Subscribe to RSS
DOI: 10.1055/s-0038-1656004
Ganglioside Content of Human Platelets – Differences in Resting and Activated Platelets
Publication History
Received 12 April 1996
Accepted after resubmission 04 November 1996
Publication Date:
11 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199703/lookinside/thumbnails/10-1055-s-0038-1656004-1.jpg)
Summary
Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBS A) content was 1.27 ± 0.04µεg/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 ± 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialyltransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.
-
References
- 1 Hakomori S, Kannagi R. Glycosphingolipids as tumor-associated and differentiation markers. J Natl Cancer Inst 1983; 71: 0231-0251
- 2 Perkins RM, Kellie S, Patel B, Critchley DR. Gangliosides as receptors for fibronectin. Exp Cell Res 1982; 141: 0231-0243
- 3 Cheresh DA, Pytela R, Pierschbacher MD, Klier FG, Ruoslahti E, Reisfeld RA. An Arg-Gly-Asp-directed receptor on the surface of human melanoma cells exists in a divalent cation-dependent functional complex with the disialoganglioside GD2. J Cell Biol 1987; 105: 1163-1173
- 4 Santoro SA. Inhibition of platelet adhesion to fibronectin, fibrinogen, and von Willebrand factor substrates by complex gangliosides. Blood 1989; 73: 0484-0489
- 5 Bremer EG, Hakomori SI, Bowen-Popo DF, Raines E, Ross R. Ganglio-sides-mediated modulation of cell growth, growth factor binding, and receptor phosphorylation. J Biol Chem 1984; 259: 6818-6825
- 6 Marcus AJ, Ullman HL, Safier LB. Studies on human platelet gangliosides. J Clin Invest 1972; 51: 2602-2612
- 7 Tamir H, Brunner W, Casper D, Rapport MM. Enhancement of gangliosides at the binding to serotonin binding protein. J Neurochem 1980; 34: 1719-1724
- 8 Gore PJ, Singh SP, Brooks DE. Composition of gangliosides from ovine testis and spermatozoa. Biochem Biophys Acta 1986; 876: 0036-0047
- 9 Mazurov AV, Prokazova NV, Mikhailenko IA, Mukjin DN, Repin VS, Berelson LD. Stimulation of platelet adhesion and activation by ganglioside GD3 adsorbed to plastic. Biochem Biophys Acta 1988; 968: 0167-0171
- 10 Chatterjee D, Anderson GM. The human platelet dense granule: serotonin uptake, tetrabenazine binding, phospholipid and ganglioside profiles. Arch Biochem Biophys 1993; 302: 0439-0446
- 11 Vasylevskaya VV, Zvezdina ND, Korotaeva AA, Prokazova NV. The influence of ganglioside on serotonin binding and uptake by human platelets. Platelets 1995; 6: 0037-0042
- 12 Wang CT, Schick PK. The effect of thrombin on the organization of human platelet membrane glycosphingolipids. J Biol Chem 1981; 256: 0752-0756
- 13 Tao RPV, Sweeley CC, Jamieson GA. Sphingolipid composition of human platelets. J Lipid Res 1973; 14: 0016-0025
- 14 Koerner TAW, Weinfeld HM, Yu RK, Bove JR. The effect of storage on the ganglioside content of human platelets. Transfusion 1986; 26: 0077-0081
- 15 Schick PK, He X. Composition and synthesis of glycolipids in megakaryocytes and platelets: differences in synthesis in megacaryocytes at different stages of maturation. J Lipid Res 1990; 31: 1645-1651
- 16 Rieber EP, Kniep B, Rank G. A membrane-protein-associated oligosaccharide defining functional T-cell subsets. In: Leukocyte typing IV. Knapp W, Dӧrken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, van dem Borne AEGKr. eds. Oxford; Oxford, UK: 1989. pp 0366-0368
- 17 Rieber EP. Cluster report: CDw60. In: Leukocyte typing IV. Knapp W, Dӧrken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, van dem Borne AEGKr. eds. Oxford; Oxford, UK: 1989. p 0361
- 18 Cheresh DA, Varki AP, Varki NM, Stallcup WB, Levine JM, Reisfeld RA. A monoclonal antibody recognizes an O-acetylated sialic acid in a human melanoma-associated ganglioside. J Biol Chem 1984; 259: 7453-7459
- 19 Cheresh DA, Reisfeld RA, Varki AP. O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic epitope. Science 1984; 225: 0844-0846
- 20 Thurin J, Herlyn M, Hindsgaul o, Stromberg N, Karlsson A, Elder D, Steplewski Z, Koprowski H. Proton NMR and fast atom bombardment mass spectrometry analysis of the melanoma-associated ganglioside 9-O-acetyl GD3. J Biol Chem 1985; 260: 14556-14563
- 21 Kniep B, Flegel WA, Northoff H, Rieber EP. CDw60 glycolipid antigens of human leukocytes: structural characterization and cellular distribution. Blood 1993; 82: 1776-1786
- 22 Aster RH, Jandl JH. Platelet sequestration in man. I. Methods. J Clin Invest 1964; 43: 0843-0855
- 23 Svennerholm L, Fredman P. A procedure for the quantitative isolation of brain gangliosides. Biochem Biophys Acta 1980; 617: 0097-0109
- 24 Jourdian GW, Dean L, Roseman S. A periodate-resorcinol method for the quantitative estimation of free sialic acid and their glycosides. J Biol Chem 1971; 246: 0430-0435
- 25 Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 0265-0271
- 26 Crook M. Sialic acid: its importance to platelet function in health and disease. Platelets 1991; 2: 0001-0010
- 27 Bosmann BH. Platelet adhesiveness and aggregation. Biochem Biophys Acta 1972; 279: 0456-0474
- 28 Bauvois B, Montreuil J, Verbert A. Characterization of a sialyl alpha 2-6 transferase from human platelets occurring in the sialylation of the N-glycosylproteins. Biochem Biophys Acta 1984; 788: 0234-0240