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Thromb Haemost 1997; 77(06): 1055-1061
DOI: 10.1055/s-0038-1656111
Clinical Studies
Schattauer GmbH Stuttgart

Molecular and Genetic Analysis of Two Patients with Bernard-Soulier Syndrome – Identification of New Mutations in Glycoprotein Ibα Gene

Taisuke Kanaji
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Takashi Okamura
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Mika Kuroiwa
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Masaaki Noda
1   Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
,
Kingo L Fujimura
1   Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
,
Atsushi Kuramoto
1   Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
,
Masayuki Sano
3   Department of Transfusion Medicine, Saga Medical School, Saga, Japan
,
Shuji Nakano
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Yoshiyuki Niho
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

Received 03 October 1995

Accepted after revision 06 March 1997

Publication Date:
12 July 2018 (online)

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Summary

We investigated two unrelated patients with Bernard-Soulier syndrome (BSS) by performing molecular and genetic analysis.

A flow cytometric and immunoblotting analysis showed GP Ibα to be absent from the platelet membrane of both patients. Other glycoproteins that formed GP Ib/IX/V complex were present on the platelets, but in decreased amounts. Therefore, GP Ibα gene from both cases was sequenced after PCR amplification and subcloning. We identified a homozygous mutation of a dinucleotide deletion within the TGTG repeat at cDNA number 972 to 975 in GP Ibα gene from Case 1. In Case 2, compound heterozygosity was demonstrated in GP Ibα gene; an insertion of a single base (T) at cDNA number 1,418 in one allele, and a deletion of a single base (A) within the 7-adenine repeat at cDNA number 1,438 to 1,444 in another allele. The three new mutations in both patients appeared to cause a frameshift, which created a new termination codon shortly thereafter, and thus lead to a GP Ibα deficiency on the platelet membrane. Truncated mutant proteins could be detected in the plasma and platelets of Case 2, but not of Case 1. According to these findings, it is thus supposed that the properties and conformation of additional COOH-terminal peptides, which were supposedly synthesized as results of the mutations, may have an important role on the processing of mutant GP Ibα in megakaryocytes and platelets.

 

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