Quantitative Determination of the Amino Acids and other Ninhydrin Positive Substances in Normal Human Platelets

Summary

In normal human platelets twenty amino acids and additionally taurine, phosphoethanolamine and phosphoserine were determined on a quantitative basis, using elution chromatography on ion exchangers.

The highest molar share goes to taurine, glutamic acid, alanine, aspartic acid, proline and serine. The other amino acids can be found in free form only in a low concentration in platelets.

The connection of these results with other investigations on the metabolism is discussed.

• Literatur

• 1 Barkhan P, Silver M. J, O’Keife L. M. Blood platelets. Ed Johnson et al. 303 Little, Brown & Cie; Boston: 1961
• 2 Barkhan P, Silver M. J, da Costa P. B, Tocantins L. M. Nature 182: 1031 1958;
• 3 Eggstein M, Gross R. Verh. dtsch. Ges. inn. Med. 66: 981 1961;
• 4 Eggstein M. Personal communication. 1961
• 5 Gerok W. Klin. Wschr. 1960; 1212
• 6 Gerok W, Gross R. Thromb. Diath. haem. 3: 654 1959;
• 7 Gross R. Blood Platelets. Ed Johnson 319 Little, Brown & Cie; Boston: 1961
• 8 Hannig K. Clin. chim. Acta 4: 51 1959;
• 9 Hino A. Toho Univ. Report 2: 601 1960;
• 10 Kofrany E. Hoppe-Seylers Z. physiol. Chem. 299: 129 1955;
• 11 Marens A. J, Spaet Th. H. J. clin. Invest. 37: 1836 1958;
• 12 Marens A. J, Ullman H. L, Wofman M. Lipid Res. 1: 179 1960;
• 13 Mauri C, Torell M, Gross G. Biol. Soc. Ital. Biol. Sper. 34: 152 1958;
• 14 McMenamy R. H, Lund C. C. Biochim. biophys. Acta 45: 603 1960;
• 15 Moore S, Spackman D. H, Stein W. H. An. Chem. 30: 1185 1958;
• 16 Moore S, Stein W. H. J. biol. Chem. 192: 663 1951;
• 17 O’Brien J. R. J. clin. Path. 9: 47 1956;
• 18 O’Brien J. R. Nature 181: 420 1958;
• 19 Spackman D. H, Stein W. H, Moore S. An. Chem. 30: 1190 1958;
• 20 Troup S. B, Reed C. F, Marinetti G. W, Swisher S. N. Blood platelets. Ed Johnson Monto R. W, Rebuck J. W, Horn R. C. 265 Little, Brown & Cie; Boston: 1961