Study of Platelet Agglutination Induced by the Antibiotics of the Vancomycin Group: Ristocetin, Ristomycin, Actinoidin and Vancomycin

Zoltan Boda; Nils Olav Solum; Ferenc Sztaricskai; Kalman Rak

doi:10.1055/s-0038-1657012

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 42(04): 1164-1180
DOI: 10.1055/s-0038-1657012

Original Article

Schattauer GmbH Stuttgart

Study of Platelet Agglutination Induced by the Antibiotics of the Vancomycin Group: Ristocetin, Ristomycin, Actinoidin and Vancomycin

Authors

Zoltan Boda

The IInd Department of Medicine, University Medical School, 4012 Debrecen, Hungary, the Institute for Thrombosis Research, University of Oslo, Norway and the Research Group of Antibiotics, Hungarian Academy of Sciences, Lajos Kossuth University, 4012 Debrecen, Hungary
Nils Olav Solum

The IInd Department of Medicine, University Medical School, 4012 Debrecen, Hungary, the Institute for Thrombosis Research, University of Oslo, Norway and the Research Group of Antibiotics, Hungarian Academy of Sciences, Lajos Kossuth University, 4012 Debrecen, Hungary
Ferenc Sztaricskai

The IInd Department of Medicine, University Medical School, 4012 Debrecen, Hungary, the Institute for Thrombosis Research, University of Oslo, Norway and the Research Group of Antibiotics, Hungarian Academy of Sciences, Lajos Kossuth University, 4012 Debrecen, Hungary
Kalman Rak

The IInd Department of Medicine, University Medical School, 4012 Debrecen, Hungary, the Institute for Thrombosis Research, University of Oslo, Norway and the Research Group of Antibiotics, Hungarian Academy of Sciences, Lajos Kossuth University, 4012 Debrecen, Hungary

Abstract

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Summary

Four antibiotics which belong to the vancomycin group have been examined in respect of their effects on the factor VIII dependent platelet agglutination. A striking similarity between ristocetin and ristomycin was observed both in qualitative and quantitative terms, therefore ristomycin could be used to determine the so called ristocetin cofactor.

Actinoidin and vancomycin inhibited platelet agglutination induced by ristocetin or ristomycin in citrate-PRP or EDTA-PRP as well as in systems containing formaldehyde- treated platelets, but did not inhibit agglutination induced by bovine factor VIII.

All the four antibiotics caused plasma protein precipitation. Actinoidin was the least and vancomycin the most effective in this respect; ristocetin and ristomycin also possess this property, the effect of the latter is more considerable.

Actinoidin and vancomycin did not block the immediate increase in light absorbancy in aggregometer caused by the addition of ristocetin or ristomycin to fixed platelets at concentrations which totally inhibited platelet agglutination in the presence of protein cofactor. Inhibition of this »direct effect« of ristocetin and ristomycin was observed only at higher concentrations indicating that this effect may be unrelated to the agglutination.

According to our results with ristomycin derivatives the methylated carboxyl and the free phenolic hydroxyl groups may be of prime importance in the binding of ristomycin to the platelet membrane and/or to its protein cofactor. Similar data from other laboratories are confirmed and some new findings are offered.

