Observation of Factor XII and Factor XII Deficient Plasma at an Interface

Summary

Ellipsometry recordings and measurements, using wettable anodized tantalum slides (TaW), showed that an adsorbed film of factor XII deficient plasma could be partially removed by adding factor XII to the liquid medium.

When factor XII was first adsorbed onto the slide, addition of factor XII deficient plasma caused adsorption followed by partial desorption.

Adsorption from a mixture of factor XII and factor XII deficient plasma was followed by spontaneous partial desorption, thus showing a pattern similar to that of intact normal plasma.

The ability of a TaW surface to activate intact normal plasma was also demonstrated. The possibility is discussed that the sequence of factors XI and XII adsorption, interaction and desorption, as well as the possible resorption of their activation product, are a function of nature and amount of surface and duration of exposure.

• References

• 1 Boman H. G. Mutual displacement in protein chromatography. In: Symp. on Protein Structure, A. Neuberger Ed. 1957: 100-103 J. Wiley & Sons; New York:
• 2 DeVries S. I, Braat-Van Straaten M. A. J. Haemorrhagic diathesis as the result of severe deficiency of plasma thromboplastin antecedent (PTA, Factor XI). Thrombos. Diathes. haemorrh. (Stuttg.) 11: 167-186 1964;
• 3 Haanen C, Schoenmakers J. G. G. One-stage assay for Hageman factor (Factor XII). Further studies on the role of Hageman factor in blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 557-569 1963;
• 4 Hardisty R. M, Margolis J. The role of Hageman factor in the initiation of blood coagulation. Brit. J. Haemat. 5: 203-211 1959;
• 5 Iatridis S. G, Ferguson J. H. A two-stage assay method for Hageman factor activity. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 46-55 1962;
• 6 Margolis J. Initiation of blood coagulation by glass and related surfaces. J. Physiol. (Lond.) 137: 95-109 1957;
• 7 Margolis J. Some physical aspects of plasma/surface reactions. Proc. VIIIth Intern. Congress of Hemat., pp 1820-1825, 1960
• 8 Nossel H. L. The contact phase of blood coagulation. F. A. Davis, Co.; Philadelphia, Pa.: 1964
• 9 Ratnojf O. D, Davie E. W, Malleit D. L. Studies on the action of Hageman factor: evidence that activated Hageman factor in turn activates plasma, thromboplastin antecedent. J. clin. Inv. 40: 803-819 1961;
• 10 Soulier J.-P, Prou-Wartelle O. Nouvelles données sur les facteurs Hageman et P. T. A. et sur le “contact”. Rev. franc. Étud. clin. biol. 4: 932-938 1959;
• 11 Toald M, Wright I. S. Factor XI (P.T.A.) deficiency with no hemorrhagic symptoms. Thrombos. Diathes. haemorrh. (Stuttg.) 11: 187-194 1964;
• 12 Vroman L. Surface contact and thromboplastin formation. Thesis, Utrecht, Netherlands 1958
• 13 Vroman L. Blood coagulation studies with the recording ellipsometer. In: Ellipsometry in the measurement of surfaces and thin films. Nat. Bur. of Standards, Misc. pubi. 256:235-347, Symp. Proc. Washington 1963
• 14 Vroman L. Effects of hydrophobic surfaces upon blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 455-493 1964;
• 15 Vroman L. Behavior of coagulation factors at interfaces. Proc. Internat. Conf. Biophysical Mechanisms in Vase. Homeostasis and Intravas. Thromb. State U. N. Y. Downstate Med. Center 1964. Appleton-Century-Crofts, New York. (In press.)
• 16 Vroman L, Lukosevicius A. Ellipsometer recordings of changes in optical thickness of adsorbed films associated with surface activation of blood clotting. Nature 204: 701-703 1964;
• 17 Waaler B. A. Contact activation in the intrinsic blood clotting system. Scand. J. clin. Lab. Invest. 11 (Suppl. 37) 1-130 1959;