Anticoagulant Properties of the Vascular Endothelium

Thomas Bombeli; Michele Mueller; Andre Haeberli

doi:10.1055/s-0038-1655981

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1997; 77(03): 408-423
DOI: 10.1055/s-0038-1655981

Review Article

Schattauer GmbH Stuttgart

Anticoagulant Properties of the Vascular Endothelium

Authors

Thomas Bombeli

The Laboratory for Thrombosis Research, Department of Medicine, University Hospital of Bern, Switzerland
Michele Mueller

The Laboratory for Thrombosis Research, Department of Medicine, University Hospital of Bern, Switzerland
Andre Haeberli

The Laboratory for Thrombosis Research, Department of Medicine, University Hospital of Bern, Switzerland

Abstract

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References

References
1 Beresford CH, Owen MC. Antithrombin III. Int J Biochem 1990; 22: 0121-0128
2 Rao LVM, Rapaport SI, Hoang AD. Binding of factor VIIa to tissue factor permits rapid antithrombin III/heparin inhibition of factor VIIa. Blood 1993; 81: 2600-2607
3 Damus PS, Hicks M, Rosenberg RD. Anticoagulant action of heparin. Nature 1973; 246: 0355-0357
4 Teien AN, Abildgaard U, Hook M. The anticoagulant effect of heparan sulfate and dermatan sulfate. Thromb Res 1976; 8: 0859-0867
5 Hatton MWC, Berry LR, Regoeczi E. Inhibition of thrombin by antithrombin III in the presence of certain glycosaminoglycans found in the mammalian aorta. Thromb Res 1978; 13: 0655-0670
6 Marcum JA, Rosenberg RD. Anticoagulantly active heparin-like molecules from vascular tissue. Biochemistry 1984; 23: 1730-1737
7 Marcum JA, Fritze L, Galli SJ, Karp G, Rosenberg RD. Microvascular heparin-like species with anticoagulant activity. Am J Physiol 1983; 245: H725-H733
8 Colburn P, Buonassisi V. Anticlotting activity of endothelial cell cultures and heparan sulfate proteoglycans. Biochem Biophys Res Commun 1982; 104: 0220-0227
9 Marcum JA, Rosenberg RD. Heparin-like molecules with anticoagulant activity are synthesized by cultured endothelial cells. Biochem Biophys Res Commun 1985; 126: 0365-0372
10 Marcum JA, Atha DH, Fritze LMS, Nawroth P, Stern D, Rosenberg RD. Cloned bovine aortic endothelial cells synthesize anticoagulantly active heparan sulfate proteoglycan. J Biol Chem 1986; 261: 7507-7517
11 Nader HB, Dietrich CP, Buonassisi V, Colburn P. Heparin sequences in the heparan sulfate chains of an endothelial cell proteoglycan. Proc Natl Acad Sci USA 1987; 84: 3565-3569
12 Kojima T, Leone CW, Marchildon GA, Marcum JA, Rosenberg RD. Isolation and characterization of heparan sulfate proteoglycans produced by cloned rat microvascular endothelial cells. J Biol Chem 1992; 267: 4859-4869
13 Kojima T, Shworak N, Rosenberg RD. Molecular cloning and expression of two distinct cDNA-encoding heparan sulfate proteoglycan core proteins from a rat endothelial cell line. J Biol Chem 1992; 267: 4870-4877
14 Lindblom AI, Carlstedt I, Fransson LA. Identification of the core proteins in proteoglycans synthesized by vascular endothelial cells. Biochem J 1989; 261: 0145-0152
15 Mertens G, Cassiman JJ, van den Berghe H, Vermylen J, David G. Cell surface heparan sulfate proteoglycans from human vascular endothelial cells. Core protein characterization and antithrombin III binding properties J Biol Chem 1992; 267: 20435-20443
16 Shworak NW, Shirakawa M, Colliec-Jouault S, Liu J, Mulligen RC, Birinyi LK, Rosenberg RD. Pathway specific regulation of the synthesis of anticoagulantly active heparan sulfate. J Biol Chem 1994; 269: 24941-24949
17 Justus AC, Hoggatt AM, Faulk WP. Heparan-dependent endothelial antithrombin binding is increased by butyrate. Thromb Res 1995; 80: 0125-0133
18 Bauer PI, Machovich R, Aranyi R, Biiki G, Csonka E, Horvath I. Mechanism of thrombin binding to endothelial cells. Blood 1983; 61: 0368-0372
19 Shimada K, Ozawa T. Evidence that cell surface heparan sulfate is involved in the high affinity thrombin binding to cultured porcine aortic endothelial cells. J Clin Invest 1985; 75: 1308-1316
20 Hatton MWC, Moar SL. A role for pericellular proteoglycan in the binding of thrombin or antithrombin III by the blood vessel endothelium? The effects of proteoglycan-degrading enzymes and glycosaminoglycan-binding proteins on 125-I-thrombin binding by the rabbit aorta in vitro. Thromb Haemost 1985; 53: 0228-0234
21 Bartha K, Kovacs T, Lerant I, Papp B, Csonka E, Kolev K, Machovich R. Interaction of antithrombin III and thrombin-antithrombin III complex with cultured aortic endothelial cells. Thromb Res 1987; 47: 0541-0552
22 Stern D, Nawroth P, Marcum JA, Handley D, Kisiel W, Rosenberg RD, Stern K. Interaction of antithrombin III with bovine aortic segments: role of heparin in binding and enhanced anticoagulant activity. J Clin Invest 1985; 75: 0272-0279
23 Lollar P, Macintosh SC, Owen WG. Reaction of antithrombin III with thrombin bound to the vascular endothelium: analysis in a recirculating perfused rabbit heart preparation. J Biol Chem 1984; 259: 4335-4338
24 Nydah S, Frebelius S, Sweden borg. Thrombin inactivation and the effects of antithrombin and heparin in a recirculating Langendorff preparation. Thromb Res 1992; 65: 0365-0376
25 Delvos U, Meusel P, Preissner KT, Müller-Berghaus G. Formation of activated protein C and inactivation of cell-bound thrombin by antithrombin III at the surface of cultured vascular endothelial cells – a comparative study of two anticoagulant mechanism. Thromb Haemost 1987; 57: 0087-0095
26 Shimada K, Kobayashi M, Kimura S, Nishinaga M, Takeuchi K, Ozawa T. Anticoagulant heparin-like glycosaminoglycans on endothelial cell surface. Jpn Circ J 1991; 55: 1016-1021
27 Lowe-Krentz LJ, Joyce JG. Venous and aortic porcine endothelial cells cultured under standardized conditions synthesize heparan sulfate chains which differ in charge. Anal Biochem 1991; 193: 0155-0163
28 Justus AC, Roussev R, Norcross JL, Faulk WP. Antithrombin binding by human umbilical vein endothelial cells: effects of exogenous heparin. Thromb Res 1995; 79: 0175-0186
29 Marcum JA, McKenney JB, Rosenberg RD. The acceleration of thrombin-antithrombin complex formation in rat hindquarters via naturally occurring heparin-like molecules bound to the endothelium. J Clin Invest 1984; 74: 0341-0350
30 Xu Y, Slayter HS. Immunocytochemical localization of endogenous antithrombin III in the vasculature of rat tissues reveals location of anticoagulantly active heparan sulfate proteoglycans. J Histochem Cytochem 1994; 42: 1365-1372
31 Felsch JS, Owen WG. Endogenous antithrombin associated with microvasculature endothelium. Quantitative analysis in perfused rat hearts. Biochemistry 1994; 33: 0818-0822
32 Cooper ST, Neese LL, Di Cuccio MN, Liles DK, Hoffman M, Church FC. Vascular localization of the heparin-binding serpins antithrombin, heparin cofactor II, and protein C inhibitor. Thromb Haemost 1996; 2: 0185-0191
33 De Agostini Al, Watkins SC, Slayter HS, Youssoufian H, Rosenberg RD. Localization of anticoagulantly active heparan sulfate proteoglycans in vascular endothelium: antithrombin binding on cultured endothelial cells and perfused rat aorta. J Cell Biol 1990; 111: 1293-1304
34 Hatton MW, Moar SL, Richardson M. Evidence that rabbit¹²⁵I-antithrombin III binds to proteoheparin sulphate at the subendothelium of the rabbit aorta in vitro. Blood Vessels 1988; 25: 0012-0018
35 Hatton MWC, Moar SL, Richardson M. On the interaction of rabbit antithrombin III with the luminal surface of the normal and deendothelialized rabbit thoracic aorta in vitro. Blood 1986; 67: 0878-0886
36 Frebelius S, Hedin U, Swedenborg J. Thrombogenicity of the injured vessel wall – role of antithrombin and heparin. Thromb Haemost 1994; 71: 0147-0153
