The Separation of AHF from Fibrinogen. III^[*]

Summary

A chromatographic method is outlined permitting to obtain quantitatively the AHF, contained in 250 ml of human plasma, in a fibrinogen-free state.

^* This work was aided by a grant from the Organization for Pure Scientific Research in the Netherlands.

^** With technical assistance of Miss S. N. van Lare and Mrs. A. M. Schoeman-Leeuwangh.

• References

• (1) van Creveld S, Veder H. A, Pascha C. N. The separation of AHF from fibrinogen. II. Thromb. Diath. haem. 4: 211 1960;
• (2) Peterson E. A, Sober H. A. Chromatography of proteins. I. Cellulose ion-exchange adsorbents. J. Amer. chem. Sci. 78: 751 1956;
• (3) van Creveld S, Veder H. A, Pascha C. N, Kroeze W. F. The separation of AHF from fibrinogen. Thromb. Diath. haem. 3: 572 1959;
• (4) Sober H. A, Gutter F. J, Wijckhoff M. M, Peterson E. A. Chromatography of proteïns. II. Fractionations of serum-protein on anion-exchange cellulose. J. Amer. chem. Soc. 78: 756 1956;
• (5) Fahey J. L, McCoy P. F, Goulian M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B₁₂-binding protein, alkaline and acid phosphatases, ridioiodinated albumin and myeloma proteins. J. clin. Invest. 37: 272 1958;
• (6) Veder H. A, Pascha C. N. The relative affinities of a number of anions for ECTEOLA-cellulose. Biochim. biophys. Acta 47: 408 1961;
• (7) Seegers W. H, Landaburu R. H, Fenichel R. L. Isolation of platelet cofactor I from plasma and some properties of the preparations. Amer. J. Physiol. 190: 1 1957;