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Thromb Haemost 1970; 24(03/04): 463-474
DOI: 10.1055/s-0038-1654256
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Urokinase-Induced Fibrinolysis of 125I-Fibrin

Conditions for First-Order Activation Kinetics, Density-Gradient Separation of Split Products, and a Microassay[*]
Anne P. Ball
1   Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA)
,
Eugene D. Day
1   Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA)
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Publication Date:
28 June 2018 (online)

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Summary

By means of the density-gradient separation of soluble fibrin split products from 125I-labeled fibrin in 0.8-ml gradients, a micro-technique for the measurement of urokinase activation of plasminogen was developed. The conditions necessary to maintain first-order activation kinetics were obtained and incorporated into the micro-technique, thus providing a linear micro-assay of 0.1-ml volumes of urokinase in the range of 2.5-50.0 CTA units.

** Supported by Contract AT-(40-l)-3195 with the U.S. Atomic Energy Commission and by Grants HE-08929 and NB-06233 with the National Institutes of Health, USPHS.


** This work is taken from a dissertation submitted in partial fulfillment of the Ph.D. degree, Duke University. Present address, Department of Surgery, Duke University Medical Center.


 

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