37 Busch C, Owen WG. Identification in vitro of an endothelial cell surface cofactor for antithrombin III. Parallel studies with isolated perfused rat hearts and microcarrier cultures of bovine endothelium. J Clin Invest 1982; 69: 0726-0734
38 Preissner KT, Müller-Berghaus G. Neutralisation and binding of heparin by S protein/vitronectin in the inhibition of factor Xa by antithrombin III. Involvment of an inducible heparin binding domain of S protein/vitronectin. J Biol Chem 1987; 262: 12247-12251
39 Williams EC, Huppert BJ, Asakura S. Neutralization of the anticoagulant effects of glycosaminoglycans by serum amyloid P component: comparison with other plasma and platelet proteins. J Lab Clin Med 1992; 120: 0159-0166
40 Preissner KT, De Boer H, Pannekoek H, De Groot PG. Thrombin regulation by physiological inhibitors: the role of vitronectin. Semin Thromb Haemost 1996; 22: 0165-0172
41 Blinder MA, Marasa JC, Reynolds CH, Deaven LL, Tollefsen DM. Heparin cofactor II: cDNA sequence, chromosome localization, restriction fragment length polymorphism, and expression in Escherichia coli. Biochemistry 1988; 27: 0752-0759
42 Struss DJ, Storck RE, Zimmermann P. The inhibition of thrombin and chymotrypsin by heparin cofactor II. Thromb Res 1992; 68: 0045-0056
43 Tollefsen DM. Insight into the mechanism of action of heparin cofactor II. Thromb Haemost 1995; 74: 1209-1214
44 Sié P, Dupouy D, Caranobe C, Petitou M, Boneu B. Antithrombotic properties of a dermatan sulfate hexasaccharide fractionated by affinity for heparin cofactor II. Blood 1993; 81: 1771-1777
45 Maimone MM, Tollefsen DM. Structure of a dermatan sulfate hexasaccharide that binds to heparin cofactor II with high-affinity. J Biol Chem 1990; 265: 18263-18271
46 Faber V, Quentin-Hoffmann E, Breuer B, Schittny J, Voler W, Kresse H. Colocalization of a large heterodimeric proteoglycan with basement membrane proteins in cultured cells. Eur J Cell Biol 1992; 59: 0037-0043
47 Schmidt G, Hausser H, Kresse H. Interaction of the small proteoglycan decorin with fibronectin. Involvement of the sequence NKISK of the core protein. Biochem J 1991; 280: 0411-0416
48 Vogel KG, Peterson DW. Extracellular, surface and intracellular proteoglycans produced by human embryo lung fibroblasts in culture (IMR-90). J Biol Chem 1981; 256: 13235-13240
49 järveläinen HT, Kinsella MG, Wight TN, Sandell LJ. Differential expression of small chondroitin/dermatan sulfate proteoglycans, PG-I/biglycan and PG-II/decorin, by vascular smooth muscle and endothelial cells in culture. J Biol Chem 1991; 266: 23274-23279
50 Bar-Shavit R, Eldor A, Vlodavsky I. Binding of thrombin to subendothelial extracellular matrix. Protection and expression of functional properties. J Clin Invest 1989; 84: 1096-1102
51 Schittny JC, Kresse H, Burri PH. Immunostaining of a heterodimeric dermatan sulphate proteoglycan is correlated with smooth muscles and some basement membranes. Histochem Cell Biol 1995; 103: 0271-0279
52 Kinsella MG, Wight TN. Formation of high molecular weight dermatan sulfate proteoglycan in bovine aortic endothelial cell cultures. Evidence for transglutaminase-catalyzed cross-linking to fibronectin. J Biol Chem 1990; 265: 17891-17898
53 Kresse H, Hausser H, Schonherr E, Bittner K. Biosynthesis and interactions of small chondroitin/dermatan sulfate proteoglycans. Eur J Clin Chem Clin Biochem 1994; 32: 0259-0266
54 Wight TN. Cell biology of arterial proteoglycans. Arteriosclerosis 1989; 9: 0001-0013
55 Cavari S, Vannucchi S. Glycosaminoglycans exposed on the endothelial cell surface. Binding of heparin-like molecules derived from serum. FEBS Lett 1993; 323: 0155-0161
56 McGuire EA, Tollefsen DM. Activation of heparin cofactor II by fibroblasts and vascular smooth muscle cells. J Biol Chem 1987; 262: 0169-0175
57 Kaji T, Itoh F, Hayakawa Y, Oguma Y, Sakuragawa N. Interaction of thrombin with heparin cofactor II and antithrombin III on prostacyclin production by cultured endothelial cells. Thromb Res 1989; 56: 0099-0108
58 Kaji T, Ejiri N, Hayakawa Y, Hayasi T, Sakuragawa N. Heparin cofactor II inhibits thrombin stimulated release of tissue plasminogen activator from cultured human endothelial cells in the presence of dermatan sulfate. Thromb Res 1990; 59: 0269-0277
59 Whinna HC, Choi HU, Rosenberg LC, Church FC. Interaction of heparin cofactor II with biglycan and decorin. J Biol Chem 1993; 268: 3920-3926
60 Shirk RA, Church FC, Wagner WD. Arterial smooth muscle cell heparan sulfate proteoglycans accelerate thrombin inhibition by heparin cofactor II. Arterioscler Thromb Vasc Biol 1996; 16: 1138-1146
61 Arisaka T, Mitsumata M, Kawasumi M, Tohjima T, Hirose S, Yoshida Y. Effects of shear stress on glycosaminoglycan synthesis in vascular endothelial cells. Ann NY Acad Sci 1995; 748: 0543-0553
62 Bendayan P, Boccalon H, Dupouy D. Boneu B+ Dermatan sulfate is a more potent inhibitor of clot-bound thrombin than unfractionated and low molecular weight heparins. Thromb Haemost 1994; 71: 0576-0582
63 Tollefsen DM, Pestka CA. Modulation of heparin cofactor II activity by histidine-rich glycoprotein and platelet factor 4. J Clin Invest 1985; 75: 0496-0502
64 Celia G, Boeri G, Saggiorato G, Paolini R, Luzzatto G, Terribile VI. Interaction between histidine-rich glycoprotein and platelet factor 4 with dermatan sulfate and low-molecular-weight dermatan sulfate. Angiology 1992; 43: 0059-0065
65 Zammit A, Dawes J. Fibrinogen inhibits the heparin cofactor II-mediated antithrombin activity of dermatan sulfate. Blood 1995; 85: 0720-0729
66 Rezaie AR, Esmon CT. Tryptophans 231 and 234 in protien C report the Ca(2+)-dependent conformational change required for activation by the thrombin-thrombomodulin complex. Biochemistry 1995; 34: 12221-12226
67 Harris KW, Esmon CT. Protein S is required for bovine platelets to support activated protein C binding and activity. J Biol Chem 1985; 260: 2007-2013
68 Fukudome K, Esmon CT. Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor. J Biol Chem 1994; 269: 26486-26492
69 Guinto ER, Esmon CT. Loss of prothrombin and of factor Xa-factor Va interactions upon inactivation of factor Va by activated protein C. J Biol Chem 1984; 259: 13986-13993
70 Shen L, Dahlback B. Factor V and protein S as synergistic cofactors to activated protein C in degradation of factor Villa. J Biol Chem 1994; 269: 18735-18741
71 Smirnov MD, Esmon CT. Phosphatidylethanolamine incorporation into , vesicles selectively enhances factor Va inactivation by activated protein C. J Biol Chem 1994; 269: 0816-0822
72 Stern DM, Nawroth PP, Harris K, Esmon CT. Cultured bovine aortic endothelial cells promote activated protein C-protein S-mediated inactivation of factor Va. J Biol Chem 1986; 261: 0713-0720
73 Suzuki K, Kusumoto H, Deyashiki Y, Nishioka J, Maruyama I, Zushi M, Kawahara S, Honda G, Yamamoto S, Hori guchi. Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation. EMBO J 1987; 6: 1891-1899
74 Tsiang M, Lentz S, Sadler JE. Functional domains of membrane bound thrombomodulin: EGF-like domains four to six and the serine/threonine rich domain are required for cofactor activity. J Biol Chem 1992; 267: 6164-6172
75 Parkinson JF, Nagashima M, Kuhn I, Jeonard J, Morser J. Structure-function studies of the epidermal growth factor domains of human thrombomodulin. Biochem Biophys Res Commun 1992; 185: 0567-0574
76 Gerlitz B, Hassell T, Vlahos CJ, Parkinson JF, Bang NU, Grinnell BW. Identification of the predominant glycosaminoglycan-attachment site in soluble recombinant human thrombomodulin: potential regulation of functionality by glycosyltransferase competition for serine 474. Biochem J 1993; 195: 0131-0137
