Download PDF
Thromb Haemost 1970; 24(03/04): 543-547
DOI: 10.1055/s-0038-1654264
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Isolation and Purification of Antiplasmins from Human Granulocytes[*]

N Wołosowicz
1   Department of Physiological Chemistry, Medical School, Bialystoic, Poland
,
J Prokopowicz
1   Department of Physiological Chemistry, Medical School, Bialystoic, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
28 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

Two antiplasmins were found in human granulocytes. Chromatography on Sephadex G-200 showed 3 peaks with antiplasmin activity. One immediately acting antiplasmin and 2 progressively acting antiplasmin (I and II) have been identified. The immediate antiplasmin was purified 92-fold, the progressive antiplasmin 1129-fold and progressive antiplasmin II 185-fold. Disc electrophoresis showed homogeneity of the fractions. They were found to migrate with the alpha-2 globulin fraction. The physiological function of the antiplasmins is briefly discussed.

* In part supported by the Polish Academy of Scien ces, Department II.


 

  • References

  • 1 Ambrus C. M, Markus F. Fibrinolytic effect of plasmin antiplasmin complex as reservoir of fibrinolytic enzyme. Amer. J. Physiol 199: 491 1960;
    PubMedSearch in Google Scholar
  • 2 Clarke J. T. L. In: Gel electrophoresis. Whiple H. E. (ed.). Ann. N.Y. Acad. Sci. 121: art. 2, 428 1964
    Search in Google Scholar
  • 3 Guest M. M, Daly B. M, Ware A. G, Seegers W. H. A study of the antifibrinolysin activity in the plasma of various animal species. J. clin. Invest 27: 785 1948;
    CrossrefPubMedSearch in Google Scholar
  • 4 Jacobsson K. Studies on the trypsin and plasmin inhibitors in human blood serum. Scand. J. clin. Lab. Invest 07 Suppl. 14, 55 1955;
    CrossrefPubMedSearch in Google Scholar
  • 5 Kekwick R. A, Mackay N. E, Nance M. H. The purification of human fibrinogen. Biochem. J 60: 11 1955;
    PubMedSearch in Google Scholar
  • 6 Loomis E. G, Ryder A, George Jr G. Fibrinolysin and antifibrinolysin: Biochemical concentration of antifibrinolysin. Arch. Biochem 20: 444 1949;
    PubMedSearch in Google Scholar
  • 7 Nanninga L. B, Guest M. M. On the interaction of fibrinolysin (plasmin) with the inhibitors antifibrinolysin and soybean trypsin inhibitor. Arch. Biochem 106: 542 1964;
    PubMedSearch in Google Scholar
  • 8 Norman P. S. Studies of the plasmin system. II. Inhibition of plasmin by serum or plasma. J. exp. Med 108: 53 1958;
    PubMedSearch in Google Scholar
  • 9 Norman P. S, Hill B. M. Studies of the plasmin system. III. Physical properties of the two plasmin inhibitors in plasma. J. exp. Med 108: 639 1958;
    PubMedSearch in Google Scholar
  • 10 Prokopowicz J. Distribution of fibrinolytic and proteolytic enzymes in subcellular fractions of human granulocytes. Thrombos. Diathes. haemorrh. (Stuttg) 19: 84 1968;
    PubMedSearch in Google Scholar
  • 11 Schwick H. G. Biochemie der Fibrinolyse. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 22: 27 1967;
    PubMedSearch in Google Scholar