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Thromb Haemost 1966; 16(01/02): 122-133
DOI: 10.1055/s-0038-1655632
DOI: 10.1055/s-0038-1655632
Originalarbeiten — Original Articles — Travaux Originaux
The Effect of Sulphydryl and Enzyme Inhibitors on Platelet Aggregation in Vitro
Further Information
Publication History
Publication Date:
26 June 2018 (online)
Summary
The glycolytic enzyme poisons potassium cyanide, sodium fluoride, and monofluoroacetate do not inhibit platelet clumping. The sulphydryl inhibitors, n-ethyl maleimide and p-hydroxymercuribenzoate, inhibit platelet aggregation produced by ADP, ATP, nor-adrenaline, 5HT, thrombin and connective tissue extract. This inhibitory activity is counteracted by 1-cysteine. These results suggest that platelet thiol groups are involved in the platelet adhesion occurring during aggregation.
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References
- 1 Born G. V. R. Quantitative investigations into the aggregation of blood platelets. J. Physiol. (Lond.) 162: 67P 1962;
- 2 Von Braunsteiner H, Pakesch F, Sailer F. S. Über morphologische Veränderungen an den Thrombozyten durch Fermenthemmer und ihren Zusammenhang mit der Thrombosthenie. Actahaemat. (Basel) 24: 311 1960;
- 3 Emmons P. R, Mitchell J. R. A. Postoperative changes in platelet clumping activity. Lancet 7: 71 1965;
- 4 Dixon M, Webb E. G. Enzymes. Longmans; London: 1958
- 5 Fantl P. Thiol groups of blood platelets in relation to clot retraction. Nature (Lond.) 198: 95 1961;
- 6 Friedmann E, Marrian D. H, Simon-Reuss I. Antimitotic action of maleimide and related substances. Brit. J. Pharmacol 4: 105 1949;
- 7 Gaarder A, Laland S. Hypothesis for the aggregation of platelets by nucleotides. Nature (Lond.) 202: 909 1964;
- 8 Garvin J. E. Factors affecting the adhesiveness of human leucocytes and platelets in vitro. J. exp. Med 114: 55 1961;
- 9 Grette K. Studies on the mechanism of thrombin catalysed haemostatic reactions in blood platelets. Acta. phys. scand. Suppl. 195 1962
- 10 Koppel J. L, Novak L. V, Olwin J. H. Relation between platelet integrity and sulphydryl groups. Proc. Soc. exp. Biol. (N. Y.) 100: 227 1959;
- 11 Mitchell J. R. A, Sharp A. A. Platelet clumping in vitro. Brit. J. Haemat 10: 78 1964;
- 12 Morgan E. J, Friedmann E. C. Interaction of maleic acid with thiol compounds. Biochem. J 32: 733 1938;
- 13 O’Brien J. R. Platelet Aggregation. Part 1. Some effects of the adenosine phosphates, thrombin and cocaine upon platelet adhesiveness. J. clin. Path 15: 446 1962;
- 14 Ollgaard E. Macroscopic studies of platelet aggregation. Nature of an aggregating factor in red blood cells and platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 6: 86 1961;
- 15 Robinson Jr. G. W, Mason R. G, Wagner R. H. Effect of sulphydryl inhibitors on platelet agglutinability. Proc. Soc. exp. Biol. (N. Y.) 113: 857 1963;
- 16 Skcdhegg B. A, Hellem A. J, Ödegaard A. E. Investigations on adenosine diphosphate (ADP) induced platelet adhesiveness in vitro. Part II. Studies on the mechanism. Thrombos. Diathes. haemorrh. (Stuttg.) 11: 305 1964;
- 17 Zucker M. B, Borrelli J. Platelet clumping produced by connective tissue suspensions and by collagen. Proc. Soc. exp. Biol. (N.Y.) 109: 779 1962;