77 Bourin MC, Lundgren-Akerlund E, Lindahl U. Isolation and characterization of the glycosaminoglycan component of rabbit thrombomodulin proteoglycan. J Biol Chem 1990; 265: 15424-15431
78 Maruyama I, Bell CE, Majerus PW. Thrombomodulin found on endothelium of arteries, veins, capillaries, and lymphatics and on syncy- tiothrophoblast of human placenta. J Cell Biol 1985; 101: 0363-0372
79 Suzuki K, Nishioka J, Hayashi T, Kosaka Y. Functionally active thrombomodulin is present in human platelets. J Biochem 1988; 104: 0628-0634
80 Ogura M, Ito T, Maruyama I, Takamatsu J, Yamamoto S, Ogawa K, Nagura H, Saito H. Localization and biosynthesis of functional thrombomodulin in human megakaryocytes and a human megakaryoblastic cell line (MEG-01). Thromb Haemost 1990; 64: 0297-0303
81 McCachren SS, Diggs J, Weinberg JB, Dittman WA. Thrombomodulin expression by human blood monocytes and by human synovial tissue lining macrophages. Blood 1991; 78: 3128-3136
82 Conway EM, Nowakowski B, Steiner-Mosonyi M. Human neutrophils synthesize thrombomodulin that does not promote thrombin-dependent protein C activation. Blood 1992; 80: 1254-1263
83 Soff GA, Jackman RW, Rosenberg RD. Expression of thrombomodulin by smooth muscle cells in culture: different effects of tumor necrosis factor and cyclic adenosine monophosphate on thrombomodulin expression by endothelial cells and smooth muscle cells in culture. Blood 1991; 77: 0515-0524
84 De Bault LE, Esmon NL, Olson JR, Esmon CT. Distribution of the thrombomodulin antigen in the rabbit vasculature. Lab Invest 1986; 54: 0179-0186
85 Wong V, Hofman F, Ishii H, Fisher M. Regional distribution of thrombomodulin in human brain. Brain Res 1991; 556: 0001-0009
86 Esmon CT, Esmon NL, Harris KW. Complex formation between thrombin and thrombomodulin inhibits both thrombin catalyzed fibrin formation and factor V activation. J Biol Chem 1983; 258: 12238-12244
87 Polgar J, Lerant I, Muszbek L, Machovich R. Thrombomodulin inhibits the activation of factor XIII by thrombin. Thromb Res 1986; 43: 0585-0595
88 Esmon NL, Carroll RC, Esmon CT. Thrombomodulin blocks the ability of thrombin to activate platelets. J Biol Chem 1983; 258: 12268-12273
89 Parkinson JF, Bang NU, Garcia JGN. Recombinant human thrombomodulin attenuates the inflammatory response of thrombin-stimulated human endothelial cells. Thromb Haemost 1991; 65: 0825-0832
90 Bourin MC, Lindahl U. Functional role of the polysaccharide component of rabbit thrombomodulin proteoglycan: effects on inactivation of thrombin by antithrombin, cleavage of fibrinogen by thrombin and thrombin- catalyzed activation of factor V. Biochem J 1990; 270: 0419-0425
91 Parkinson JF, Vlahos CJ, Yan SCB, Bang N. Recombinant human thrombomodulin: regulation of cofactor activity and anticoagulant function by chondroitin sulphate. Thromb Haemost 1991; 65: 0874-0883
92 Koyama T, Parkinson J, Sië P, Bang N, Müller-Berghaus G, Preissner KT. Different glycoforms of human thrombomodulin: their glycosaminogly-can-dependent modulatory effects on thrombin inactivation by heparin cofactor II and antithrombin III. Eur J Biochem 1991; 198: 0563-0571
93 Rezaie AR, Cooper ST, Church FC, Esmon CT. Protein C inhibitor is a potent inhibitor of the thrombin-thrombomodulin complex. J Biol Chem 1995; 270: 25336-25342
94 Ye J, Esmon NL, Esmon CT, Johnson A. The active site of thrombin is altered upon binding to thrombomodulin: two distinct structural changes are detected by fluorescence, but only one correlates with protein C activation. J Biol Chem 1991; 266: 23016-23022
95 Rezaie AR, Mather T, Sussman F, Esmon CT. Mutation of Glu 80 (to) Lys results in a protein C mutant that no longer requires Ca²⁺for rapid activation by the thrombin-thrombomodulin complex. J Biol Chem 1994; 269: 3151-3157
96 Parkinson J, Garcia J, Bang N. Decreased thrombin affinity of cell surface thrombomodulin following treatment of cultured endothelial cells with γ-D-xy1oside. Biochem Biophys Res Commun 1990; 169: 0177-0184
97 Thompson EA, Salem HH. Factors 1Xa, Xa, XIa and activated protein C do not have protein C activating ability in the presence of thrombomodulin. Thromb Haemost 1988; 59: 0339-0347
98 Freyssinet JM, Wiesel ML, Grunebaum L, Pereillo JM, Gauchy J, Schuhler S, Freund G, Cazenave JP. Activation of human protein C by blood coagulation factor Xa in the presence of anionic phospholipids: enhancement by sulphated polysaccharides. Biochem J 1989; 261: 0341-0348
99 Nishioka J, Ido M, Hayashi T, Suzuki K. The Gla(26) residue of protein C is required for the binding of protein C to thrombomodulin and endothelial cell protein C receptor, but not to protein S and factor Va. Thromb Haemost 1996; 75: 0275-0282
100 Steams-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex. Proc Natl Acad Sci USA 1996; 93: 10212-10216
101 Hirokawa K, Aoki N. Up-regulation of thrombomodulin in human umbilical vein endothelial cells in vitro. J Biochem Tokyo 1990; 108: 0839-0846
102 Horie S, Kizaki K, Ishii H, Kazama M. Retinoic acid stimulates expression of thrombomodulin, a cell surface anticoagulant glycoprotein, on human endothelial cells. Differences between up-regulation of thrombomodulin by retinoic acid and cyclic AMP. Biochm J 1992; 281: 0149-0154
103 Hirokawa K, Aoki N. Up-regulation of thrombomodulin by activation of histamine HI-receptors in human umbilical vein endothelial cells in vitro. Biochem J 1991; 276: 0739-0746
104 Kapiotis S, Besemer J, Bevec D, Valent P, Bettelheim P, Lechner K, Speiser W. Interleukin-4 counteracts pyrogen-induced down-regulation of thrombomodulin in cultured human vascular endothelial cells. Blood 1991; 78: 0410-0418
105 Hayashi T, Honda G, Suzuki K. An atherogenic stimulus homocysteine inhibits cofactor activity of thrombomodulin and enhances thrombomodulin expression in human umbilical vein endothelial cells. Blood 1992; 79: 2930-2939
106 Conway EM, Liu L, Nowakowski B, Steiner-Mosonyi M, Jackman RW. Heat shock of vascular endothelial cells induces an up-regulatory transcriptional response of the thrombomodulin gene that is delayed in onset and does not attenuate. J Biol Chem 1994; 269: 22804-22810
107 Takada Y, Shinkai F, Kondo S, Yamamoto S, Tsuboi H, Korenaga R, Ando J. Fluid shear stress increases the expression of thrombomodulin by cultured human endothelial cells. Biochem Biophys Res Commun 1994; 205: 1345-1353
108 Malek AM, Jackman R, Rosenberg RD, Izumo S. Endothelial expression of thrombomodulin is reversibly regulated by fluid shear stress. Circ Res 1994; 74: 0852-0861
109 Bartha K, Brisson C, Archipoff G, de-la Salle C, Lanza F, Cazenave JP, Beretz A. Thrombin regulates tissue factor and thrombomodulin mRNA levels and activities in human saphenous vein endothelial cells by distinct mechanisms. J Biol Chem 1993; 268: 0421-0428
110 Horie S, Ishii H, Hara H, Kazama M. Enhancement of thrombin-thrombomodulin-catalysed protein C activation by phosphatidylethanolamine containing unsaturated fatty acids: possible physiological significance of phosphatidylethanolamine in anticoagulant activity of thrombomodulin. Biochem J 1994; 301: 0683-0692
111 Slungaard A, Key NS. Platelet factor 4 stimulates thrombomodulin protein C-activating cofactor activity. A structure-function analysis. J Biol Chem 1994; 269: 25549-25555
112 Bourin MC, Boffa MC, Bjork I, Lindahl U. Functional domains of rabbit thrombomodulin. Proc Natl Acad Sci USA 1986; 83: 5924-5932
113 Archipoff G, Beretz A, Freyssinet J, Klein-Soyer C, Brisson C, Cazenave J. Heterogenous regulation of constitutive thrombomodulin of inducible tissue-factor activities on the surface of human saphenous-vein endothelial cells in culture following stimulation by interleukin-1, tumor necrosis factor, thrombin or phorbol ester. Biochem J 1991; 273: 0679-0686
