Download PDF
Thromb Haemost 1966; 16(01/02): 265-276
DOI: 10.1055/s-0038-1655561
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Influence of Peanut Extract on Bleeding Time

V. L Frampton   *
,
Louise S. Lee   *
,
Nelle J. Morris   *
,
H. B Boudreaux   **
Further Information

Publication History

Publication Date:
26 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

The bleeding time of transected arterioles in the cheek pouch of hamsters ingesting an alcohol extract of defatted peanuts was shortened to about 3/4 that of untreated control animals. Solvent separation and chromatographic methods were used in producing a concentrate representing 0.0025% of the original peanut kernels. The concentrate shortened bleeding time, caused vasoconstriction of transected arterioles after hemostasis and induced strong contraction of isolated duodenum preparations. The contraction response of duodenum was used as a guide for separation methods. The activity on duodenum was high at concentrations of 10-7 g/ml of Tyrode’s solution. Preliminary determinations indicate that the active factor might be a lactone but impurities in the best concentrates preclude its positive identification. It is soluble in water, sparingly soluble in ethanol and methanol, very slightly soluble in boiling acetone, insoluble in butanol, chloroform, diethyl ether, 2,2-dimethoxypropane, pyridine and petroleum ether. A possible role in hemostasis is discussed.

* Address : Southern Utilization and Research Development Division, Agricultural Research Service, U. S. Dept. Agric, New Orleans, Louisiana, U. S. A. 70119.


** Address: Department of Entomology, Louisiana State University, Baton Rouge, Louisiana, U. S. A. 70803.


 

  • References

  • 1 Abe T, Sato A, Kazama M, Matsumara T. Effect of E-aminocaproic acid in hemophilia. Lancet II: 405 1962;
    PubMedSearch in Google Scholar
  • 2 Astrup T, Brakman P, Ollendorf P, Rasmussen J. Haemostasis in haemophilia in relation to the haemostatic balance in the normal organism and the effect of peanuts. Thrombos. Diathes. haemorrh. (Stuttg.) 5: 329-331 1960;
    PubMedSearch in Google Scholar
  • 3 Astrup T, Brakman P, Sjolin K. E. Haemophilia and the protease inhibitor in peanuts. Nature (Lond.) 194: 981 1962;
    CrossrefPubMedSearch in Google Scholar
  • 4 Barkham P. Haematura in a haemophiliac treated with E. aminocaproic acid. Lancet II: 647 1964;
    PubMedSearch in Google Scholar
  • 5 Bisordi M. Peanut flour in haemophilia. Lancet II: 476 1964;
    PubMedSearch in Google Scholar
  • 6 Boudreaux H. B, Frampton V. L. A peanut factor for haemostasis in haemophilia. Nature (Lond.) 185: 469-470 1960;
    CrossrefPubMedSearch in Google Scholar
  • 7 Boudreaux H. B, Boudreaux R. M, Brandon M. B, Frampton V. L, Lee L. S. Bioassay of a haemostatic factor from peanuts. Arch. Biochem 89: 276-280 1960;
    CrossrefPubMedSearch in Google Scholar
  • 8 Brakman P, Sjolin K. E, Astrup T. Is there a haemostatic effect of peanuts in haemo-philoid disorders?. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 442-456 1962;
    PubMedSearch in Google Scholar
  • 9 Brüster H. Untersuchungen über die Wirkung einzelner Erdnußfraktionen auf die Blutstillung bei der Hämophilie. Klin. Wschr 21: 1145 1961;
    PubMedSearch in Google Scholar
  • 10 Brüster H. Gerinnungsphysiologische und papierchromatographische Untersuchungen bei der Hämophilie wirksamen Erdnußfraktionen. Z. Kinderheilk 86: 462-468 1962;
    CrossrefPubMedSearch in Google Scholar
  • 11 Gepelák V. Zur Therapie der Blutungszustände mit Erdnußextrakten. Zges. inn. Med 20: 317-320 1963;
    PubMedSearch in Google Scholar
  • 12 Gordon A. M, McNicol G. P, Dubber A. H. C, McDonald G. A, Douglas A. S. Clinical trial of epsilon-aminocaproic acid in severe haemophilia. Brit. Med. J I: 1632-1635 1965;
    PubMedSearch in Google Scholar
  • 13 Jackson B, Owen W, Boudreaux H. B. Pharmacological and chemical chracteristics of peanut extracts effective in hemostasis. Thrombos. Diathes. haemorrh. (Stuttg.) 16: 257 1966;
    PubMedSearch in Google Scholar
  • 14 Jørgensen L, Borchgrevink C. F. The haemostatic mechanism in patients with haemorrhagic diseases. Acta path. Microbiol, scand 60: 55-82 1964;
    CrossrefPubMedSearch in Google Scholar
  • 15 Lee L. S, Morris N. J. Isolation and identification of pinitol from peanut flour. J. Agr. Food Chem 11: 321-322 1963;
    CrossrefPubMedSearch in Google Scholar
  • 16 Mainwaring D, Keidan S. E. Peanut flour in haemophilia. Lancet II: 647 1964;
    PubMedSearch in Google Scholar
  • 17 Morris N. J, Lee L. S. Isolation and identification of a sterol glucoside from peanut flour. J. Agr. Food Chem 9: 401-403 1961;
    CrossrefPubMedSearch in Google Scholar
  • 18 Ried W. O, Lucas O. N, Francisco J, Geisler P. H, Ersley A. J. The use of epsilon-aminocaproic acid in the management of dental extractions in the hemophiliac. Amer. J. med. Sci 248: 184-188 1964;
    CrossrefPubMedSearch in Google Scholar
  • 19 Roskam J. Erreurs anciennes et prespectives nouvelles dans l’étude de l’adhésion, de l’agrégation et de la metamorphose visqueuse des plaquettes, facteurs d’hémostase. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 253-266 1964;
    PubMedSearch in Google Scholar
  • 20 Tsevrenis H, Mandalaki T. Haematura in a haemophiliac treated with E-aminocaproic acid. Lancet I: 610 1965;
    PubMedSearch in Google Scholar
  • 21 van Creveld S, Mochtar I. A, Koppe J. G. Haemophilia and therapy with peanuts, II. Ann. Paediat 202: 1-5 1964;
    PubMedSearch in Google Scholar