Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1970; 24(01/02): 206-213DOI: 10.1055/s-0038-1654226 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Characteristics of Platelet Factor 3 Release during ADP-Induced AggregationComparison with 5 Hydroxytryptamine ReleaseAuthors Author Affiliations J. J Sixma 1 Department of Internal Medicine (Heads: Prof. Dr. F. L. J. Jordan and Prof. Dr. L. A. Hulst), State University Hospital, Utrecht, The Netherlands J. G Nijessen 1 Department of Internal Medicine (Heads: Prof. Dr. F. L. J. Jordan and Prof. Dr. L. A. Hulst), State University Hospital, Utrecht, The Netherlands Recommend Article Abstract Buy Article(opens in new window) Summary Comparison of serotonin release with PF 3 release during ADP-induced aggregation revealed that the two release reactions are entirely different processes. Serotonin release was found to occur at temperature above 33° C; the optimal temperature was 43° C and maximum release occurred 3 min after addition of ADP. PF 3 release was slow and linear with time occurring both at 37° C and 20° C but it was more prolonged at 20° C. Serotonin release as well as PF 3 release were neglibible in reversible aggregation. The influence of surface contact and stirring speed indicated the significance of mechanical trauma in PF 3 release. In the discussion, mention is made of the possibility that the peripheral balloon-shaped platelet protrusions are subject to membrane changes and thus lead to PF 3 release. Full Text References References Cohen Ph. Relationship between membrane function and permeability. Brit. J. Haemat 13: 739 1967; Hardisty R. M, Hutton R. A. Platelet Aggregation and the Availibility of Platelet factor 3. Brit. J. Haemat 12: 764 1966; Horowitz H. I, Papayoanou M. F. Activation of Platelet factor 3 by adenosine 5’ diphosphate. Thrombos. Diathes. haemorrh. (Stuttg) 19: 18 1968; Marcus A. J, Zucker-Franklin D, Safier L. B, Ullman H. Studies on human platelet granules and membranes. J. clin. Invest 45: 14 1966; Mills D. C. D, Robb I. A, Roberts G. C. K. Release of nucleotides, 5 hydroxytryptamine and enzymes from human blood platelets during aggregation. J. Physiol 195: 715 1968; Sixma J. J, Geuze J. J. Degranulation of Human Platelets during ADP induced Aggregation. Vox Sang 75: 309 1968; Spaet T. H, Cintron J. Studies on platelet factor-3 availibility. Brit. J. Haemat 11: 269 1965; Valdorf-Hansen J. F, Zucker M. B. Abstr. of Communication presented at XIth Congress of Int. Soc. Haemat. New York: 1968 Valdorf-Hansen J. F, Zucker M. B. Personal communication. 1969 Zucker M. B, Peterson J. Inhibition of Adenosine diphosphate, induced secondary aggregation and other platelet functions by acetyl salicylic acid ingestion. Proc. Soc. Exp. Biol. (N. Y) 127: 547 1968;
