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Thromb Haemost 1971; 25(02): 268-278
DOI: 10.1055/s-0038-1654301
Original Articles
Schattauer GmbH

Gel Filtration

A New Technique for Separation of Blood Platelets from Plasma
O Tangen   *)
1   Department of Biology, Boston University, Boston, Massachusetts, U.S.A
,
H. J Berman
1   Department of Biology, Boston University, Boston, Massachusetts, U.S.A
,
P Marfey   **)
1   Department of Biology, Boston University, Boston, Massachusetts, U.S.A
› Author AffiliationsThis study was supported in part by Grant HE 902 from the National Heart Institute, NIH, and Contract DA-49-193-MD-2696 from the Department of the Army, Office of the Surgeon General.
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

Hamster platelets have been separated from their plasmatic enviroment by means of gel filtration. This method of separation gave an excellent yield of platelets free of non-adsorbed plasma proteins.

Gel filtered platelets (GFP), and platelets washed by repeated centrifugations and resuspensions (CP) were compared to each other and to platelets left undisturbed in their native plasma (PRP). Only slight morphological differences between the three platelet preparations could be detected by phase microscopy. In the concentration range of .28–.71 μM ADP, GFP, resuspended in PPP, showed the same degree of aggregation as did PRP. At lower concentrations of ADP, GFP aggregated to a lesser degree than did PRP. CP exhibited aggregation comparable to PRP only at higher concentrations of ADP (.71–1.42 (μM ADP). GFP also supported clot retraction to the same extent as did PRP.

It would appear that gel filtration provides a new, gentle and rapid method for separation, from plasma, of platelets with activity very similar to that of platelets in PRP.

*) Present address:Department of Experimental Medicine, Pharmacia AB, Uppsala, Sweden


**) Department of Biological Sciences, State University of New York at Albany, Albany, New York


 

  • References

  • 1 Arfors K-E, Dhall D. P, Engeset J, Hint H, Matheson N. A, Tangen O. Biolaser endothelial trauma as a means of quantifying platelet activity in vivo. Nature (Lond.) 218: 887-888 1968;
    CrossrefPubMedSearch in Google Scholar
  • 2 Berman H. J. Anticoagulant-induced alterations in hemostasis, platelet thrombosis, and vascular fragility in the peripheral vessels of the hamster cheek pouch. In: Anticoagulants and Fibrinolysins. Ed. MacMillan R. L, Mustard J. F. 95-107 MacMillan of Canada; Toronto: 1961
    Search in Google Scholar
  • 3 Berman H. J, Tangen O, Ausprunk D, Collins H. Prostaglandin E1 inhibition of hamster platelets. Bibl. Anat. (In press.)
    PubMed
  • 4 Born G. V. R. Quantitative investigations into the aggregation of blood platelets. J. Physiol. (Lond.) 162: 67-68P 1962;
    PubMedSearch in Google Scholar
  • 5 Born G. V. R. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (Lond.) 194: 927-929 1962;
    PubMedSearch in Google Scholar
  • 6 Born G. V. R, Cross M. J. Effects of inorganic ions and of plasma proteins on the aggregation of blood platelets by adenosine diphosphate. J. Physiol. (Lond.) 170: 397-414 1964;
    CrossrefPubMedSearch in Google Scholar
  • 7 Born G. V. R, Honour A. J, Mitchell J. R. Inhibition by adenosine and by 2-chloroadenosine of the formation and embolization of platelet thrombi. Nature (Lond.) 202: 761-765 1964;
    CrossrefPubMedSearch in Google Scholar
  • 8 Gaarder A, Jonsen J, Laland S, Hellem A, Owren P. A. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature (Lond.) 192: 531-532 1961;
    CrossrefPubMedSearch in Google Scholar
  • 9 Haslam R. J. Biochemical aspects of platelet function. In: Plenary session papers. XII. Congress International Society of Hematology (New York). Ed. Jaffé, E.R; 198-214 1968
    Search in Google Scholar
  • 10 Haslam R. J. Role of adenosine diphosphate in the aggregation of human blood-platelets by thrombin and by fatty acids. Nature (Lond.) 202: 765-768 1964;
    CrossrefPubMedSearch in Google Scholar
  • 11 McKinnon E. L, Tangen O, Berman H. J. To be published.
    PubMed
  • 12 O’Brien J. R. Platelet aggregation II. Some results from a new method of study. J. clin. Path 15: 452-455 1962;
    PubMedSearch in Google Scholar
  • 13 O’Brien J. R, Heywood J. B, Heady J. A. The quantitation of platelet aggregation induced by four compounds: A study in relation to myocardial infarction. Thrombos. Diathes. haemorrh. (Stuttg.) 16: 752-767 1966;
    PubMedSearch in Google Scholar
  • 14 Saltzman E. W, Chambers D. A, Neri L. L. Incorporation of labelled nucleotides and aggregation of human blood platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 52-68 1966;
    PubMedSearch in Google Scholar
  • 15 Shoza L, Zucker M. B, Jerushalmy Z, Grant R. Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds and adenosine. Thrombos. Diathes. haemorrh. (Stuttg.) 18: 713-725 1967;
    PubMedSearch in Google Scholar
  • 16 Solum N. O, Stormorken H. Influence of fibrinogen on the aggregation of washed human blood platelets induced by adenosine diphosphate, thrombin, collagen and adrenaline. Scand. J. clin. Lab. Invest 17 (Suppl. 84) 170-182 196 1965;
    PubMedSearch in Google Scholar
  • 17 Tangen O, Berman H. J. To be published.
    PubMed
  • 18 Wintrobe M. M. Clinical Hematology. 356-357 Lea & Febiger; Philadelphia: 1961
    Search in Google Scholar