Subscribe to RSS
DOI: 10.1055/s-0038-1656298
The in Vitro Anticoagulant Activity of Human Plasma Lipids[*]
Publication History
Publication Date:
27 June 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/196501/lookinside/thumbnails/10-1055-s-0038-1656298-1.jpg)
Summary
The present report describes the in vitro inhibition of prothrombin consumption by lipid extracted from normal human platelet-poor plasma. Pro-coagulant lipids and the test plasma lipids were dissolved in the same solvent and evaporated to dryness. The dried lipid mixture was emulsified in platelet-poor plasma, and the plasma clotted by recalcification. Inhibition of prothrombin consumption by plasma lipid was observed when the ratio of plasma lipid to procoagulant lipid equaled or exceeded 5:1. The inhibitory effect of plasma lipid on prothrombin consumption was not observed when procoagulant activity was provided by intact platelets or platelet granules.
Human plasma lipids have been fractionated on silicic acid columns to permit identification of the plasma lipid component responsible for the inhibition of prothrombin consumption. The inhibitory activity is present in a lipid fraction which is 95% lecithin. Other plasma lipid components exhibit little or no inhibitory activity.
* These studies were supported in part by Grant H-04167 (hem) from the National Heart Institute, Bethesda, Maryland, and in part by contract DA-49-007-MD-632, Office of the Surgeon General, Department of the Army.
** Trainee under Training Grant 5-T1-am 5113-07, NI AMD, National Institutes of Health, Bethesda, Maryland.
-
References
- 1 Zak E. Studien zur Blutgerinnungslehre. Arch. exp. Path. Pharmakol. 74: 1 1913;
- 2 Chargajf E. Studies on chemistry of blood coagulation; lipid inhibitor of blood clotting occurring in mammalian tissue. J. Biol. Chem. 121: 175 1937;
- 3 de Sütö-Nagy G. J. The chemical nature of an anticoagulant from tissues. J. Biol. Chem. 250: 433 1944;
- 4 Hecht E, Shapiro D. Sphingosine as an inhibitor of blood clotting. Science 125: 1041 1957;
- 5 Tocantins L. M, Carroll R. T. The separation and assay of a lipid antithromboplastin from human brain, blood and plasma fractions. Trans, of the Second Conf. on Blood Clotting and Allied Problems, p. 11. Josiah Macy, Jr. Foundation, New York, N. Y. 1949
- 6 Turner D. L, Silver M. J, Tocantins L. M. Phospholipid antithromboplastin. II. Preparation and properties. Arch. Biochem. 77: 249 1958;
- 7 Barkhan P, Silver M. J, de Costa P. B, Tocantins L. M. Phosphatidyl serine and blood coagulation. Nature 182: 1031 1958;
- 7a Silver M. J, Turner D. L, Rodalewicz I, Giordano N, Holburn R, Herb S. F, Luddy F. E. Evaluation of activity of phospholipids in blood coagulation in vitro. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 164 1963;
- 7b Holburn R. R, Silver M. J. Further studies on the inhibitory activity of phosphatidyl serine in blood coagulation in vitro. Fed. Proc. 23: 300 1964;
- 7c Mustard J. F, Medway W, Downie H. G, Rowsell H. C. Effect of intravenous phospholipid containing phosphatidyl serine on blood clotting with particular reference to the Russell’s viper venomtime. Nature 196: 1063 1962;
- 8 Troup S. B, Reed C. F, Marinetti G. V, Swisher S. N. Thromboplastic factors in platelets and red blood cells: Observations on their chemical nature and function in in vitro coagulation. J. clin. Invest. 39: 342 1960;
- 9 Phillips G. B. The isolation and quantitation of the principal phospholipid components of human serum using chromatography on silicic acid. Biochim. biophys. acta (Amst.) 49: 286 1961;
- 10 Nye W. H, WaterHouse C, Marinetti G. V. The phosphatides of human plasma. I. Normal values determined by paper and column chromatography. J. clin. Invest. 40: 1194 1961;
- 11 Overman R. S. The chemical purification and mode of action of thromboplastic inhibitor. Trans, of the Second Conf. on Blood Clotting and Allied Problems. New York, N. Y., Josiah Macy, Jr. Foundation, p. 29 1949
- 12 Rapport M. M. Activation of phospholipid thromboplastin by lecithin. Nature 178: 591 1956;
- 13 Schulz H, Hiepler E. Über die Lokalisierung von gerinnungsphysiologischen Aktivitäten in submikroskopischen Strukturen der Thrombozyten. Klin. Wschr. 37: 273 1959;
- 14 Reed G. F, Eden E, Marinetti G. V, Swisher S. N. Studies of erythrocyte phospholipids. J. Lab. clin. Med. 56: 281 1960;
- 15 Marinetti G. V, Erbland J, Kochen J. Quantitative chromatography of phosphatides. Fed. Proc. 16: 837 1957;
- 16 Hanahan D. J. Lipid Chemistry. 28 John Wiley and Sons; New York, N. Y.: 1960
- 17 Alexander B. One stage method for specific prothrombin determination (in plasma or serum). In: Coagulation of Blood. Methods of Study. Ed. Tocantins L. M. 94 Grune and Stratton; New York, N. Y.: 1955