Aggregation of Fibrinogen and Its Degradation Products by Basic Proteins

G. J Stewart; S Niewiarowski

doi:10.1055/s-0038-1654330

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1971; 25(03): 566-579
DOI: 10.1055/s-0038-1654330

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Aggregation of Fibrinogen and Its Degradation Products by Basic Proteins

An Electron Microscope Study

G. J Stewart^*)

¹Department of Biological Sciences, Boston University and the Vascular Laboratory, Lemuel Shattuck Hospital, Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts

,

S Niewiarowski^**)

¹Department of Biological Sciences, Boston University and the Vascular Laboratory, Lemuel Shattuck Hospital, Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts

› Author Affiliations

Abstract

Summary

Fibrinogen and early fibrinogen degradation products (FDP) of plasmin digestion containing Fragment X^fP gave highly ordered polymers of limited branching with protamines and synthetic polylysine (molecular weight 175,000). Histones, polylysine of molecular weight 2,500, granulocyte cationic protein and platelet factor 4 formed amorphous precipitates with fibrinogen and early FDP. Fibrin degradation products (fdp) containing complexes of Fragment X° formed highly ordered polymers of extensive branching with these basic proteins.

The splitting off of fibrinopeptides from fibrinogen and Fragment X^fP was not essential for the formation of highly ordered polymers. However, in the presence of fibrinopeptides, high specificity of polymerizing agents such as basic protein is required. In the absence of fibrinopeptides, fibrin monomer or Fragment X° could polymerize after dissociation from complexes with other degradation products by any of the basic proteins.

It has been suggested that the polymerization of fibrinogen and its derivatives by basic proteins may be an important factor contributing to the formation and deposition of fibrin-like material in clinical conditions connected with the destruction of tissues (release of histones), damage of granulocytes (release of lysosomal cationic protein) and intravascular formation of platelet aggregates (release of platelet factor 4).

Full Text

References

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