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References

References
1 Baugh RR, Jacoby C, Brown JE, Hougie C. 1978; Effect of vancomycin on ristocetin and bovine PAF-induced agglutination of human platelets. Thrombosis Research 12: 511
2 Best GK, Best NH, Iurham NN. 1968; Chromatographic separation of the vancomycin complex. Antimicrobial Agents and Chemotherapy p 115
3 Bognar R, Makleit S, Sztaricskai R, Lomakina NN, Yurina MS. 1964; Synthesis of the aminoacids of the hydrolysate of actinoidins. Antibiotiki 9: 875
4 Bognar R, Sztaricskai R, Brazhnikova MG, Lomakina NN, Zenkova UA. 1974; a Structure investigation of aglycon of ristomycin A. Magyar Kémiai Folyoirat 80: 503 (in Hungarian)
4 Bognar R, Sztaricskai F, Munk M, Tamas J. 1974; b Structure and stereochemistry of ristosamine. Journal of Organic Chemistry 39: 2971
5 Brazhnikova MG, Lomakina NN, Lavrova MF, Tolstykh IV, Yurina MS, Kyueva LM. 1963; Isolation and properties of ristomycin. Antibiotiki 8: 392
6 Brazhnikova MG, Lomakina NN, Sztaricskai F, Puskas M, Makleit S, Bognar R. 1967; On the vancomycin type of antibiotics. Kemiai kozlemenyek 27: 143
7 Coller BS. 1978; The effects of ristocetin and von Willebrand factor on platelet electrophoretic mobility. Journal Clinical Investigation 78: 1168
8 Coller BS, Gralnick HR. 1977; Studies on the mechanisms of ristocetin-induced platelet agglutination. Journal of Clinical Investigation 60: 302
9 Evans RJ, Austen DE G. 1977; Assay of ristocetin cofactor using fixed platelets and a platelet counting technique. British Journal of Haematology 37: 389
10 Fehlner JR, Hutchinson RE J, Tarbell DS, Schenk JR. 1972; Structure of ristocetin A. Proceedings of the National Academy of Sciences USA 69: 2420
11 Fischer W, Germer WD. 1957; t)ber eine einfache Methode der direkten phasenoptischen Thrombozytenzahlung. R�ntgen- und Laboratorium-Praxis 10: 49
12 Floyd M, Burns W, Green D. 1977; On the interaction between ristocetin and ristocetin cofactor (RCOF). Thrombosis Research 10: 841
13 Harris TM, Fehlner JR, Raabe AB, Tarbell DS. 1975; Oxidative degradation of ristocetin A. Tetrahedron Letters p 2655
14 Harris CM, Kirby JJ, Harris TM. 1978; The biphenyl constituent of ristocetin A. Tetrahedron Letters p 705
15 Higgins HM, Harrison WH, Wild GM, Bungay HR, Mccormic MH. 1957; Vancomycin, a new antibiotic. VI. Purification and properties of vancomycin. Antibiotics Annual p 906
16 Howard MA, Firkin BG. 1971; Ristocetin - a new tool in the investigation of platelet aggregation. Thrombosis and Diathesis Haemorrhagica 26: 362
17 Johnson AW, Smith RM. 1972; a Vancomycin. Hydrolysis and oxidation studies Journal of Antibiotics 25: 292
18 Johnson AW, Smith RM, Guthrie RD. 1972; b Vancosamine: the structure and configuration of a novel amino-sugar from vancomycin. J. C.S. Perkin Transaction 1 p 2153
191 Katruha GS, Terentev PB, Gerstein ES. 1978; a On the structure of diamino-dicarboxilic acids of the antibiotic ristomycin A. Himia Prirodniih Soedineniej p 1941
20 Katruha GS, Silaev AB, Trifonova ZHP, Diarra B, Lomakina NN, Tokareva NL, Baratova LA, Belyanova LP. 1978; b Quantitativ amino acid composition and characteristics of amino groups of ristomycin and products of its partial acid hydrolysis. Antibiotiki 23: 38
21 Kattlove HE, Gomez MH. 1975; Studies on the mechanisms of ristocetin-induced platelet aggregation. Blood 45: 91
22 Kirby EP. 1977; Factor VHI-associated platelet aggregation. Thrombosis and Haemostasis 38: 1054
23 Kirby EP, Mills DC B. 1975; The interaction of bovine factor VIII with human platelets. Journal of Clinical Investigation 56: 491
24 Kirby EP, Tang SM. 1977; The binding of bovine factor VIII to human platelets. Thrombosis and Haemostasis 38: 126 (abstract)
25 Lavrova MF, Lomakina NN, Babenko NN, Brazhnikova MG. 1964; Separation of ristomycin variants. Antibiotiki 9: 885
26 Lomakina NN, Spiridonova IA, Sheinker JN, Vlasova TF. 1973; Structures of aminosugars isolated from actinoidine. Himia Prirodniih Soedineniej 101
27 Lomakina NN, Zenkova VA, Bognar R, Sztaricskai F, Sheinker JUN, Turchin KF. 1968; Structure of amino acids from ristomycin. Amino-acid A. Antibiotiki 13: 675
28 Lomakina NN, Zenkova VA, Yurina MS. 1969; Absolute configurations of amino-acids of actinoidins. Himia Prirodniih Soedineniej 43
29 Lomakina NN, Yurina MS, Brazhnikova MG. 1964; Isolation and analysis of actinoidin hydrolizate amino-acid components. Antibiotiki 9: 880
30 Lomakina NN, Yurina MS, Lavrova MF, Brazhnikova MG. 1961; Actinoidin and its separation into biologically active variants. Antibiotiki 6: 609
31 Lomakina NN, Yurina MS, Sheinker JUN, Turchin KF. 1972; Structure of amino-acids in ristomycin. Amino-acids B and C. Antibiotiki 17: 488
32 Lombart C, Okumura T, Jamieson GA. 1974; Isolation of a surface glycoprotein of human platelets. FEBS letters 41: 30
33 Lukacs G, Smith RM. 1972. Resonance magnétique nucléaire du ¹³C des produits naturels et apparents. Une application structurale: la vancosamine.. Bulletin de la Société Chimique de France; p 3995
