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Thromb Haemost 1963; 09(02): 446-458
DOI: 10.1055/s-0038-1654997
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Interaction of Heparin with Fibrinolysis[*]

Rudolf Holemans M.D   **
1   The Joseph Stanton Memorial Research Laboratories, St. Elizabeth’s Hospital and Tuft’s University Medical School, Boston, Mass., USA
,
Dionysios Adamis M.D   ***
1   The Joseph Stanton Memorial Research Laboratories, St. Elizabeth’s Hospital and Tuft’s University Medical School, Boston, Mass., USA
,
James F Horace M.A   ****
1   The Joseph Stanton Memorial Research Laboratories, St. Elizabeth’s Hospital and Tuft’s University Medical School, Boston, Mass., USA
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Publikationsdatum:
21. Juni 2018 (online)

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Summary

Heparin in high concentration inhibits the fibrinolysis of human plasma clots or bovine fibrin by fibrinolytic agents which produce plasminogen activation. Heparin has no effect on the fibrinolytic activity of plasmin or Aspergillus protease.

In order to produce inhibition of plasminogen activation heparin requires the presence of a co-factor which is present in citrated human plasma but absent from its euglobulin fraction.

In none of the concentrations tested has heparin an enhancing effect on fibrinolysis.

* Supported by grant H-5698 from the National Institute of Health.


** Fellow in Hematology at Tuft’s University Medical School, 1960-1961. Fulbright Research Scholar on leave of absence from the University of Louvain, Belgium.


*** Acting Director of Research, St. Elizabeth’s Hospital.


**** Biologist, M.A., Boston University.


 

  • References

  • 1 Halse T. Ergänzender Beitrag zur Aktivierung der Fibrinolyse des nativen Plasma- koagulums durch Heparin. Arch. int. Phärmacodyn. 86: 168-176 1951;
    PubMedGoogle Scholar
  • 2 Schmidhauser-Kopp M, Eichenberger E. Über den Einfluß von Thrombin und Heparin auf die Fibrinolyse. Experientia (Basel) 08: 354-355 1952;
    CrossrefPubMedGoogle Scholar
  • 3 Vinazzer H. Untersuchungen über die fibrinolytische Wirkung des Heparins. Wien. 2. inn. Med. 32: 167-173 1951;
    PubMedGoogle Scholar
  • 4 Buluk K, Januszko J. Heparyna a Fibrynoliza. Pat. pol. 08: 107-114 1957;
    PubMedGoogle Scholar
  • 5 Paly S, Kline D. L. The spontaneous proteolytic activity of dog blood. Yale J. Biol. Med. 26. 486-490 (1953-1954
    PubMedGoogle Scholar
  • 6 Astrup T, Crookston J, MacIntyre A. Proteolytic enzymes in blood. Acta physiol, scand. 21: 238-249 1950;
    CrossrefPubMedGoogle Scholar
  • 7 Astrup T, ALkjaersig N. Classification of proteolytic enzymes by means of their inhibitors. Nature 169: 314-316 1952;
    CrossrefPubMedGoogle Scholar
  • 8 Von Kaulla K. N, McDonald T. S. The effect of heparin on components of the human fibrinolytic system. Blood 13: 811-821 1958;
    PubMedGoogle Scholar
  • 9 Von Kaulla K. N. Safe dissolution of pathologic human fibrin deposits. A therapeutic challenge. Pharm. Acta Helv. 33: 727-733 1958;
    PubMedGoogle Scholar
  • 10 Von Kaulla K. N, Swan H. Clotting deviations in man during cardiac bypass: fibrinolysis and circulating anticoagulant. J. thorac. Surg. 36: 519-533 1958;
    PubMedGoogle Scholar
  • 11 Owren P. A. The Coagulation of blood. Investigations on a new clotting factor. Acta med. scand. (Suppl) 194: 1-327 1947;
    PubMedGoogle Scholar
  • 12 Milstone H. A factor in normal human blood which participates in streptococcal fibrinolysis. J. Immunol. 42: 109-116 1941;
    PubMedGoogle Scholar
  • 13 Clement W, McNicol G. R. The preparation and use of Iodine131-tagged fibrinogen to demonstrate fibrinolysis. J. clin. Path. 12: 544-550 1959;
    CrossrefPubMedGoogle Scholar
  • 14 Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. 40: 346-351 1952;
    CrossrefPubMedGoogle Scholar
  • 15 Müllertz S, Lassen M. An activator system in blood indispensable for formation of plasmin by streptokinase. Proc. Soc. exp. Biol. (N. Y) 82: 264-268 1953;
    CrossrefPubMedGoogle Scholar
  • 16 Kline D. L. Proceedings of the Conference on thrombolytic Agents, April 8-9, 1960, Chicago (111.)». Ed. Roberts H. R, Geratz J. D. 253-256 University of North Carolina. Chapel Hill (N. C): 1960
    PubMedGoogle Scholar
  • 17 Niewiarowski S, Latallo Z. Comparative studies on the fibrinolytic system of sera of various vertebrates. Thrombos. Diathes. haemorrh. (Stuttg) 03: 404-417 1959;
    PubMedGoogle Scholar
  • 18 Marin H. M, Stefanini M, Soardi F, Mueller L. Fibrinolysis V. The thrombolytic and anticoagulant activity of mold fibrinolysin (Aspergillin O) in vivo. J. Lab. clin. Med. 58: 47-59 1961;
    PubMedGoogle Scholar
  • 19 Crewther W. G, Lennox F. G. Preparation of crystals containing protease from Aspergillus Oryzae. Nature 165: 680 1950;
    CrossrefPubMedGoogle Scholar
  • 20 Schultze F. L. E, Schwick G. Uber den Mechanismus der Thrombinbildung im isolierten System. Hoppe-Seylers Z. physiol. Chem. 289: 26-43 1952;
    PubMedGoogle Scholar
  • 21 Giacomazzi G. L’influenza delPeparina sulla fibrinolisi. II Policlinico. 62: 226-239 1955;
    PubMedGoogle Scholar
  • 22 Bauer G. Early diagnosis of venous thrombosis by means of venography and abortive treatment with heparin. Acta med. scand. 107: 136-147 1941;
    PubMedGoogle Scholar
  • 23 Loewe L, Hirsch E, Grayzel D. M. The action of heparin on experimental venous thrombosis. Surgery 22: 746-760 1947;
    PubMedGoogle Scholar
  • 24 Sandritter W, Huppert M, Schlütter G. Zur Frage der Fibrinolyse an experimentellen Gerinnungs- und Abscheidungsthromben. Klin. Wschr. 1958; 651-655.
    PubMedGoogle Scholar
  • 25 Deutsch E. Blutgerinnungsfaktoren. 181 Textbook. Deuticke; Vienna: 1955
    Google Scholar
  • 26 Godal H. C. The influence of heparin on the fibrin clot. Scand. J. clin. Lab. Invest 13: 306-313 1960;
    PubMedGoogle Scholar
  • 27 Alkjaersig N, Fletcher A. P, Sherry S. Epsilon aminocaproic acid: an inhibitor of plasminogen activation. J. biol. Chem. 239: 832-837 1959;
    PubMedGoogle Scholar
  • 28 Gross R, Holemans R. Fragen der Blutgerinnung bei extrakorporalem Kreislauf mit der Herz-Lungen-Maschine. Klin. Wschr. 1961; 165-173.
    PubMedGoogle Scholar
  • 29 Holemans R, Adamis D, Horace J. Heparine et fibrinolyse. Experientia (Basel) 18: 377-378 1962;
    CrossrefPubMedGoogle Scholar