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Thromb Haemost 1966; 16(01/02): 038-050
DOI: 10.1055/s-0038-1655625
Originalarbeiten — Original Articles — Travaux Originaux
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Determination of Plasminogen in Clots and Thrombi[*]

Ulla Hedner
1   Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden
,
Inga Marie Nilsson
1   Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden
,
B Robertson
1   Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden
› Author Affiliations
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Publication Date:
26 June 2018 (online)

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Summary

The plasminogen content was determined by a casein method in plasma and serum from 20 normal volunteers. The mean plasminogen content was found to be 10.1 ACU (the arbitrary caseinolytic unit defined in such a way that using a 3% casein solution and a digestion time of 20 min. at 37°C, 10 ACU gave an extinction of 0.300). No difference between serum and plasma regarding the plasminogen content was found.

Plasminogen was determined in drained and drained plus washed clots prepared from 2 ml plasma. The highest values found in the drained clots were 0.9 ACU/clot and 0.2 ACU/clot in the drained plus washed clots.

Plasminogen was also determined in drained and drained plus washed clots prepared from plasma with added purified plasminogen. The plasminogen was recovered in the washing fluid. According to these tests, then, purified added plasminogen is washed out of the clots.

The plasminogen content of 20 thrombi obtained post mortem was also determined. The mean value was found to be 0.7 ACU/cm thrombus. Judging from our results, the “intrinsic clot lysis theory” is not the main mechanism of clot dissolution.

* This investigation was supported by grants from the Swedish Medical Research Council, the Medical Faculty of Lund and from the National Institutes of Health, Public Health Service (Research Grant HE 07066).


 

  • References

  • 1 Alkjaersig N, Fletcher A. P, Sherry S. The mechanism of clot dissolution by plasmin. J. clin. Invest 38: 1086 1959;
    CrossrefPubMedSearch in Google Scholar
  • 2 Ambrus J. L, Markus G. Plasmin-antiplasmin complex as reservoir of fibrinolytic enzyme. Amer. J. Physiol 199: 491 1960;
    PubMedSearch in Google Scholar
  • 3 Dudok de Wit G. The role of plasminogen in fibrinolysis. Vox Sang (Basel) 7: 526 1962;
    CrossrefPubMedSearch in Google Scholar
  • 4 Fantl P. Plasminogen activity of plasma and serum. Science 135: 787 1962;
    CrossrefPubMedSearch in Google Scholar
  • 5 Hedner U, Nilsson I. M. Determination of plasminogen in human plasma by a casein method. Thrombos. Diathes. haemorrh. (Stuttg.) 14: 545 1965;
    PubMedSearch in Google Scholar
  • 6 Kline D. L. Purification and crystallization of plasminogen (Profibrinolysin). J. biol. Chem 204: 949 1953;
    PubMedSearch in Google Scholar
  • 7 Müllertz S. Formation and properties of the activator of plasminogen and of human and bovin plasmin. Biochem. J 61: 424 1955;
    CrossrefPubMedSearch in Google Scholar
  • 8 Nilsson I. M, Blombäck M, Francken I. v. On an inherited autosomal hemorrhagic diathesis with antihemophilic globulni (AHG) deficiency and prolonged bleeding time. Acta med. scand 159: 35 1957;
    PubMedSearch in Google Scholar
  • 9 Nilsson I. M, Blombäck M, Ramgren O. Hemophilia in Sweden. Coagulation studies. Acta med. scand 170: 665 1961;
    PubMedSearch in Google Scholar
  • 10 Nilsson I. M, Olow B. Determination of fibrinogen and fibrinogenolytic activity. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 297 1962;
    PubMedSearch in Google Scholar
  • 11 Nilsson I. M, Olow B. Fibrinolysis induced by streptokinase in man. Acta chir. scand 123: 247 1962;
    PubMedSearch in Google Scholar
  • 12 Ogston D, Ogston G. M, Ftdlerton H. W. The plasminogen content of thrombi. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 220 1966;
    PubMedSearch in Google Scholar
  • 13 Oncley J. L, Melin M, Richert D. A, Cameron J. W, Gross Jr. P. M. The separation of the antibodies, isoagglutinins, prothrombin plasminogen and β1 -lipoprotein into subfraction of human plasma. J. amer. chem. Soc 71: 541 1949;
    CrossrefPubMedSearch in Google Scholar
  • 14 Paraskevas M, Nilsson I. M, Martinsson G. A method for determining serum inhibitors of plasminogen activation. Scand. J. clin. Lab. Invest 14: 138 1962;
    CrossrefPubMedSearch in Google Scholar
  • 15 Sawyer W. D, Alkjaersig N, Fletcher A. P, Sherry S. Thrombolytic therapy. Basic and therapeutic considerations. A. M. A. Arch, intern. Med 107: 274 1961;
    CrossrefPubMedSearch in Google Scholar
  • 16 Wallén P. Studies on the purification of human plasminogen. I. The preparation of a partially purified human plasminogen with a low spontaneous activity. Arkiv Kemi 19: 451 1962;
    PubMedSearch in Google Scholar
  • 17 Wallen P. Studies on the purification of human plasminogen. II. Further purification of human plasminogen on cellulose ion exchanges and by means of gel filtration through sepha-dex. Arkiv Kemi 19: 469 1962;
    PubMedSearch in Google Scholar
  • 18 Gottlob R, Blümel G. Über den Nachweis der Plasminogenverarmung in retrahierten Vollblutgerinnseln und über deren Bedeutung für die Lysierbarkeit mit Streptokinase. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 570 1966;
    PubMedSearch in Google Scholar