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Thromb Haemost 1997; 77(03): 548-554
DOI: 10.1055/s-0038-1656004
Platelets
Schattauer GmbH Stuttgart

Ganglioside Content of Human Platelets – Differences in Resting and Activated Platelets

Patrizia Ferroni
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
,
Luisa Lenti
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
,
Francesca Martini
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
,
Filippo Ciatti
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
,
Giuseppe M Pontieri
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
,
Pier Paolo Gazzaniga
The Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy
› Author Affiliations
Further Information

Publication History

Received 12 April 1996

Accepted after resubmission 04 November 1996

Publication Date:
11 July 2018 (online)

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Summary

Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBS A) content was 1.27 ± 0.04µεg/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 ± 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialyltransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.

 

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