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DOI: 10.1055/s-0038-1656009
Haemostatic Properties of Human Pulmonary and Cerebral Microvascular Endothelial Cells
Publication History
Received 30 August 1996
Accepted after revision 14 October 1996
Publication Date:
11 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199703/lookinside/thumbnails/10-1055-s-0038-1656009-1.jpg)
Summary
Little is known on the haemostatic profiles of human microvascular endothelial cells (MVEC) from different tissues. In addition it is not known whether MVEC from patients display the same haemostatic pattern as MVEC coming from healthy controls. To address these questions MVEC from human lung and brain were isolated and stimulated with tumour necrosis factor α (TNF) and E. coli lipopolysaccharide (LPS) for 24 h. The level and the kinetics of procoagulant activity (PCA) and thrombomodulin (TM) expression were found to be different depending on the tissue of origin and on the agonist used. In particular, the inducible PCA was higher in lung than in brain MVEC, an observation that may be related to the frequency of lung involvement in septic shock. Differences were also observed for tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) with MVEC supernatants or cell lysates. These variables were then measured in lung MVEC purified from patients with acute respiratory distress syndrome (ARDS) and compared to controls. Cells from ARDS patients constitutively expressed more PCA and PAI-1 than controls. The fibrinolytic potential, expressed as t-PA/PAI-1 ratio, was lower in ARDS than in lung MVEC. It is concluded that MVEC display different haemostatic features depending on the tissue they come from and that lung MVEC from ARDS patients present a procoagulant profile when compared with those from controls.
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References
- 1 Hewett PW, Murray JC. Human microvessels endothelial cells:isolation, culture and characterization. In Vitro Cell Dev Biol 1993; 29A: 0823-0830
- 2 Zetter BR. Endothelial heterogeneity:influence of vessel size, organ localization and species specificity on the properties of cultured endothelial cells. In: Endothelial Cells. Ryan US. ed. CRC Press; Boca Raton FL: 1988. 02. pp 0064-0079
- 3 Stolz DB, Jacobson BS. Macro-and microvascular endothelial cells in vitro:maintenance of biochemical heterogeneity despite loss of ultra-structural characteristics. In Vitro Cell Dev Biol 1991; 21 A: 0169-0182
- 4 Lodge PA, Haisch GE, Huber SA, Martin B, Craighead JC. Biological differences in endothelial cells depending upon organ differentiation. Transplant Proc 1991; 23: 0216-0218
- 5 Jackson CJ, Garbett PK, Nissen B, Schrieber L. Binding of human endothelium to Ulex europaeus-1 coated dyna-beds:application to the isolation of microvascular endothelium. J Cell Sci 1990; 96: 0257-0262
- 6 Carley WW, Niebalda MJ, Gerritsen ME. Isolation, cultivation, and partial characterization of microvascular endothelium derived from human lung. Am J Respir Cell Mol Biol 1992; 7: 0620-0630
- 7 Marks RM, Czerniecki M, Penny R. Human dermal microvascular endothelial cells:an improved method for tissue culture and a description of some singular properties in culture. In Vitro Cell Dev Biol 1985; 21: 0627-0635
- 8 Shatos MA, Orfeo T, Doherty JM, Penar PL, Collen D, Mann KG. Alpha-thrombin stimulates urokinase production and DNA synthesis in cultured human microvascular endothelial cells. Arterioscler Thromb Vase Biol 1995; 15: 0903-0911
- 9 Huynh HK, Dorovini-Zis K. Effects of interferon-gamma on primary cultures of humain brain microvessels endothelial cells. Am J Pathol 1993; 142: 1265-1278
- 10 Esmon CT, Schwartz HP. An update on clinical and basic aspects on the protein C anticoagulant pathway. Trends Cardiovascular Medicine 1995; 5: 0141-0148
- 11 Marcum JA, McKenney JB, Rosenberg RD. Acceleration of thrombin-anti-thrombin complex formation in rat hindquarters via heparin-like molecules bound to the endothelium. J Clin Invest 1984; 74: 0341-0350
- 12 Rosenberg RD, Rosenberg JS. Natural anticoagulant mechanisms. J Clin Invest 1984; 74: 0001-0006
- 13 Brox JH, sterud B, Bjorklid E, Fenton JW. 2d. Production and avaibility of thromboplastin in endothelial cells:the effect of thrombin, endotoxin and platelets. Br J Haematol 1984; 57: 0239-0245
- 14 Nawroth PP, Stern DM. Modulation of endothelial cell properties by tumor necrosis factor. J Exp Med 1986; 163: 0740-0745
- 15 More KL, Esmon CT, Esmon NL. Tumor necrosis factor leads to the internalization and degradation of thrombomodulin from the surface of bovine aortic endothelial cells in culture. Blood 1989; 73: 0159-0165
- 16 Colucci M, Paramo JA, Collen D. Generation in plasma of a fast-acting inhibitor in response to endotoxin stimulation. J Clin Invest 1985; 75: 0818-0824
- 17 Emeis JJ, Kooistra T. Interleukin-1 and lipopolysaccharide induce an inhibitor of tissue-type plasminogen activator in vivo and in cultured endothelial cells. J Exp Med 1986; 163: 1260-1266
- 18 Idell S, James KK, Levin EG, Schwartz BS, Manchada N, Maunder RJ, Martin TR, McLarty J, Fair DS. Local abnormalities in coagulation and fibrinolytic pathways predispose to alveolar fibrin deposition in the adult respiratory distress syndrome. J Clin Invest 1989; 84: 0695-0705
- 19 Bertozzi P, Astedt B, Zentius L, Lynch K, Lemaire F, Zapol W, Chapman HA. Depressed bronchoalveolar urokinase activity in patients with adult respiratory distress syndrome. N Engl J Med 1990; 322: 0890-0897
- 20 Hasegawa N, Husari AW, Hart WT. Role of coagulation system in ARDS. Chest 1994; 105: 0268-0277
- 21 de MoerloosePA, Hamilton JA, Sewell WA, Vadas MA, Mackay IR. Pertussigen in vivo enhances antigen-specific production in vitro of lymphokine that stimulates macrophage procoagulant activity and plasminogen activator. J Immunol 1986; 137: 3528-3533
- 22 de Moerloose P, de BenedettiE, Nicod L, Vifian C, Reber G. Procoagulant activity in bronchoalveolar lavage fluids:no relationship with tissue factor pathway inhibitor activity. Thromb Res 1992; 65: 0507-0518
- 23 Fuchs-Buder T, de MoerlooseP, Ricou B, Reber G, Vifian C, Nicod L, Romand J-A, Suter PM. Time course of procoagulant activity and D-dimer in bronchoalveolar fluid of patients at risk for or with acute respiratory distress syndrome. Am J Respir Crit Care Med 1996; 153: 0163-0167
- 24 Grau GE, Mili N, Lou JN, Morel DR, Ricou B, Lucas R, Suter PM. Phenotypic and functional analysis of pulmonary microvascular endothelial cells from patients with acute respiratory distress syndrome. Lab Invest 1996; 74: 0761-0770
- 25 Lou J, Chofflon M, Juillard C, Donati Y, Mili N, Siegrist CA, Grau GE. Brain microvascular endothelial cells and leukocytes derived from patients with multiple sclerosis exhibit increased adhesion capacity. Neuroreport. 1997. in press