Effects of Chemical Compounds upon Current Induced Thrombosis in Rat Mesenteric Vessels

Summary

From previous work in our laboratories, there is a large amount of evidence for an electrochemical mechanism of thrombosis. The blood vessel wall and blood cells are negatively charged under normal conditions. Antithrombogenic drugs increase their negative charge densities while thrombogenic drugs have the opposite effect. Injury makes the blood vessel wall less negatively charged or even positively charged. There is a relation between vascular injury currents and thrombosis. In the present study, the effect of certain chemical agents upon in situ thrombosis in rat mesoappendix vessels, produced by an applied electric current of the sign and magnitude of physiological injury currents, is examined. Compounds such as polyquarternary, with basic groups enhance thrombosis while compounds such as heparin with acidic groups inhibit thrombosis.

^* Recipient of Career Scientist Award, Health Research Council of the City of New York (Contract No. I 542).

• References

• 1 Sawyer P. N, Paie J. W. Electrical potentials differences across normal aorta and aortic grafts of dogs. Amer. J. Physiol 175: 113 1953;
• 2 Abramson H. A. The cataphoretic velocity of mammaliam red blood cells. J. gen. Physiol 12: 711 1929;
• 3 Sawyer P. N, Pate J. W. Bioelectric phenomena as an etiologic factor in intravascular thrombosis. Amer. J. Physiol 175: 103 1953;
• 4 Sawyer P. N, Pate J. W, Weldon C. S. Relations of abnormal and injury electric potential differences to intravascular thrombosis. Amer. J. Physiol 175: 108 1953;
• 5 Sawyer P. N, Pate J. W. Bioelectric phenomena as etiological agents in intravascular thrombosis. Surgery 34: 491 1953;
• 6 Sawyer P. N, Deutch B, Pate J. W. The relationship of bioelectric phenomena and small electric currents to intravascular thrombosis. Thrombosis and Embolism, Proc. Int’l. Conf. on Thromb. and Emb. Basel, Switz., 1955.
• 7 Sawyer P. N, Suckling E. E, Wesolowski S. Effects of small electric currents on intravascular thrombosis in the visualized rat mesentery. Amer. J. Physiol 198: 1006 1960;
• 8 Sawyer P. N, Wesolowski S. The electric current of injured tissue and vascular occlusion. Ann. Surg 155: 34 1961;
• 9 Abramson H. A. The possible relationship between the current of injury and white blood cells in inflammation. Amer. J. Med. Sci 167: 702 1924;
• 10 Abramson H. A. The influence of low electromotive force on the electrophoresis of lymphocytes of different ages. J. exp. Med 41: 445 1925;
• 11 Abramson H. A. The electrophoresis of the blood platelets of the horse with reference to their origin and to thrombus formation,. J. exp. Med 47: 677 1928;
• 12 Lustrin I, Breen H, Readon J, Wesolowski S. A, Sawyer P. N. Comparative anticoagulant effects of Coumadin, heparin, and fibrinolysin on direct current thrombosis of rat mesoappendix vessels. Surgery 58: 857 1965;
• 13 Srinivasan S, Aaron R. K, Chopra P. S, Lucas T, Sawyer P. N. Effect of thrombotic and antithrombotic drugs on the surface charge characteristics of canine blood vessels: in vivo and m vitro studies. Surgery 64: 827 1968;
• 14 Srinivasan S, Sawyer P. N. Electrochemical Techniques for studies on intravascular thrombosis. JAAMI 3: 116 1969;