34 Marshall FJ. 1965; Structure studies on vancomycin. Journal of Medicinal Chemistry 8: 18
35 Mccormick MH, Stark WM, Pittinger GE, Pittinger RC, Mcguire JM. 1955; Vancomycin, a new antibiotic, I. Chemical and biological properties. Antimicrobial Annual p 606
36 Moake JL, Cimo PL, Peterson DM, Roper P, Natelson EA. 1977; Inhibition of ristocetin-induced platelet agglutination by vancomycin. Blood 50: 397
37 Neszmelyi F, Sztaricskai F, Liptak A, Bognar R. 1978 Structural investigation of the antibiotic ristomycin A. ¹³C-NMR spectral analysis of the interglycosidic linkages of the heterotet- rasaccharide side-chain. Journal of Antibiotics (in press)
38 Nieto M, Perkins HR. 1971; The specificity of combinations between ristocetin and peptides related to bacterial cell wall mucopeptide precursors. Biochemical Journal 124: 845
39 Okumura T, Jamieson GA. 1976; a Platelet glycocalicin: a single receptor for platelet aggregation induced by thrombin or ristocetin. Thrombosis Research 8: 701
40 Okumura T, Jamieson GA. 1976; b Platelet glycocalicin. I. Orientation of glycoproteins of the human platelet surface. Journal of Biological Chemistry 251: 5944
41 Philip JE, Schenck JR, Hargie MP. 1956; Ristocetin A and B. Two new antibiotics. Isolation and properties Antibiotics Annual p 699
42 Rak K, Boda Z, Sztaricskai F. 1978. Comparative study of ristocetin and ristomycin induced platelet aggregation. Abstracts of XVIIth Congress of The International Society of Hematology. Paris: 1978. Vol.1 p 365
43 Roberts PJ, Kennard O, Smith KE, Williams DH. 1973; Concerning the molecular weight and structure of the antibiotic vancomycin. J. C. S. Chemical Communications p 772
44 Scheldrick GM, Jones PG, Kennard O, Williams DH, Smith GA. 1978; Structure of vancomycin and its complex with acetyl-D-alanyl-D-alanine. Nature 271: 223
45 Smith GA, Smith KA, Williams DH. 1975; Structural studies on the antibiotic vancomycin: Evidence for the presence of modified phenylglycine and -hydroxy-tyrosine units. J. C.S. Perkin Transactions 1 p 2108
46 Smith RM, Johnson AW, Guthrie RD. 1972; Vancosamine. A novel branched chain amino-sugar from the antibiotic vancomycin J. C.S. Chemical Communications p 361
47 Solum NO. 1968; Aggregation of human platelets by bovine platelet fibrinogen. Scandinavian Journal of Haematology 5: 474
48 Solum NO, Hagen I. 1978 Membrane receptors in platelet adhesion and secretion. Bibliotheca haematologica. In press
49 Solum NO, Hagen I, Gjemdal T. 1977; a Platelet membrane glycoproteins and the interaction between bovine factor VIII related protein and human platelets. Thrombosis and Haemostasis 38: 914
50 Solum NO, Hagen I, Peterka M. 1977; b Human platelet glycoproteins. Further evidence that the “GPI band” from whole platelet contains three different polypeptides one of which may be involved in the interaction between platelets and factor VIII. Thrombosis Research 10: 71
51 Solum NO, Peterka M. 1977. C Interactions of platelets with factor VHI-related protein. Platelets and Thrombosis, Procedings of the Serono Symposia, 10, 21. Academic Press;
52 Sorin VA, Judincev SD, Kuhrat JA, Goldberg LE, Peuzner NS, Brazhnikova MG, Lomakina NN, OpapüSeva EF. 1957; A new antibiotic, the actinoidine. Antibiotiki 2: 44
53 Sztaricskai F, Bognar R, Puskas MM. 1975. Structural investigation of the antibiotic ristomycin A. Chemical structure of the oligosaccharide part of ristomycin A. Acta Chimica Academiae Scientiarum Hungaricae; 84 75
53 Sztaricskai F, Liptak A, Pelyvas IF, Bognar R. 1978; a Structural investigation of the antibiotics ristomycin A. Synthesis of ristobiose and ristotriose Journal of Antibiotics 29: 626
54 Sztaricskai F, Lomakina NN, Spiridinova IA, Yurina MS, Puskas M. 1967; Quantitative determination of carbohydrates in actinoidines A and B. Antibiotiki 12: 126
55 Sztaricskai F, Pelyvas I, Bognar R. 1978 b Structure investigation of the carbohydrate moiety of ristomycin A. IXth International Symposium on the Carbohydrate Chemistry London. 1978
56 Östvold AC, Lindberg ME, Solum NO, Holmsen H. 1975; Adenine nucleotide metabolism during the aggregation of human platelets by bovine factor VIII. Haemostasis 4: 3
57 Zucker MB, Sook-Ja Kim, Mcpherson J, Grant RA. 1977; Binding of factor VIII to platelets in the presence of ristocetin. British Journal of Haematology 35: 535
58 Weiss HJ, Mayer LW, Rickles FR, Varma A, Rogers J. 1973; a Quantitative assay of a plasma factor deficient in von Willebrand‘s disease that in necessary for platelet aggregation. Journal of Clinical Investigation 52: 2708
59 Weiss HJ, Rogers J, Brand H. 1973; b Defective ristocetin induced platelet aggregation in von Willebrand’s disease and its correction by factor VIII. Journal of Clinical Investigation 52: 2697
60 Williams DH, Kalman JR. 1976; The ¹³C magnetic resonance spectrum of the antibiotic vancomycin. Tetrahedron Letters p 4829