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Thromb Haemost 1997; 77(01): 048-052
DOI: 10.1055/s-0038-1655905
Clinical Studies
Schattauer GmbH Stuttgart

Thrombin Activity Associated with Indwelling Central Venous Catheters

Charles W Francis
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, New York, USA
,
Andrew H Felcher
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, New York, USA
,
Janice White
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, New York, USA
,
Julie V Braaten
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, New York, USA
,
Rachel Goss
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, New York, USA
› Author Affiliations
Further Information

Publication History

Received 05 March 1996

Accepted after resubmisssion 11 September 1996

Publication Date:
11 July 2018 (online)

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Summary

Thrombotic complications are frequent with indwelling central venous catheters and result in catheter dysfunction, vascular obstruction and may also contribute to catheter-associated infections. The pathogenesis of catheter thrombosis is not well characterized but may involve vessel damage, local stasis and catheter-associated thrombin formation. We have, therefore, measured the thrombin activity associated with central venous catheters removed from patients and have also determined the ability of hirudin to inactivate catheter-associated thrombin. We obtained 48 catheters from 46 patients and removed 1 cm portions for study. These were taken from the distal end, 5 cm proximal, and 15 cm proximal from the end. Following washing, thrombin activity was measured with a chromogenic assay. Thrombin was associated with 40 of 48 catheters and with 100 of 144 segments with a mean activity of 132 ± 27 μU/cm with a range of 0 to 2,160 μU/cm. Incubation in hirudin reduced the activity from a mean of 122 ± 33 μU/cm to 18 ± 6 μU/cm (p <.001). Scanning electron microscopy of selected catheters showed that some had areas of fibrin deposition which was not apparent visually. The findings indicate that indwelling central venous catheters frequently have associated thrombin activity which can be inhibited by a direct-acting thrombin inhibitor such as hirudin.

 

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