The Isoelectric Point of Thrombin and its Behaviour Compared to Prothrombin at Some Solid Surfaces

Walter Berg; Birgitta Hillvärn; Hans Arwin; Manne Stenberg; Ingemar Lundström

doi:10.1055/s-0038-1656988

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 42(03): 972-982
DOI: 10.1055/s-0038-1656988

Original Article

Schattauer GmbH Stuttgart

The Isoelectric Point of Thrombin and its Behaviour Compared to Prothrombin at Some Solid Surfaces

Authors

Walter Berg

^*The Clinical Investigation Service, Vasa Hospital Gothenburg, Sweden
Birgitta Hillvärn

^*The Clinical Investigation Service, Vasa Hospital Gothenburg, Sweden
Hans Arwin

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden
Manne Stenberg

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden
Ingemar Lundström

^**The Research Laboratory of Electronics, Chalmers University of Technology, Gothenburg, Sweden

Abstract

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Summary

We have shown that the isoelectric point of thrombin is high and that thrombin is a cation at the pH of blood. On the other hand, prothrombin has a low isoelectric point, being more anionic at the pH of blood. It was also found that thrombin adsorbs readily to surfaces, especially negatively charged surfaces, like behenic acid surfaces at pH 8.2. Furthermore, thrombin adsorbed onto behenic acid was active in the sense that it coagulated fibrinogen.

The significance of the electric charge of the thrombin molecule in the mechanism of atherosclerosis is discussed.

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References

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