114 Nawroth PP, Handley DA, Esmon CT, Stern DM. Interleukin-1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity. Proc Natl Acad Sci USA 1989; 83: 3460-3469
115 Moore KL, Andreoli SP, Esmon NL, Esmon CT, Bang NU. Endotoxin enhances tissue factor and suppresses thrombomodulin expression of human vascular endothelium in vitro. J Clin Invest 1987; 79: 0124-0132
116 Key NS, Vercellotti GM, Winkelmann JC, Moldow CF, Goodman JL, Esmon NL, Esmon CT, Jacob HS. Infection of vascular endothelial cells with herpes simplex virus enhances tissue factor activity and reduces thrombomodulin expression. Proc Natl Acad Sci USA 1990; 87: 7095-7103
117 Ogawa S, Gerlach H, Esposito C, Pasagian-Macaulay A, Brett J, Stern D. Hypoxia modulates the barrier and coagulant function of cultured bovine endothelium: increased monolayer permeability and induction of procoagulant properties. J Clin Invest 1990; 85: 1090-1099
118 Lentz SR, Sadler JE. Inhibtion of thrombomodulin surface expression and protein C activation by the thrombogenic agent homocysteine. J Clin Invest 1991; 88: 1906-1912
119 Glaser CB, Morser J, Clarke JH, Blasko E, McLean K, Kuhn I, Chang RJ, Lin JH, Vilander L, Andrews WH. Oxidation of a specific methionine in thrombomodulin by activated neutrophil products blocks cofactor activity. A potential rapid mechanism for modulation of coagulation. J Clin Invest 1992; 90: 2565-2574
120 Abe H, Okajima K, Okabe H, Takatsuki K, Binder BR. Granulocyte proteases and hydrogen peroxide synergistically inactivate thrombomodulin of endothelial cells in vitro. J Lab Clin Med 1994; 123: 0874-0883
121 Ohij T, Urano H, Shirahata A, Yamagishi M, Higashi K, Gotoh S, Karasaki Y. Transforming growth factor beta 1 and beta 2 induce down- modulation of thrombomodulin in human umbilical vein endothelial cells. Thromb Haemost 1995; 73: 0812-0819
122 Mukai HY, Ninomiya H, Ohtani K, Nagasawa T, Abe T. Major basic protein binding to thrombomodulin potentially contributes to the thrombosis in patients with eosinophilia. Br J Haematol 1995; 90: 0892-0899
123 Brighton TA, Chesterman CN. Antiphospholipid antibodies and thrombosis. Baillieres Clin Haematol 1994; 7: 0541-0553
124 Laszik Z, Carson CW, Nadasdy T, Johnson LD, Lerner MR, Brackett DJ, Esmon CT, Silva FG. Lack of suppressed renal thrombomodulin expression in a septic rat model with glomerular thrombotic microangiopathy. Lab Invest 1994; 70: 0862-0871
125 Drake TA, Cheng J, Chang A, Taylor FB. Expression of tissue factor, thrombomodulin, and E-selectin in baboons with lethal E. coli sepsis. Am J Pathol 1993; 142: 1458-1465
126 Schwalbe RA, Ryan J, Stern DM, Kisiel W, Dahlbäck B, Nelsestuen GL. Protein structural requirements and properties of membrane binding by 7-carboxyglutamic acid-containing plasma proteins and peptides. J Biol Chem 1989; 264: 20288-20295
127 Sugo T, Dahlbäck B, Holmgren A, Stenflo J. Calcium binding of bovine protein S. Effect of thrombin cleavage and removal of the 7-carboxyglutamic acid-containing region. J Biol Chem 1986; 261: 5116-5122
128 Dahlbäck B, Hildebrand B, Malm J. Characterization of functionally important domains in vitamin K-dependent protein S using monoclonal antibodies. J Biol Chem 1990; 265: 8127-8133
129 Dahlbäck B, Hildebrand B, Linse S. Novel type of very high-affinity calcium-binding sites in β-hydroxy-asparagine-containing epidermal growth factor-like domains in vitamin K-dependent protein S. J Biol Chem 1990; 265: 18481-18489
130 Nelson RM, Long GL. Binding of protein S to C4b-binding protein: mutagenesis of protein S. J Biol Chem 1992; 267: 8140-8148
131 Hardig Y, Dahlbäck B. The amino-terminal module of the C4b-binding protein beta-chain contains the protein S binding site. J Biol Chem 1996; 271: 20861-20867
132 Hillarp A. Molecular analysis of the beta-chain of human C4b-binding protein. Scand J Clin Lab Invest Suppl 1991; 204: 0057-0064
133 D’Angelo A, Vigano-D’Angelo S, Esmon CT, Comp PC. Acquired deficiences of protein S: protein S activity during oral anticoagulation, in liver disease and in disseminated intravascular coagulation. J Clin Invest 1988; 81: 1445-1453
134 Stem DM, Nawroth PP, Harris K, Esmon CT. Cultured bovine aortic endothelial cells promote activated protein C-protein S-mediated inactivation of factor Va. J Biol Chem 1986; 261: 0713-0719
135 Hackeng TM, Hessing M, van’t Veer C, Meijer-Huizinga F, Meijers JC, de-Groot PG, van Mourik JA, Bouma BN. Protein S binding to human endothelial cells is required for expression of cofactor activity for activated protein C. J Biol Chem 1993; 268: 3993-4000
136 Lu DS, Kalafatis M, Mann KG, Long GL. Comparison of activated protein C protein S-mediated inactivation of human factor VIII and factor V. Blood 1996; 87: 4708-4717
137 Shen L, Dahlbäck B. Factor V and protein S as synergistic cofactors to activated protein C in degradation of factor Villa. J Biol Chem 1994; 269: 18735-18743
138 Varadi K, Rosing J, Tans G, Pabinger I, Keil B, Schwarz HP. Factor V enhances the cofactor function of protein S in the APC-mediated inactivation of factor VIII: influence of the factor V-R506Q mutation. Thromb Haemost 1996; 76: 0208-0214
139 Nesheim ME, Canfield WM, Kisiel W, Mann KG. Studies of the capacity of factor Xa to protect factor Va from inactivation by activated protein C. J Biol Chem 1982; 257: 1443-1449
140 Jane SM, Hau L, Salem HH. Regulation of activated protein C by factor Xa. Blood Coag Fibrinolysis 1991; 2: 0723-0731
141 Solymoss S, Tucker MM, Tracy PB. Kinetics of inactivation of membrane-bound factor Va by activated protein C. Protein S modulates factor Xa protection. J Biol Chem 1988; 263: 14884-14889
142 Hackeng TM, van’t VeerC, Meijers JCM, Bouma BN. Human protein S inhibits prothrombinase complex activity on endothelial cells and platelets via direct interactions with factors Va and Xa. J Biol Chem 1994; 269: 21051-21058
143 Heeb MJ, Mesters RM, Tans G, Rosing J, Griffin JH. Binding of protein S to factor Va associated with inhibition of prothrombinase that is independent of activated protein C. J Biol Chem 1993; 268: 2827-2834
144 Heeb MJ, Rosing J, Bakker H, Fernandez JA, Tans G, Griffin JH. Protein S binds to and inhibits factor Xa. Proc Natl Acad Sci USA 1994; 91: 2728-2734
145 Koppelman SJ, Hackeng TM, Sixma JJ, Bouma BN. Inhibition of the intrinsic factor X activating complex by protein S: evidence for a specific binding of protein S to factor VIII. Blood 1995; 86: 1062-1071
146 Koppelman SJ, van’t Veer C, Sixma JJ, Bouma BN. Synergistic inhibition of the intrinsic factor X activation by protein S and C4b-binding protein. Blood 1995; 86: 2653-2662
147 van Wijnen M, Stam JG, van’t Veer C, Meijers JCM, Reitsma PH, Bertina RM, Bouma BN. The interaction of protein S with the phospholipid surface is essential for the activated protein C independent activity of protein S. Thromb Haemost 1996; 76: 0397-0403
148 van’t Veer C, Hackeng TM, Biesbroeck D, Sixma JJ, Bouma BN. Increased thrombin formation in protein S deficient plasma under flow conditions on endothelial cell matrix: an independent anticoagulant function of protein S in plasma. Blood 1995; 85: 0815-0823
149 Wun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze Jr GJ. Cloning and characterization of a cDNA for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988; 263: 6001-6008
150 Novotny WF, Girard TJ, Miletich JP, Broze Jr GJ. Purification and characterization of the lipoprotein-associated coagulation inhibitor from human plasma. J Biol Chem 1989; 264: 18832-18839
