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Thromb Haemost 1997; 77(04): 772-782
DOI: 10.1055/s-0038-1656049
Vessel Wall
Schattauer GmbH Stuttgart

The Dietary Pigment Curcumin Reduces Endothelial Tissue Factor Gene Expression by Inhibiting Binding of AP-1 to the DNA and Activation of NF-κB

Angelika Bierhaus
1   The Department of Internal Medicine I, University of Heidelberg, Germany
,
Youming Zhang
1   The Department of Internal Medicine I, University of Heidelberg, Germany
,
Peter Quehenberger
2   The Allgemeines Krankenhaus Wien, Austria
,
Thomas Luther
3   The Institute for Pathology, Technical University Dresden, Germany
,
Michael Haase
3   The Institute for Pathology, Technical University Dresden, Germany
,
Martin Müller
3   The Institute for Pathology, Technical University Dresden, Germany
,
Nigel Mackman
4   The Department of Immunology, Scripps Institute, La Jolla, USA
,
Reinhard Ziegler
1   The Department of Internal Medicine I, University of Heidelberg, Germany
,
Peter P Nawroth
1   The Department of Internal Medicine I, University of Heidelberg, Germany
› Author Affiliations
Further Information

Publication History

Received 25 June 1996

Accepted after resubmission 19 December 1996

Publication Date:
11 July 2018 (online)

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Summary

The natural occurring pigment curcumin, a major component of the spice tumeric, has been described to have antioxidative, antitumorpro moting, anti-thrombotic and anti-inflammatory properties. It appears, that the pleiotropic effects of curcumin are at least partly due to inhibi tion of the transcription factors NF-κB and AP-1. This study investi gates the effect of curcumin on the TNFa induced expression of endo thelial Tissue Factor (TF), the central mediator of coagulation known to be controlled by AP-1 and NF-κ When bovine aortic endothelial cells (B AEC) were preincubated in the presence of curcumin, TNFα induced TF gene transcription and expression were reduced. Transient transfec tion studies with TF-promoter plasmids revealed that both, NF-κ and AP-1 dependent TF expression, were reduced by curcumin action. The observed inhibitions were due to distinct mechanisms. Curcumininhib ited TNFa induced kBa degradation and the nuclear import of NF-κB. In contrast, inhibition of AP-1 was due to a direct interaction of curcu min with AP-1-binding to its DNA binding motif. Thus, curcumin inhibits NF-κB and AP-1 by two different mechanisms and reduces expression of endothelial genes controlled by both transcription factors in vitro.

 

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