The Effect of Sulphydryl and Enzyme Inhibitors on Platelet Aggregation in Vitro

M. J. G Harrison; P. R Emmons; J. R. A Mitchell

doi:10.1055/s-0038-1655632

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1966; 16(01/02): 122-133
DOI: 10.1055/s-0038-1655632

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Effect of Sulphydryl and Enzyme Inhibitors on Platelet Aggregation in Vitro

M. J. G Harrison

¹ Department of the Regius Professor of Medicine, Radcliffe Infirmary, Oxford

,

P. R Emmons

¹ Department of the Regius Professor of Medicine, Radcliffe Infirmary, Oxford

,

J. R. A Mitchell

¹ Department of the Regius Professor of Medicine, Radcliffe Infirmary, Oxford

› Author Affiliations

Abstract

Summary

The glycolytic enzyme poisons potassium cyanide, sodium fluoride, and monofluoroacetate do not inhibit platelet clumping. The sulphydryl inhibitors, n-ethyl maleimide and p-hydroxymercuribenzoate, inhibit platelet aggregation produced by ADP, ATP, nor-adrenaline, 5HT, thrombin and connective tissue extract. This inhibitory activity is counteracted by 1-cysteine. These results suggest that platelet thiol groups are involved in the platelet adhesion occurring during aggregation.

Full Text

References

References
1 Born G. V. R. Quantitative investigations into the aggregation of blood platelets. J. Physiol. (Lond.) 162: 67P 1962;
2 Von Braunsteiner H, Pakesch F, Sailer F. S. Über morphologische Veränderungen an den Thrombozyten durch Fermenthemmer und ihren Zusammenhang mit der Thrombosthenie. Actahaemat. (Basel) 24: 311 1960;
3 Emmons P. R, Mitchell J. R. A. Postoperative changes in platelet clumping activity. Lancet 7: 71 1965;
4 Dixon M, Webb E. G. Enzymes. Longmans; London: 1958
5 Fantl P. Thiol groups of blood platelets in relation to clot retraction. Nature (Lond.) 198: 95 1961;
6 Friedmann E, Marrian D. H, Simon-Reuss I. Antimitotic action of maleimide and related substances. Brit. J. Pharmacol 4: 105 1949;
7 Gaarder A, Laland S. Hypothesis for the aggregation of platelets by nucleotides. Nature (Lond.) 202: 909 1964;
8 Garvin J. E. Factors affecting the adhesiveness of human leucocytes and platelets in vitro. J. exp. Med 114: 55 1961;
9 Grette K. Studies on the mechanism of thrombin catalysed haemostatic reactions in blood platelets. Acta. phys. scand. Suppl. 195 1962
10 Koppel J. L, Novak L. V, Olwin J. H. Relation between platelet integrity and sulphydryl groups. Proc. Soc. exp. Biol. (N. Y.) 100: 227 1959;
11 Mitchell J. R. A, Sharp A. A. Platelet clumping in vitro. Brit. J. Haemat 10: 78 1964;
12 Morgan E. J, Friedmann E. C. Interaction of maleic acid with thiol compounds. Biochem. J 32: 733 1938;
13 O’Brien J. R. Platelet Aggregation. Part 1. Some effects of the adenosine phosphates, thrombin and cocaine upon platelet adhesiveness. J. clin. Path 15: 446 1962;
14 Ollgaard E. Macroscopic studies of platelet aggregation. Nature of an aggregating factor in red blood cells and platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 6: 86 1961;
15 Robinson Jr. G. W, Mason R. G, Wagner R. H. Effect of sulphydryl inhibitors on platelet agglutinability. Proc. Soc. exp. Biol. (N. Y.) 113: 857 1963;
16 Skcdhegg B. A, Hellem A. J, Ödegaard A. E. Investigations on adenosine diphosphate (ADP) induced platelet adhesiveness in vitro. Part II. Studies on the mechanism. Thrombos. Diathes. haemorrh. (Stuttg.) 11: 305 1964;
17 Zucker M. B, Borrelli J. Platelet clumping produced by connective tissue suspensions and by collagen. Proc. Soc. exp. Biol. (N.Y.) 109: 779 1962;