151 Novotny WF, Brown SG, Miletich JP, Rader DJ, Broze Jr GJ. Plasma antigen levels of the lipoprotein-associated coagulation inhibitor in patient samples. Blood 1991; 78: 0387-0393
152 Hoppensteadt DA, Walenga JM, Fasanella A, Jeske W, Fareed J. TFPI antigen levels in normal human volunteers after intravenous and subcutaneous administration of unfractionated heparin and a low molecular weight heparin. Thromb Res 1995; 77: 0175-0183
153 Broze Jr GJ, Warren LA, Novotny WF, Higuchi DA, Girard TJ, Miletich JP. The lipoprotein-associated coagulation inhibitor that inhibits factor VH-tissue factor complex also inhibits factor Xa: insight into its possible mechanism of action. Blood 1988; 71: 0335-0343
154 Girard TJ, Warren LA, Novotny WF, Likert KM, Brown SG, Miletich JP, Broze Jr GJ. Functional significance of the Kunitz-type inhibitory domains of lipoprotein-associated coagulation inhibitor. Nature 1989; 338: 0518-0523
155 Higuchi D, Wun TC, Likert KM, Broze Jr GJ. The effect of leukocyte elastase on tissue factor pathway inhibitor. Blood 1992; 79: 1712-1719
156 Nordfang O, Bjom SE, Valentin S, Nielsen LS, Wildgoose P, Beck TC, Hedner V. The C-terminus of tissue factor pathway inhibitor is essential to its anticoagulant activity. Biochemistry 1991; 30: 10371-10378
157 Wesselschmidt R, Likert K, Girard T, Wun TC, Broze Jr GJ. Tissue factor pathway inhibitor: the carboxy-terminus is required for optimal inhibition of factor Xa. Blood 1992; 79: 2004-2011
158 Hamamoto T, Yamamoto M, Nordfang O, Petersen JG, Foster DC, Kisiel W. Inhibitory properties of full-length and truncated recombinant tissue factor pathway inhibitor. Evidence that the third Kunitz-type domain of TFPI is not essential for the inhibition of factor Vlla- tissue factor complexes on cell surfaces. J Biol Chem 1993; 268: 8704-8711
159 Lindhout T, Franssen J, Willems G. Kinetics of the inhibition of tissue factor-factor Vlla by tissue factor pathway inhibitor. Thromb Haemost 1995; 74: 0910-0917
160 van’t Veer C, Hackeng TM, Delahaye C, Sixma JJ, Bouma BN. Activated factor X and thrombin formation triggered by tissue factor on endothelial cell matrix in a flow model: effect of the tissue factor pathway inhibitor. Blood 1994; 84: 1132-1139
161 Repke D, Gemmell CH, Guha A, Turitto VT, Broze Jr GJ, Nemerson Y. Hemophilia as a defect of the tissue factor pathway of blood coagulation: effect of factors VIII and IX on factor X activation in a continuous-flow reactor. Proc Natl Acad Sci USA 1990; 87: 7623-7629
162 Nordfang O, Valentin S, Beck TC, Hedner U. Inhibition of extrinsic pathway inhibitor shortens coagulation time of normal plasma and of hemophilia plasma. Thromb Haemost 1991; 66: 0464-0471
163 Welsch D, Novotny WF, Wun TC. Effect of lipoprotein-associated coagulation inhibitor (LACI) on thromboplastin-induced coagulation of normal and hemophiliac plasmas. Thromb Res 1991; 64: 0213-0221
164 Erhardtsen E, Ezban M, Madsen MT, Diness V, Glazer S, Hedner V, Nordfang Q. Blocking of tissue factor pathway inhibitor (TFPI) shortens the bleeding time in rabbits with antibody induced haemophilia A. Blood Coagul Fibrinolysis 1995; 6: 0388-0395
165 Rao LV, Nordfang O, Hoang AD, Pendurthi UR. Mechanism of antithrombin III inhibition of factor Vlla/tissue factor activity on cell surfaces. Comparison with tissue factor pathway inhibitor/factor Xa-induced inhibition of factor Vila/tissue factor activity. Blood 1995; 85: 0121-0129
166 Almus FE, Rao LV, Rapaport SI. Regulation of factor Vila/tissue factor functional activity in an umbilical vein model. Arterioscler Thromb 1993; 13: 0105-0111
167 Lindahl AK, Jacobsen PB, Sandset PM, Abildgaard U. Tissue factor path way inhibitor with high anticoagulant activity is increased in post-heparin plasma and in plasma from cancer patients. Blood Coagul Fibrinolysis 1991; 2: 0713-0722
168 Enjyoji K, Miyata T, Kamikubo Y, Kato H. Effect of heparin on the inhibition of factor Xa by tissue factor pathway inhibitor: a segment, Gly212-Phe243, of the third Kunitz domain is a heparin-binding site. Biochemistry 1995; 34: 5725-5732
169 Hamamoto T, Kisiel W. The effect of heparin on the regulation of factor Vlla-tissue factor activity by tissue factor pathway inhibitor. Blood Coagul Fibrinolysis 1996; 7: 0470-0476
170 Kaiser B, Hoppensteadt DA, Jeske W, Wun TC, Fareed J. Inhibitory effects of TFPI on thrombin and factor Xa generation in vitro-modulatory action of glycosaminoglycans. Thromb Res 1994; 75: 0609-0619
171 Hamamoto T, Kisiel W. Full-length tissue factor pathway inhibitor inhibits human activated protein C in the presence of heparin. Thromb Res 1995; 80: 0291-0302
172 Kojima T, Katsumi A, Yamazaki T, Muramatsu T, Nagasaka T, Ohsumi K, Saito H. Human ryudocan from endothelium-like cells binds basic fibroblast growth factor, midkine, and tissue factor pathway inhibitor. J Biol Chem 1996; 271: 5914-5920
173 Warshawsky I, Bu G, Mast A, Saffitz JE, Broze Jr GJ, Schwartz AL. The carboxy terminus of tissue factor pathway inhibitor is required for interacting with hepatoma cells in vitro and in vivo. J Clin Invest 1995; 95: 1773-1782
174 Valentin S, Reutelingsperger CPM, Nordfang O, Lindhout T. Inhibition of factor X activation at extracellular matrix of fibroblasts during flow conditions: a comparison between tissue factor pathway inhibitor and inactive factor Vlla. Thromb Haemost 1995; 74: 1478-1485
175 Valentin S, Schousboe I. Factor Xa enhances the binding of tissue factor pathway inhibitor to acidic phospholipids. Thromb Haemost 1996; 75: 0796-0800
176 Grabowski EF, Zuckerman DB, Nemerson Y. The functional expression of tissue factor by fibroblasts and endothelial cells under flow conditions. Blood 1993; 81: 3265-3274
177 Kazama Y, Komiyama Y, Kisiel W. Tissue factor pathway inhibitor and protease nexin-1 are major factor Xa binding proteins on the HepG2 cell surface. Blood 1993; 81: 0676-0683
178 Ho GY, Toomey JR, Broze GJ, Schwartz AL. Receptor-mediated endo-cytosis of coagulation factor Xa requires cell surface-bound tissue factor pathway inhibitor. J Biol Chem 1996; 271: 9497-9502
179 Lupu C, Lupu F, Dennehy U, Kakkar VV, Scully MF. Thrombin induces the redistribution and acute release of tissue factor pathway inhibitor from specific granules within human endothelial cell in culture. Arterioscler Thromb Vasc Biol 1995; 15: 2055-2061
180 Ameri A, Kuppuswamy MN, Basu S, Bajaj SP. Expression of tissue factor pathway inhibitor by cultured endothelial cells in response to inflammatory mediators. Blood 1992; 79: 3219-3226
181 Sprecher CA, Kisiel W, Mathewes S, Foster DC. Molecular cloning, expression, and partial characterization of a second human tissue-factorpathway inhibitor. Proc Natl Acad Sci USA 1994; 91: 3353-3360
182 Miyagi Y, Koshikawa N, Yasumitsu H, Miyagi E, Hirahara F, Aoki I, Misugi K, Umeda M, Miyazaki K. cDNA cloning and mRNA expression of a serine proteinase inhibitor secreted by cancer cells: identification as placental protein 5 and tissue factor pathway inhibitor-2. J Biochem Tokyo 1994; 116: 0939-0945
183 Kisiel W, Sprecher CA, Foster DC. Evidence that a second human tissue factor pathway inhibitor (TFPI-2) and human placental protein 5 are equivalent. Blood 1994; 84: 4384 (letter)
184 Petersen LC, Sprecher CA, Foster DC, Blumberg H, Hamamoto T, Kisiel W. Inhibitory properties of a novel human Kunitz-type protease inhibitor homologous to tissue factor pathway inhibitor. Biochemistry 1996; 35: 0266-0272
185 Römisch J, Pâques EP. Annexins: calcium-binding proteins of multi-functional importance?. Med Microbiol Immuno1 1991; 180: 0109-0119
186 Funakoshi T, Heimark RL, Hendrickson LE, McMullen BA, Fujikawa K. Human placental anticoagulant protein: isolation and characterization. Biochemistry 1987; 26: 5572-5579
