Hydrolysis of Tosylarginine Methyl Ester by Russell’s Viper Venom

Robert H. Wagner; Robert A. Farrell

doi:10.1055/s-0038-1654409

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1959; 3(04): 548-553
DOI: 10.1055/s-0038-1654409

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Hydrolysis of Tosylarginine Methyl Ester by Russell’s Viper Venom

Robert H. Wagner^*

¹Department of Pathology, University of North Carolina, Chapel Hill, North Carolina USA

,

Robert A. Farrell^*

¹Department of Pathology, University of North Carolina, Chapel Hill, North Carolina USA

› Institutsangaben

Abstract

Summary

Russell’s viper venom (RVV) is capable of hydrolyzing TAME slowly. It is readily adsorbed by glass from weak solutions. The pH optimum for the hydrolysis was 8.7 to 9.0. Under the conditions specified, K_m was found to be 1.05 × 10^–2 M and V_max was 0.62 μM per min. per mg RVV. On a weight basis, RVV has a greater clot-accelerating activity than trypsin but less TAME hydrolyzing activity. It is suggested that there is a correlation between the clot-accelerating action of RVV and the hydrolysis of specific arginyl peptide bonds.

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Referenzen

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