 35: 807 1965;
    PubMedSearch in Google Scholar
  • 13 Palmer J. G, Eichwald E. J, Cartwright G. E, Wintrobe M. M. The experimental production of splenomegaly, anemia and leukopenia in albino rats. Blood 8: 72 1953;
    PubMedSearch in Google Scholar
  • 14 Pool J. G, Spaet T. H. Ethionine induced depression of plasma antihemophilic globulin. Proc. Soc. exp. Biol. (N. Y.) 87: 54 1954;
    CrossrefPubMedSearch in Google Scholar
  • 15 Pudlák P. O vyznamu koncentrace a aktivity tkáňového tromboplastinu. Čas. lék. čes 100: 1409 1961;
    PubMedSearch in Google Scholar
  • 16 Pudlák P, Deimlová E. Quantitative assay of factor VIII (antihaemophilic globulin) using the thrombin generation test. Brit. J. Haemat 12: 409 1966;
    CrossrefPubMedSearch in Google Scholar
  • 17 Pudlák P, Farská I, Deimlová E. Changes in factor V and VIII after adrenaline in normal and splenectomized rats. Brit. J. Haemat 16: 373 1969;
    CrossrefPubMedSearch in Google Scholar
  • 18 Pudlák P, Farská I, Horák J, Deimlovci E. Changements de l’activité de la globuline anti-hémophilique (facteur VIII) après administration d’adrénaline aux malades avec affections du système retículo-endothelial. Nouv. Rév. franç. Hémat 8: 247 1968;
    PubMedSearch in Google Scholar
  • 19 Quick A. J. The prothrombin in hemophilia and obstruktive jaundice. J. biol. Chem 109: LXXIII 1935;
    PubMedSearch in Google Scholar
  • 20 Roth Z, Josífko M, Malí V, Trcka V. Statistické metody v experimentam! medicine. Státní zdravotnické nakladatelství, Praha 1962.
    PubMed
  • 21 Soulier J. P. Étude analytique du temps de Quick allongé. Dosage de prothrombine, de proconvertine et de proaccelérine. Sang 23: 549 1952;
    PubMedSearch in Google Scholar
  • 22 Spaet T. H. Studies on the in vivo behavior of blood coagulation product I in rats. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 276 1962;
    PubMedSearch in Google Scholar
  • 23 Spaet T. H, Horowitz H. I, Zucker-Franklin D. Reticuloendothelial clearance of blood thromboplastin. J. clin. Invest 39: 1029 1960;
    PubMedSearch in Google Scholar
  • 24 Spaet T. H, Horowitz H. I, Zucker-Franklin D, Cintron J, Biezenski J. J. Reticuloendothelial clearance of blood thromboplastin by rats. Blood 17: 196 1961;
    PubMedSearch in Google Scholar
  • 25 Spala M. Odběr krve u krys punkcí brišní aorty. Čs. fysiol. (In Press.)
    PubMed