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Thromb Haemost 1979; 42(04): 1153-1158
DOI: 10.1055/s-0038-1657010
Original Article
Schattauer GmbH Stuttgart

Fibrin Suspension as a Substrate for Plasmin: Determination and Kinetics

Shozo Kanai
The Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, MegurokUy Tokyo 152, Japan
,
Hiroshi Okamoto
The Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, MegurokUy Tokyo 152, Japan
,
Yutaka Tamaura
The Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, MegurokUy Tokyo 152, Japan
,
Shojiro Yamazaki
The Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, MegurokUy Tokyo 152, Japan
,
Yuji Inada
The Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, MegurokUy Tokyo 152, Japan
› Author Affiliations
Further Information

Publication History

Received 18 August 1978

Accepted 04 April 1979

Publication Date:
23 August 2018 (online)

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Summary

Fibrin polymers formed from fibrinogen with thrombin in the presence of EDTA were suspended in a medium containing glucose, arabic gum and imidazole-HCl buffer and were sonicated at 20 kHz for 20 min to make a suspension containing fibrin particles of small size. The fibrin suspension was used as a substrate of plasmin for determining the enzymic activity of plasmin and plasminogen activated with urokinase. The kinetic study on the reaction of the fibrin particles with plasmin in the presence and the absence of fibrinogen revealed that Km value of fibrin for plasmin is 4.2 × 10-7 M and the Ki value of fibrinogen is 1.2 × 10-5 M.

 

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