Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1970; 24(03/04): 463-474DOI: 10.1055/s-0038-1654256 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Urokinase-Induced Fibrinolysis of 125I-Fibrin Conditions for First-Order Activation Kinetics, Density-Gradient Separation of Split Products, and a Microassay[*] Anne P. Ball 1 Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA) , Eugene D. Day 1 Departments of Microbiology and Immunology and of Surgery, Duke University Medical Center Durham, N.C. 27706 (USA) › Institutsangaben Artikel empfehlen Abstract Artikel einzeln kaufen Summary By means of the density-gradient separation of soluble fibrin split products from 125I-labeled fibrin in 0.8-ml gradients, a micro-technique for the measurement of urokinase activation of plasminogen was developed. The conditions necessary to maintain first-order activation kinetics were obtained and incorporated into the micro-technique, thus providing a linear micro-assay of 0.1-ml volumes of urokinase in the range of 2.5-50.0 CTA units. Volltext Referenzen References 1 Alkjaersig N, Fletcher A. P, Sherry S. The mechanism of clot dissolution by plasmin. J. clin. Invest 38: 1086 1959; 2 Berg W. Plasminogen and urokinase determination based on the lysis time method. Acta med. scand Suppl. 478 1967; 3 Clement W. E, McNicol G. P. The preparation and use of I131-tagged fibrinogen to demonstrate fibrinolysis. J. clin. Pathol 12: 544 1959; 4 de Wit Dudok G. D, Krijnen H. W, den Ottolander G. J. H. The measurement of fibrinolytic activity with 131I-labeled clots. I. The Methods. Thrombos. Diathes. haemorrh. (Stuttg) 08: 315 1962; 5 Eisen H. N, Keston A. S. The immunologic reactivity of bovine serum albumin labeled with trace amounts of radioactive iodine (I131). J. Immunol 63: 71 1949; 6 Fritz R. B, Lassiter S, Day E. D. A quantitative precipitin study of porcine fibrinogen employing paired labels. J. Immunol 98: 1213 1967; 7 Kline D. L. Purification and crystallization of plasminogen (profibrinolysin). J. biol. Chem 204: 949 1953; 8 Laki K. The polymerization of proteins: The action of thrombin on fibrinogen. Arch. Biochem 32: 317 1951; 9 McNicol G. P, Douglas A. S, Bayley C. Experience with streptokinase infusions. Lancet II: 1297 1962; 10 Merrills R. J, Shaw J. T. B. Kinetics of fibrin clot lysis. Biochem. J 106: 101 1968; 11 Remmert L. F, Cohen P. Partial purification and properties of a proteolytic enzyme of human serum. J. biol. Chem 181: 431 1949; 12 Robbins K. G, Summaria L. Purification of human plasminogen and plasmin by gel filtration on Sephadex and Chromatography on Diethylaminoethyl-Sephadex. J. biol. Chem 238: 952 1963; 13 Sawyer W. D, Fletcher A. P, Alkjaersig N, Sherry S. Studies on the thrombolytic activity of human plasma. J. clin. Invest 38: 426 1959; 14 Shulman N. R, Tagnon H. J. Proteolytic activity determined with a substrate tagged with radioactive iodine. J. biol. Chem 186: 69 1950;
