Tissue Factor Expression during Monocyte-Macrophage Differentiation

Mark M E D van den Eijnden; Sari-lrina Steenhauer; Pieter H Reitsma; Rogier M Bertina

doi:10.1055/s-0038-1656125

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1997; 77(06): 1129-1136
DOI: 10.1055/s-0038-1656125

Coagulation

Schattauer GmbH Stuttgart

Tissue Factor Expression during Monocyte-Macrophage Differentiation

Authors

Mark M E D van den Eijnden

The Hemostasis and Thrombosis Research Center, Dept, of Hematology, University Hospital, Leiden, The Netherlands
Sari-lrina Steenhauer

The Hemostasis and Thrombosis Research Center, Dept, of Hematology, University Hospital, Leiden, The Netherlands
Pieter H Reitsma

The Hemostasis and Thrombosis Research Center, Dept, of Hematology, University Hospital, Leiden, The Netherlands
Rogier M Bertina

Abstract

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Summary

Tissue factor (TF), the high affinity receptor and cofactor of factor VII, is considered as the main procoagulant in stimulated monocytes and macrophages. We studied the effect of longterm culture (differentiation) on “spontaneous” and induced (LPS) expression of TF (mRNA, antigen, cell surface associated Vlla-cofactor activity) in isolated human monocytes.

TF was expressed transiently in monocytes cultured on Teflon membranes (suspension monocytes, Mo-S) and on plastic dishes (adherent monocytes, Mo-A), reaching maximal levels between days 3 and 5. Increased expression of TF was accompanied by increased stable expression of macrophage specific markers (CD71, the mannose receptor, the scavenger receptor).

Bacterial lipopolysaccharide (LPS) induced (additional) TF mRNA, antigen, and activity in both Mo-S and Mo-A. In Mo-S and Mo-A of days 3 to 5, the period in which there was “spontaneous” expression of TF, TF response to LPS was considerably lower.

It is concluded that during monocyte-macrophage transition, TF is “spontaneously” and transiently expressed and that with respect to TF induction the responsiveness of the cells to LPS is maintained.

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References

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