187 Reutelingsperger CPM, Hornstra G, Hemker HC. Isolation and partial purification of a novel anticoagulant from arteries of human umbilical cord. Eur J Biochem 1985; 151: 0625-0632
188 Reutelingsperger CPM, Kop JM, Hornstra G, Hemker HC. Purification and characterization of a novel protein from bovine aorta that inhibits coagulation. Inhibition of the phospholipid dependent factor Xa-catalyzed prothrombin activation, through a high-affinity binding of the anticoagulant to the phospholipids. Eur J Biochem 1988; 173: 0171-0178
189 Sun J, Salem HH, Bird P. Nucleolus and cytoplasmatic localization of annexin V. FEBS Lett 1992; 314: 0425-0431
190 Römisch J, Schuler E, Bastian B, Burger T, Dunkel FG, Schwinn A, Hartmann AA, Pâques EP. Annexin I-VI quantitative determination in different human cell types and in plasma after myocardial infarction. Blood Coagulation and Fibrinolysis 1992; 3: 0011-0019
191 Flaherty MJ, West S, Heimark R, Fujikawa K, Tait FJ. Placental anticoagulant protein I. Measurement in extracellular fluids and cells of the haemostatic system. J Lab Clin Med 1990; 115: 0174-0183
192 Murphy CT, Peers SH, Forder RA, Flower RJ, Carey F, Westwick J. Evidence for the presence and location of annexins in human platelets. Biochem Biophys Res Commun 1992; 189: 1739-1745
193 Fujimagari M, Williamson PL, Schlegel RA. Ca²⁺-dependent membrane-binding proteins in normal erythrocytes and erythrocytes from patients with chronic myelogenous leukemia. Blood 1990; 75: 1337-1344
194 Tait JF, Gibson D, Fujikawa K. Phospholipid binding properties of human placental anticoagulant protein-I, a member of the lipocortin family. J Biol Chem 1989; 264: 7944-7951
195 Meers P, Mealy T. Calcium-dependent annexin V binding to phospholipids: stoichiometry, specificity, and role of negative charge. Biochemistry 1993; 32: 11711-11718
196 Concha NO, Head JF, Kaetzel MA, Dedman JR, Seaton BA. Annexin V forms calcium-dependent trimeric units on phospholipid vesicles. FEBS Lett 1992; 314: 0159-0164
197 Andree HAM, Stuart MCA, Hermens WTH, Reutelingsperger CPM, Hemker HC, Frederik PM, Willems GM. Clustering of lipid-bound annexin V may explain its anticoagulant effect. J Biol Chem 1992; 267: 17907-17913
198 Thiagarajam P, Tait JF. Binding of Annexin V/Placental anticoagulant protein I to platelets. J Biol Chem 1990; 265: 17420-17425
199 Tait JF, Gibson D. Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding. J Lab Clin Med 1994; 123: 0741-0750
200 Thiagarajam P, Tait JF. Collagen-induced exposure of anionic phospholipid in platelets and platelet-derived microparticles. J Biol Chem 1991; 266: 24302-24309
201 van Heerde WL, Poort S, van’t Veer C, Reutelingsperger CPM, de Groot PG. Binding of recombinant annexin V to endothelial cells: effect of annexin V binding on endothelial-cell-mediated thrombin formation. Biochem J 1994; 302: 0305-0311
202 Bombeli T, Karsan A, Tait JF, Harlan JM. Apoptotic endothelial cells become procoagulant. Blood. in press
203 Gramzinski RA, Broze Jr GJ, Carson SD. Human fibroblast tissue factor is inhibited by lipoprotein-associated coagulation inhibitor and placental anticoagulant protein but not by apolipoprotein A-II. Blood 1989; 73: 0983-0990
204 Ravanat C, Archipoff G, Beretz A, Freund G, Cazenave JP, Freyssinet JM. Use of annexin V to demonstrate the role of phosphatidylserine exposure in the maintenance of haemostatic balance by endothelial cells. Biochem J 1992; 282: 0007-0013
205 van Heerde WL, Sakariassen KS, Hemker HC, Sixma JJ, Reutelingsperger CP, de Groot PG. Annexin V inhibits the procoagulant activity of matrices of TNF-stimulated endothelium under blood flow conditions. Arterioscler Thromb 1994; 14: 0824-0832
206 Sun J, Bird P, Salem HH. Effects of annexin V on the activity of the anticoagulant proteins C and S. Thromb Res 1993; 69: 0279-0288
207 van Ryn-McKenna J, Merk H, Muller TH, Buchanan MR, Eisert WG. The effects of heparin and annexin V on fibrin accretion after injury in the jugular veins of rabbits. Thromb Haemost 1993; 69: 0227-0234
208 Römisch J, Seiffge D, Reiner G, Pâques EP, Heimburger N. In-vivo antithrombotic potency of placenta protein 4 (annexin V). Thromb Res 1991; 61: 0093-0102
209 van Nostrand WE, McKay LD, Baker JB, Cunningham DD. Functional and structural similarities between protease nexin-1 and Cl-inhibitor. J Biol Chem 1988; 263: 3979-3986
210 Sommer J, Gloor SM, Rovelli GF, Hofsteenge J, Nick H, Meier R, Monard D. cDNA sequence coding for a rat glia-derived nexin and its homology to members of the serpin superfamily. Biochemistry 1987; 26: 6407-6413
211 Eaton DL, Baker JB. Evidence that a variety of cultured cells secrete protease nexin and produce a distinct cytoplasmatic serine-protease-binding factor. J Cell Physiol 1983; 117: 0175-0183
212 Laug WE, Aebersold R, Jong A, Rideout W, Bergman BL, Baker JB. Isolation of multiple types of plasminogen activator inhibitor from vascular smooth muscle cells. Thromb Haemost 1989; 61: 0517-0524
213 Festoff BW, Rao JS, Hantai D. Plasminogen activators and inhibitors in the neuromuscular system: III. The serpin protease nexin I is synthesized by muscle and localized at neuromuscular synapses. J Cell Physiol 1991; 147: 0076-0084
214 Gronke RS, Curry TK, Baker JB. Formation of protease nexin-thrombin complexes on the platelet surface. J Cell Biochem 1986; 32: 0201-0209
215 Choi B, Suzuki M, Taiseung K, Wagner S, Cunningham DD. Protease nexin-1. Localization in the human brain suggests a protective role against extravasated serine proteases. Am J Path 1990; 137: 0741-0749
216 Knauer DJ, Cunningham DD. Protease nexins: cell-secreted proteins which regulate extracellular serine proteases. Trends Biochem Sci 1984; 9: 0231-0239
217 Hermans JM, Stone SR. Interaction of activated protein C with serpins. Biochem J 1993; 295: 0239-0245
218 Evans DL, McGrogan M, Scott RW, Carrell RW. Protease specificity and heparin binding and activation of recombinant protease nexin-1. J Biol Chem 1991; 266: 22307-22312
219 Wallace A, Rovelli G, Hofsteenge J, Stone SR. Effect of heparin on the glia-derived-nexin thrombin interaction. Biochem J 1989; 257: 0191-0199
220 Rovelli G, Stone SR, Guidolin A, Sommer J, Monard D. Characterization of the heparin-binding site of glia-derived nexin/protease nexin-1. Biochemistry 1992; 31: 3542-3549
221 Farrell DH, Cunnigham DD. Glycosaminoglycans on fibroblasts accelerate thrombin inhibition by protease nexin-I. Biochem J 1987; 245: 0543-0547
222 Hiramoto SA, Cunningham DD. Effects of fibroblasts and endothelial cells on inactivation of target proteases by protease nexin-1, heparin cofactor II and Cl-inhibitor. J Cell Biochem 1988; 36: 0199-0206
223 Farrell DH, Wagner SL, Yuan RH, Cunningham DD. Localization of protease nexin-1 on the fibroblast extracellular matrix. J Cell Physiol 1988; 134: 0179-0184
224 Vaughan PJ, Cunningham DD. Regulation of protease nexin-1 synthesis and secretion in cultured brain cells by injury-related factors. J Biol Chem 1993; 268: 3720-3727
225 Isaacs J, Savion N, Gospodarowicz D, Shuman MA. Effect of cell density of thrombin binding to a specific site on bovine vascular endothelial cells. J Cell Biol 1981; 90: 0670-0677
226 Leroy-Viard K, Jandrot-Perrus M, Tobelem G, Guillin MC. Covalent binding of human thrombin to a human endothelial cell-associated protein. Exp Cell Res 1989; 181: 0001-0009
227 Wagner SL, Lau AL, Cunningham DD. Binding of protease nexin-1 to the fibroblast surface alters its target proteinase specificity. J Biol Chem 1989; 264: 0611-0619
228 Donovan FM, Vaughan PJ, Cunningham DD. Regulation of protease nexin-1 target protease specificity by collagen type IV. J Biol Cem 1994; 269: 17199-17206
