Junction Thrombi Deposited on Conducting Materials at Different Potentials

M Ranganathan; J. G Stempak; S Srinivasan; P. N Sawyer

doi:10.1055/s-0038-1654234

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1970; 24(01/02): 273-285
DOI: 10.1055/s-0038-1654234

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Junction Thrombi Deposited on Conducting Materials at Different Potentials

An Electron Microscopic Study

M Ranganathan^*)

¹Electrochemistry and Biophysical Laboratories of the Department of Surgery and Surgical Research, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

,

J. G Stempak^**)

¹Electrochemistry and Biophysical Laboratories of the Department of Surgery and Surgical Research, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

,

S Srinivasan^***)

¹Electrochemistry and Biophysical Laboratories of the Department of Surgery and Surgical Research, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

,

P. N Sawyer

¹Electrochemistry and Biophysical Laboratories of the Department of Surgery and Surgical Research, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

› Author Affiliations

Abstract

Summary

The present study reveals that the deposits on metal prostheses maintained at positive (spontaneous) or negative electric potentials are in histologic composition and characteristics of the nature of a thrombus and not of a clot in vitro. The study also shows that thrombic deposits on copper prostheses maintained at negativ potentials are broadly similar in nature and composition though there are some significant distinctions among them. In the study on the sample from a copper prosthesis maintained at a potential of —160 mv where only junctional thrombi were formed, it is observed that within about 200 μl from the prosthetic surface the pattern of composition of thrombus changes. The predominant platelet population with very few erythrocytes and fibrin near the prosthetic surface changes with gradual increase in number of red blood cells into a region predominantly composed of red blood cells. Several distorted blood elements with electron dense inclusions in them, observed close to the prosthetic surface, were more frequently observed in the sample from the copper prosthesis at a potential of —160 mv than in the sample at —110 mv.

Full Text

References

References
1 Wiener J, Spiro D. Electron Microscopic Studies in Experimental Thrombosis. Exp. molec. Path 02: 558 1962;
2 Jorgensen L, Rowsell H. G, Hovig T, Mustard J. F. Fate of platelet mural thrombi in some arteries. Circulation 32 Suppl.II: 19 1965;
3 Still W. J. S, Ghani A. R, Dennison S. M. The Organization of isolated mural thrombi in aortic grafts an electron microscopic study. Amer. J. Path 51: 1013 1967;
4 O’Neal R. M, Jordan Jr G. L, DeBakey M. E, Halpert B. Cells grown on isolated intravascular dacron hubs; an electron microscopic study. Exp. molec. Path 03: 403 1964;
5 Sawyer P. N. Biophysical Mechanisms in Vascular Homeostasis and Intravascular Thrombosis. Appleton-Century Crofts; N.Y: 1965
6 Sawyer P. N, Srinivasan S. Metallic and plastic prosthetic devices as vascular wall substitutes. J. Biomed. Mater. Res 01: 83 1967;
7 Sawyer P. N, Srinivasan S. Studies on the Biophysics of Intravascular Thrombosis. Amer. J. Surg 113: 42 1967;
8 Sabatini D. D, Bensch K, Barrnett R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzyme activity by aldehyde fixation. J. Ce.. Biol 17: 19 1963;
9 Millonig G. In: Electron Microscopy. Proc. 5th International Congress of Electron Microscopy. Vol. 2 8 Academic Press; N.Y: 1962
10 Luft L. H. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 09: 409 1961;
11 Trump B. F, Smuckler E. A, Benditt E. P. A Method for Staining Epoxy Sections for Light Microscopy. J. Ultrastr. Res 05: 343 1961;
12 Stempak J. G, Ward R. T. An Improved Staining Method for Electron Microscopy. J. Cell Biol 22: 679 1964;
13 Reynolds E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol 17: 208 1963;
14 McKay D. G. Blood Coagulation and the Generalized Shwartzman Reaction. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 20: 107 1966;
15 McKay D. G, Muller-Berghaus G, Cruse V. Activation of Hageman Factor by Ellagic Acid and the Generalized Shwartzman Reaction. Amer. J. Path 54 (03) 393 1969;
16 Shirley Johnson A, Balboa R. S, Pederson H. J, Buckley M. The Ultrastructure of platelet participation in Hemostasis. Thrombos. Diathes. haemorrh. (Stuttg) 13: 65 1965;
17 Jorgensen L, Rowsell H. G, Hovig T, Mustard J. F. Resolution and Organization of Platelet-rich mural thrombi in carotid arteries of swine. Amer. J. Path 51 (05) 681 1967;
18 Still W. J. S, Blout E. H. Electronmicroscopic Appearance of Fibrin in Thin Sections. Nature (Lond) 179: 868 1957;
19 Macher E. Licht- u. elektronenmikroskopische Befunde in Beziehung zur Atiopathogenese. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 22: 87 1967;
20 Messen H, Schulz H. Contribution to Morphology of Thrombosis. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 21: 19 1966;
21 Mustard J. F, Jorgensen L, Hovig T, Glynn M. F, Rowsell H. C. Role of platelets in thrombosis. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 21: 131 1966;
22 Hovig T, Dodds W. J, Rowsell H. G, Mustard J. F. The Transformation of Hemostatic Plugs in Normal and Factor IX deficient Dogs. Amer. J. Path 53 (03) 355 1968;
23 Behnke O. Further studies on microtubules in thrombocytes. J. Ultrast. Res 13: 469 1965;
24 Hayden G. B, Tayler D. A. Microtubules in hamster platelets. J. Cell Biol 26: 673 1965;
25 Hayem G. Recherches sur L’Evolution Des Hematies Etc. Arch. Physiol. Norm. Et. Pathol. Series 02: 692 1878;
26 Wright J. H, Minot G. R. The viscous metamorphosis of the blood platelets. J. exp. Med 26: 395 1917;
27 Zucker M. B. Platelet agglutination and vasoconstriction as factors in spontaneous hemostasis in normal, thrombocytopenic, heparinized and hypoprothrombinémie rats. Amer. J. Physiol 148: 215 1947;
28 Bergsagel D. E. Viscous metamorphosis of platelets : Morphological platelet changes induced by an intermediate product of blood thromboplastin formation. Brit. J. Haemat 02: 130 1956;
29 Poole J. C. F, French J. E. Thrombosis-A review. J. atheroscler. Res 01: 251 1961;
30 Poole J. C. F, French J. E, Cliff W. J. The early stages of thrombosis. J. clin. Path 16: 523 1963;