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Thromb Haemost 1992; 68(03): 341-345
DOI: 10.1055/s-0038-1656377
Original Article
Schattauer GmbH Stuttgart

Mass Contents of Inositol 1,4,5-Trisphosphate and 1,2-Diacylglycerol in Human Platelets Stimulated with a Thromboxane Analogue and Thrombin

A Suganuma
1   The Department of Drug Safety Research, Eisai Co., Ltd., Gifu, Japan
,
S Nakashima
The Department of Biochemistry, Gifu University School of Medicine, Gifu, Japan
,
Y Okano
The Department of Biochemistry, Gifu University School of Medicine, Gifu, Japan
,
Y Nozawa
The Department of Biochemistry, Gifu University School of Medicine, Gifu, Japan
› Author Affiliations
Further Information

Publication History

Received 13 December 1991

Accepted after revision 09 April 1992

Publication Date:
04 July 2018 (online)

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Summary

Mass contents of inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol (DG) were measured in U46619-stimulated human platelets. 1 µM of U46619 induced maximum responses in aggregation, 5-hydroxytryptamine (5HT) secretion and increase in intracellular free Ca2+ concentration ([Ca2+]i). Aggregation was almost comparable to that induced by maximal dose (1 U/ml) of thrombin, while 5HT release was almost half. The initial [Ca2+]i peak in response to U46619 was about half of thrombin stimulation. Production of IP3 and DG was, however, less than one tenth of that seen in thrombin stimulation. The profile (time course and concentration-dependency) of IP3 formation did not correlate with that of [Ca2+]i, suggesting that U46619 stimulates IPs-dependent and -independent Ca2+ mobilization. DG production was small but sustained for more than 5 min. These findings support the recent hypothesis that aggregation is regulated by a delayed accumulation of DG. The low level of 5HT secretion could be explained by the low production of second messengers, IP3 and DG.

 

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