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Thromb Haemost 1992; 68(02): 119-124
DOI: 10.1055/s-0038-1656335
Original Article
Schattauer GmbH Stuttgart

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

F G Falkner
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
P L Turecek
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
R T A MacGillivray
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
W Bodemer
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
F Scheiflinger
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
S Kandels
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
A Mitterer
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
O Kistner
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
N Barrett
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
J Eibl
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
,
F Dorner
The Immuno AG, Biomedical Research Center, Orth/Donau, Austria, and the University of British Columbia, Department of Biochemistry, Vancouver, B. C., Canada
› Author Affiliations
Further Information

Publication History

Received 24 August 1990

Accepted after revision 02 March 1992

Publication Date:
03 July 2018 (online)

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Summary

We have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

 

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