229 Knauer DJ, Thompson JA, Cunningham DD. Protease nexins: cell-secreted proteins that mediate the binding, internalization, and degradation of regulatory serine proteases. J Cell Physiol 1983; 117: 0383-0389
230 Rovelli G, Stone SR, Preissner KT, Monard D. Specific interaction of vitronectin with the cell-secreted protease inhibitor glia-derived nexin and its thrombin complex. Eur J Biochem 1990; 192: 0797-0806
231 Chang AC, Detwiler TC. Reactions of thrombin-serpin complexes with thrombospondin. Arch Biochem Biophys 1992; 299: 0100-0108
232 Ponte P, Gonzalez-Dewhitt P, Schilling J, Miller J, Hsu D, Greenburg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331: 0525-0531
233 Smith RP, Higuchi DA, Broze Jr GJ. Platelet coagulation factor Xla-inhibitor, a form of Alzheimer amyloid precursor protein. Science 1990; 248: 1126-1131
234 van Nostrand WE, Schmaier AH, Farrow JS, Cunningham DD. Protease nexin II (amyloid beta protein precursor): a platelet α-granule protein. Science 1990; 248: 0745-0751
235 van Nostrand WE, Schmaier AH, Farrow JS, Cines DB, Cunningham DD. Protease nexin II/amyloid beta-precursor in blood is a platelet-specific protein. Biochem Biophys Res Commun 1991; 175: 0015-0021
236 van Nostrand WE, Wagner SL, Suzuki M, Choi BH, Farrow JS, Geddes JW, Cotman CW, Cunningham DD. Protease nexin II a potent antichymo-trypsin, shows identity to amyloid beta-protein precursor. Nature 1989; 341: 0546-0550
237 van Nostrand WE, Wagner SL, Farrow JS, Cunningham DD. Immunopurification and protease inhibitory properties of protease nexin-2/amyloid beta-protein precursor. J Biol Chem 1990; 265: 9591-9599
238 Schmaier AH, Dahl LD, Rozemuller AJM, Roos RAC, Wagner SL, Chung R, van Nostrand WE. Protease nexin-2/amyloid beta-protein precursor. A tight binding inhibitor of coagulation factor IXa. J Clin Invest 1993; 92: 2540-2548
239 Schmaier AH, Dahl LD, Hasan AA, Cines DB, Bauer KA, van Nostrand WE. Factor IXa inhibition by protease nexin-2/amyloid beta-protein precursor on phospholipid vesicles and cell membranes. Biochemistry 1995; 34: 1171-1178
240 Johnson-Wood KL, Hendriksson T, Seubert F, Oltersdorf T, Lieberburg I, Schenk DB. Identification of secreted beta-amyloid precursor protein binding site on intact human fibroblasts. Biochem Biophys Res Commun 1994; 200: 1685-1690
241 van Nostrand WE, Schmaier AH, Wagner SL. Potential role of protease nexin-2/amyloid beta protein precursor as a cerebral anticoagulant. Ann N YAcad Sci 1992; 674: 0243-0249
242 Kaplan AP, Silverberg M, Ghebrehiwet B. The intrinsic coagulation/kinin pathway-the classical complement pathway and their interactions. Adv Exp Med Biol 1986; 198B: 0011-0014
243 Reddigari SR, Silverberg M, Kaplan AP. Assembly of the human plasma kinin-forming cascade along the surface of vascular endothelial cells. Int Arch Allergy Immunol 1995; 107: 0093-0095
244 Reddigari SR, Shibayama Y, Brunnee T, Kaplan AP. Human hageman factor (FXII) and high molecular weight kininogen compete for the same binding site on human umbilical vein endothelial cells. J Biol Chem 1993; 268: 11982-11987
245 Ratnoff OD, Everson B, Embury P, Ziats NP, Anderson JM, Emanuelson MM, Malemund CJ. Inhibition of the activation of Hageman factor (factor XII) by human vascular endothelial cell culture supernates. Proc Natl Acad Sci USA 1991; 88: 10740-10746
246 Kleniewski J, Donaldson VH. Endothelial cells produce a substance that inhibits contact activation of coagulation by blocking the activation of Hageman factor. Proc Natl Acad Sci USA 1993; 90: 0198-0202
247 Bombeli T, Muller M, Straub PW, Haeberli A. Cyclosporine induced detachment of vascular endothelial cells initiates the intrinsic coagulation system in plasma and in whole blood. J Lab Clin Med 1996; 6: 0621-0631
248 Rodgers GM, Greenberg CS, Shuman MA. Characterization of the effects of cultured vascular endothelial cells on the activation of blood coagulation. Blood 1983; 61: 1155-1162
249 Wuillemin WA, Minnema M, Meijers JC, Roem D, Eerenberg AJ, Nuijens JH, ten Cate H, Hack CE. Inactivation of factor XIa in human plasma assessed by measuring factor XIa-protease inhibitor complexes: major role for Cl-inhibitor. Blood 1995; 85: 1517-1526
250 Wuillemin WA, Eldering E, Citarella F, de Ruig CP, ten Cate H, Hack CE. Modulation of contact system proteases by glycosaminoglycans – selective enhancement of the inhibition of factor XIa. J Biol Chem 1996; 271: 12913-12918
251 Stem DM, Nawroth PP, Kisiel W, Vehar G, Esmon CT. The binding of factor IXa to cultured bovine aortic endothelial cells. Induction of a specific site in the presence of factors VIII and X. J Biol Chem 1985; 260: 6717-6722
252 Varadi K, Elodi S. Formation and functioning of the factor IXa-VIII complex on the surface of endothelial cells. Blood 1987; 69: 0442-0450
253 O’Brien DP, Johnson D, Byfield P, Tuddenham EGD. Inactivation of factor VIII by factor IXa. Biochemistry 1992; 31: 2805-2810
254 Lamphear BJ, Fay PJ. Proteolytic interactions of factor IXa with human factor VIII and factor Villa. Blood 1992; 80: 3120-3128
255 Vehar GA, Davie EW. Preparation and properties of bovine factor VIII (antihemophilic factor). Biochemistry 1980; 19: 0401-0409
256 Koppelman SJ, Koedam JA, Wijnen M, Stem DM, Nawroth PP, Sixma JJ, Bouma BN. von Willebrand factor as a regulator of intrinsic factor X activation. J Lab Clin Med 1994; 123: 0585-0592
257 Lajmanovich A, Hudry-Clergeon G, Freyssinet JM, Marguerie G. Human factor VIII procoagulant activity and phospholipid interaction. Biochim Biophys Acta 1981; 678: 0132-0139
258 Nesheim M, Pittman DD, Giles AR, Fass DN, Wang JH, Slonosky D, Kaufman RJ. The effect of plasma von Willebrand factor on the binding of human factor VIII to thrombin-activated human platelets. J Biol Chem 1991; 266: 17815-17822
259 Koedam JA, Hamer RJ, Beeser-Visser NH, Bouma BN, Sixma JJ. The effect of von Willebrand factor on the activation of factor VIII by factor Xa. Eur J Biochem 1990; 189: 0229-0234
260 Biedermann B, Rosenmund A, Müller M, Kohler HP, Haeberli A, Straub PW. Human endothelial cells suppress prothrombin activation in non-anticoagulated whole blood in vitro. J Lab Clin Med 1994; 124: 0339-0348
261 Kohler HP, Muller M, Bombeli T, Straub PW, Haeberli A. The suppression of the coagulation of non-anticoagulated whole blood in vitro by human umbilical vein endothelial cells cultivated on microcarriers is not dependent on protein C activation. Thromb Haemost 1995; 73: 0719-0726
262 Bombeli T, Müller M, Straub PW, Haeberli A. Human umbilical vein endothelial cells cultivated on microcarriers neutralize factor VIII: C in whole blood. Submitted for publication
263 De Stefano V, Finazzi G, Mannucci PM. Inherited thrombophilia: pathogenesis, clinical syndromes, and management. Blood 1996; 87: 3531-3544
264 Finazzi G, Caccia R, Barbui T. Different prevalence of thromboembolism in the subtypes of congenital antithrombin III deficiency: review of 404 cases. Thromb Haemost 1987; 58: 1094-1102
265 Chowdury V, Lane DA, Mille B, Auberger K, Gandenberger-Bachera S, Pabinger I, Olds RJ, Thein SL. Homozygous antithrombin deficiency: report of two new cases ("Leu to Phe) associated with arterial and venous thrombosis. Thromb Haemost 1994; 72: 0198-0205
266 Shibata S, Harpel PC, Gharavi A, Rand J, Fillit H. Autoantibodies to heparin from patients with antiphospholipid antibody syndrome inhibit formation of antithrombin III-thrombin complexes. Blood 1994; 83: 2532-2540
267 Shibata S, Sasaki T, Harpel PC, Fillit H. Autoantibodies to vascular heparan sulfate proteoglycan in systemic lupus erythematosus react with endothelial cells and inhibit the formation of thrombin-antithrombin III complexes. Clin Immunol Immunopathol 1994; 70: 0114-0123
