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Thromb Haemost 1997; 77(04): 668-674
DOI: 10.1055/s-0038-1656031
Coagulation
Schattauer GmbH Stuttgart

Molecular Weight Measurements of Low Molecular Weight Heparins by Gel Permeation Chromatography

B Mulloy
1   The Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire
,
C Gee
1   The Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire
,
S F Wheeler
1   The Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire
,
R Wait
3   The Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, UK
,
E Gray
2   The Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire
,
T W Barrowcliffe
2   The Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire
› Author Affiliations
Further Information

Publication History

Received 28 May 1996

Accepted after revision 27 November 1996

Publication Date:
11 July 2018 (online)

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Summary

The molecular weight profiles of low molecular weight heparin samples have been measured by high-performance gel permeation chromatography using as calibrant the heparinase-degraded material (90/686) now established as the 1st International Reference Preparation (IRP) Low Molecular Weight Heparin for Molecular Weight Calibration. Use of the calibrant as a broad molecular weight standard is described and a calibration table provided based on data collected over several years in one laboratory.

In order to confirm the assignment of degree of polymerisation to resolved oligosaccharide peaks in the calibrant, molecular weights of oligosaccharides fractionated from the 1st IRP were independently determined by fast atom bombardment mass spectrometry (FAB MS).

The molecular weight distributions of commercial low molecular weight heparins have been characterized. Measurements of molecular weight parameters of heparin molecular weight standards from several sources provide comparisons between the molecular weight scales of this and other studies.

 

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