Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1963; 09(02): 248-263DOI: 10.1055/s-0038-1654980 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Aggregation of Rabbit Blood Platelets Produced in Vitro by Saline “Extract” of Tendons[*] Authors Author Affiliations Torstein Hovig ** 1 Department of Physiology, Veterinary College of Norway, Oslo Recommend Article Abstract Buy Article(opens in new window) Summary Rabbit blood platelet aggregates were produced in vitro by addition of rabbit tendon “extract” to citrated platelet-rich plasma. The aggregating activity was not due to presence of adenosine diphosphate in the “extracts” and was unrelated to blood coagulation. The aggregating principle was destroyed by heating of the “extract” to 60° C for 15 minutes or by incubation with collagenase for 1 hour at 37° C. The aggregating effect was associated with particles which were sedi- mented by centrifugation for 30 minutes at 10,000 G. By means of electron microscopy the particles were identified as cross-striated collagen fibrils. Full Text References References 1 Apitz K. Die Bedeutung der Gerinnung und Thrombose für die Blutstillung. Virchows Arch. path. Anat. 308: 540-614 1942; 2 Bjornesjo K. B. Staining of protein-bound serum polysaccharides in electrophoresis strips. Scand. J. clin. Lab. Invest. 07: 153-159 1955; 3 Blackstad T. W, Vegge T. On the ultrastructure of vitreous body filaments. Acta ophthal. (Kbh) 39: 37-53 1961; 4 Bounameaux Y. L’accolement des plaquettes aux fibres sous—endothéliales. Thrombos. Diathes. haemorrh. (Stuttg) 06: 504-516 1961; 5 Gross J, Highberger J. H, Schmitt F. O. Collagen structures considered as states of aggregation of a kinetic unit. The tropocollagen particle. Proc. nat. Acad. Sci. (Wash) 40: 679-688 1954; 6 Gross J, Highberger J. H, Schmitt F. O. Extraction of collagen from connective tissue by neutral salt solutions. Proc. nat. Acad. Sci. (Wash) 41: 1-7 1955; 7 Gaarder A, Jonson J, Laland S, Hellem A, Owren P. A. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature 192: 531-532 1961; 8 Harrington W. F, von Hippel P. H. The structure of collagen and gelatin. Advanc. Protein Chem. 16: 76 1961; 9 Hellem A. J. The adhesiveness of human blood platelets in vitro. Scand. J. clin. Lab Invest. 12 (Suppl. 51): 1-117 1960; 10 Hjort P. F. Intermediate reactions in the coagulation of blood with tissue thromboplastin. Scand. J. clin. Lab. Invest. 02 (Suppl. 27): 1-183 1957; 11 Hovig T. The ultrastructure of rabbit blood platelet aggregates. Thrombos. Diathes. haemorrh. (Stuttg) 08: 455-471 1962; 12 Hugues J. Contribution a l’étude des facteurs vasculaires et sanguins dans l’hémostase spontanée. Arch. int. Physiol. 41: 565-711 1953; 13 Hugues J. Accolement des plaquettes au collagéne. C. R. Soc. Biol. (Paris) 154: 866-868 1960; 14 Kjærheim Ä, Hovig T. The ultrastructure of haemostatic blood platelet plugs in rabbit mesenterium. Thrombos. Diathes. haemorrh. (Stuttg) 07: 1-15 1962; 15 Leach A. A. Notes on a modification of the Neuman & Logan method for the determination of the hydroxyproline. Biochem. J. 74: 70-71 1960; 16 O’Brien J. R. Platelet aggregation. J. clin. Path. 15: 446-455 1962; 17 Palade G. E. A study of fixation for electron microscopy. J. exp. Med. 95: 285-298 1952; 18 Randall J. T, Booth F, Burger R. E, Jackson SFitton, Kelly F. C. Observations on native and precipitated collagen. Symp. Soc. exp. Biol. 09: 127-147 1955; 19 Schmitt F. O. Macromolecular interaction patterns in biological systems. Proc. Amer, phil. Soc. 100: 476-486 1956; 20 Zucker M. B. Platelet agglutination and vasoconstriction as factors in spontaneous haemostasis in normal, thrombocytopenic, heparinized and hypoprothrombinémie rats. Amer. J. Physiol. 248: 275-288 1947; 21 Zucker M. B, Borrelli J. Platelet clumping produced by connective tissue suspensions and by collagen. Proc. Soc. exp. Biol. (N. Y) 109: 779-787 1962;
