The Isoelectric Point of Thrombin and its Behaviour Compared to Prothrombin at Some Solid Surfaces

Walter Berg; Birgitta Hillvärn; Hans Arwin; Manne Stenberg; Ingemar Lundström

doi:10.1055/s-0038-1656988

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 42(03): 972-982
DOI: 10.1055/s-0038-1656988

Original Article

Schattauer GmbH Stuttgart

The Isoelectric Point of Thrombin and its Behaviour Compared to Prothrombin at Some Solid Surfaces

Walter Berg

^*The Clinical Investigation Service, Vasa Hospital Gothenburg, Sweden

,

Birgitta Hillvärn

^*The Clinical Investigation Service, Vasa Hospital Gothenburg, Sweden

,

Hans Arwin

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden

,

Manne Stenberg

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden

,

Ingemar Lundström

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden

› Author Affiliations

Abstract

PDF Download

Summary

We have shown that the isoelectric point of thrombin is high and that thrombin is a cation at the pH of blood. On the other hand, prothrombin has a low isoelectric point, being more anionic at the pH of blood. It was also found that thrombin adsorbs readily to surfaces, especially negatively charged surfaces, like behenic acid surfaces at pH 8.2. Furthermore, thrombin adsorbed onto behenic acid was active in the sense that it coagulated fibrinogen.

The significance of the electric charge of the thrombin molecule in the mechanism of atherosclerosis is discussed.

PDF (389 kb)

References

References
1 Berg W, Korsan-Bengtsen K, Ygge J. 1966; Human and plasminogen-free thrombin, purified by means of gel filtration and ion exchange chromatography. Thrombosis et Diathesis Haemorrhagica 75: 501
2 Berg W, Korsan-Bengtsen K. 1963; Separation of human fibrinogen and plasminogen by means of gel filtration. Thrombosis et Diathesis Haemorrhagica 9: 151
3 Constantinroes P, Vancouver BC. 1969. Ultrastructural studies on the role of endothelial changes in the pathogenesis of atherosclerosis and thrombosis. In Schettler G. (ed.) Platelets and the Vessel Wall-fibrin deposition. Symposium of the European Atherosclerosis Group. Georg Thieme Verlag; Stuttgart: p 94
4 Levine WG, Neuhaus OW. 1959; An electrophoretic study of purified thrombin. Proceedings of the Society for Experimental Biology and Medicine, (N. Y.) 101: 64
5 Mustard JF, Kinlough-Rathbone RL, Packham MA. 1974; Recent status of research in the pathogenesis of thrombosis. Thrombosis et Diathesis Haemorrhagica 59: 157
6 Mustard JF, Packham MA. 1975; The role of blood and platelets in atherosclerosis and the complications of atherosclerosis. Thrombosis et Diathesis Haemorrhagica 33: 444
7 Poste G, Moss C. 1972; The study of surface reactions in biological systems by ellipsometry. Progress in Surface Science Vol. 2, Part 3 p. 139-p. 232
8 Ross R, Glomset JA. 1976; The pathogenesis of atherosclerosis. New England Journal of Medicine 295 369: 420
9 Rothen A. 1973; Immunologic and enzymatic reactions carried out at a solid-liquid interface. Physiological Chemistry and Physics 5 p. 243
10 Seegers WH, Harmison CR, Ivanovic N, Heene D. 1966; Free boundary electrophoresis of purified bovine prothrombin and thrombin. Thrombosis et Diathesis Haemorrhagica 15: 343
11 Seegers WH, McCoy L, Kipper RK, Muraus G. 1968; Preparation and properties of thrombin. Archives of Biochemistry and Biophysics 128: 194
12 Small DM. 1977; Cellular mechanisms for lipid depositions in atherosclerosis. New England Journal of Medicine 297 872: 924
13 Vroman L, Lukosevicius A. 1964; Ellipsometer recordings on changes in optical thickness of adsorbed films associated with surface activation of blood clotting. Nature 14: 701
14 Vroman L. 1967. Surface activity in blood coagulation. In Seegers WH. (ed.) Blood Clotting Enzymology. Academic Press; New York: p 279