268 Nishinaga M, Ozawa T, Shimada K. Homocysteine, a thrombogenic agent, suppresses anticoagulant heparan sulfate expression in cultured porcine aortic endothelial cells. J Clin Invest 1993; 92: 1381-1386
269 Deckert T, Horowitz IM, Kofoed-Enevoldsen A, Kjellen L, Deckert M, Lykkelund C, Burcharth F. Possilbe genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy. Diabetes 1991; 40: 0764-0770
270 Klein NJ, Shennan GI, Heyderman RS, Levin M. Alteration in glycosaminoglycan metabolism and surface charge on human umbilical vein endothelial cells induced by cytokines, endotoxin and neutrophils. J Cell Sci 1992; 102: 0821-0832
271 Key NS, Platt JL, Vercellotti GM. Vascular endothelial cell proteoglycan are susceptible to cleavage by neutrophils. Arterioscler Thromb 1992; 12: 0836-0842
272 Colburn P, Kobayashi E, Buonassisi V. Depleted level of heparan sulfate proteoglycan in the extracellular matrix of endothelial cell cultures exposed to endotoxin. J Cell Physiol 1994; 159: 0121-0130
273 Weisdorf DJ, Edson JR. Recurrent venous thrombosis associated with inherited deficiency of heparin cofactor II. Br J Haematol 1991; 77: 0125-0131
274 Sie P, Dupouy D, Pichon J, Boneu B. Constitutional heparin cofactor II deficiency associated with recurrent thrombosis. Lancet 1985; 2: 0414-0420
275 O’Driscoll A, Mackie IJ, Porter JB, Machin SJ. Low plasma heparin cofactor II levels in thalassaemia syndromes are corrected by chronic blood transfusion. Br J Haematol 1995; 90: 0065-0072
276 Porter JB, Young L, Mackie IJ, Marshall L, Machin SJ. Sickle cell disorders and chronic intravascular haemolysis are associated with low plasma heparin cofactor II. Br J Haematol 1993; 83: 0459-0462
277 Tollefsen DM, Pestka CA. Heparin cofactor II activity in patients with disseminated intravascular coagulation and hepatic failure. Blood 1985; 66: 0769-0776
278 Toulon P, Moulonguet-Doleris L, Costa JM, Aiach M. Heparin cofactor II deficiency in renal allograft recipients: no correlation with the development of thrombosis. Thromb Haemost 1991; 65: 0020-0027
279 Liu L, Dewar L, Song Y, Kulczycky M, Blajchman MA, Fenton JW, Andrew M, Delorme M, Ginsberg J, Preissner KT. Inhibition of thrombin by antithrombin III and heparin cofactor II in vivo. Thromb Haemost 1995; 73: 0405-0412
280 Kobayashi M, Shimada K, Ozawa T. Human recombinant interleukin-1 beta- and tumor necrosis factor alpha-mediated suppression of heparinlike compounds on cultured porcine aortic endothelial cells. J Cell Physiol 1990; 144: 0383-0390
281 Kaner RJ, Iozzo RV, Ziaie Z, Kefalides NA. Inhibition of proteoglycan synthesis in human endothelial cells after infection with herpes simplex virus type 1 in vitro. Am J Respir Cell Mol Biol 1990; 2: 0423-0429
282 Dahlback B. New molecular insights into the genetics of thrombophilia. Resistance to activated protein C caused by Arg⁵⁰⁶to G1n mutation in factor V as a pathogenic risk factor for venous thrombosis. Thromb Haemost 1995; 74: 0139-0148
283 Fijnvandraat K, Derkx B, Peters M, Bijlmer R, Sturk A, Prins MH, van Deventer SJ, ten Cate JW. Coagulation activation and tissue necrosis in meningococcal septic shock: severely reduced protein C levels predict a high mortality. Thromb Haemost 1995; 73: 0015-0023
284 Gruber A, Griffin JH, Harker LA, Hanson SR. Inhibition of platelet- dependent thrombus formation by human activated protein C in a primate model. Blood 1989; 73: 0639-0645
285 Hanson SR, Griffin JH, Harker LA, Kelly AB, Esmon CT, Gruber A. Antithrombotic effects of thrombin-induced activation of endogenous protein C in primates. J Clin Invest 1993; 92: 2003-2012
286 Muntean W, Finding K, Gamillscheg A, Zenz W. Multiple thrombosis and coumarin-induced skin necrosis in a young child with antiphospholipid antibodies. Thromb Haemorrh Disorders 1992; 5: 0043-0051
287 Okajima K, Imamura H, Koga S, Inoue M, Takatsuki K, Aoki N. Treatment of patients with disseminated intravascular coagulation by protein C. Am J Cardiol 1990; 33: 0277-0285
288 Gruber A, Hanson SR, Kelly AB, Yan BS, Bang NU, Griffin JH, Harker LA. Inhibition of thrombus formation by activated recombinant protein C in a primate model of arterial thrombosis. Circulation 1990; 82: 0578-0586
289 Kumada T, Dittman WA, Majerus PW. A role for thrombomodulin in the pathogenesis of thrombin-induced thromboembolism in mice. Blood 1988; 71: 0728-0733
290 Ohlin AK, Marlar RA. The first mutation identified in the thrombomodulin gene in a 45-year-old man presenting with thromboembolic disease. Blood 1995; 85: 0330-0338
291 Uchiba M, Okjima K, Murakami K, Nawa K, Okabe H, Takatsuki K. Recombinant human soluble thrombomodulin reduces endotoxin-induced pulmonary vascular injury via protein C activation in rats. Thromb Haemost 1995; 74: 1265-1270
292 Hasegawa N, Kandra TG, Husari AW, Veiss S, Hart WT, Hedgpeth J, Wydro R, Raffin TA. The effects of recombinant human thrombomodulin on endotoxin-induced multiple-system organ failure in rats. Am J Resp Crit Care Med 1996; 153: 1831-1837
293 D’Angelo A, Mazzola G, Bergmann F, Safa O, Della-Valle P, D’Angelo SV. Autoimmune protein S deficiency: a disorder predisposing to thrombosis. Haematologica 1995; 80: 0114-0119
294 Taylor Jr FB, Dahlbäck B, Chang AC, Lockhart MS, Hatanaka K, Peer G, Esmon CT. Role of free protein S and C4b binding protein in regulating the coagulant response to Escherichia coli. Blood 1995; 86: 2642-2652
295 Llobet D, Falkon L, Mateo J, Vallve C, Martinez E, Fontcuberta J, Borrell M. Low levels of tissue factor pathway inhibitor (TFPI) in two out of three members of a family with thrombophilia. Thromb Res 1995; 80: 0413-0422
296 Bajaj MS, Rana SV, Wysolmerski RB, Bajaj SP. Inhibitor of the factor Vlla-tissue factor complex is reduced in patients with disseminated intravascular coagulation but not in patients with severe hepatocellular disease. J Clin Invest 1987; 79: 1874-1883
297 Warr TA, Rao LVM, Rapaport SI. Human plasma extrinsic pathway inhibitor. I. Plasma levels in disseminated intravascular coagulation and hepatocellular disease. Blood 1989; 74: 0994-1002
298 Sandset PM, Roise O, Aasen AO, Abildgaard U. Extrinsic pathway inhibitor in postoperative/posttraumatic septicemia: increased levels in fatal cases. Haemostasis 1989; 19: 0189-0198
299 Shimura M, Wada H, Wakita Y, Nakase T, Hiyoyama K, Nagaya S, Mori Y, Shiku H. Plasma tissue factor and tissue factor pathway inhibitor levels in patients with disseminated intravascular coagulation. Am J Hematology 1996; 52: 0165-0170
300 Sandset PM, Wam-Cramer BJ, Rao LVM, Maki SL, Rapaport SI. Depletipn of extrinsic pathway inhibitor (EPI) sensitizes rabbits to disseminated intravascular coagulation induced with tissue factor: evidence supporting a physiologic role for EPI as a natural anticoagulant. Proc Natl Acad Sci USA 1991; 88: 0708-0717
301 Warn-Cramer BJ, Rapaport SI. Studies of factor Xa/phospholipid-induced intravascular coagulation in rabbits. Effects of immunodepletion of tissue factor pathway inhibitor. Arterioscler Thromb 1993; 13: 1551-1559
302 Creasey AA, Chang ACK, Feigen L, Wun TC, Taylor FB, Hinshaw LB. Tissue factor pathway inhibitor (TFPI) reduces mortality form E.coli septic shock. J Clin Invest 1993; 91: 2850-2858
303 Spokas EG, Wun TC. Venous thrombosis produced in the vena cava of rabbits by vascular damage and stasis. J Pharmacol Toxicol Methods 1992; 27: 0225-0231
304 Abendschein DR, Meng YY, Torr-Brown S, Sobel BE. Maintenance of coronary patency after fibrinolysis with tissue factor pathway inhibitor. Circulation 1995; 92: 0944-0949