Download PDF
Thromb Haemost 1979; 42(04): 1217-1229
DOI: 10.1055/s-0038-1657017
Original Article
Schattauer GmbH Stuttgart

Interaction of Bovine Platelets with Bovine Glomerular Basement Membrane[*]

J Laurence Davis
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Donna K F Chandler
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Patrick Riquetti
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Stearns Rogers
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Katherine M Kocan
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Charlotte L Ownby
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Billy G Hudson
**   The Department of Biochemistry and Molecular Biology, Kansas University Medical Center, Kansas City, Kansas, USA
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
,
Kermit L Carraway
The Departments of Biochemistry and Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
› Author Affiliations
Further Information

Publication History

Received 22 April 1978

Accepted 26 March 1979

Publication Date:
23 August 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

The interaction of bovine platelets with bovine glomerular basement membrane has been studied by aggregometry, transmission and scanning electron microscopy and measurement of [3H] serotonin release. In the absence of added calcium platelets adhere to basement membrane but fail to undergo the release reaction or aggregation. In the presence of 0.2-0.5 mM calcium release of serotonin and complete aggregation of the platelets are observed when sufficient basement membrane is present. Platelets were strongly adhered to the basement membrane surface, the platelet surface in the aggregates closely following the surface of the basement membrane. Platelet morphology in aggregates with basement membrane closely resembled that of platelets from collagen-induced aggregates. Basement membrane differed from collagen in its requirement for calcium for the aggregation and release reactions. In addition purified basement membrane was 1.5-3 fold less active on a weight basis than bovine tendon collagen in promoting aggregation.

*Journal article J-3383 of the Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma. This research was conducted in cooperation with the USDA, Agricultural Research Service, Southern Region.


 

  • References

  • 1 Weiss HJ. 1975; Platelet Physiology and abnormalities of platelet function. N. Eng. J. Med 293: 531-541
    CrossrefPubMedGoogle Scholar
  • 2 Smith JB, MacFarlane DE. 1974. The Prostaglandins II. Ramwell PW. editor Plenum Press; New York: 293
    Google Scholar
  • 3 Chesney CMc I, Harper E, Colman RW. 1972; Critical role for the carbohydrate side chain of collagen in platelet aggregation. J. Clin. Invest 51: 2693-2701
    CrossrefPubMedGoogle Scholar
  • 4 Puett D, Wasserman BK, Ford JD, Cunningham LW. 1973; Collagen-mediated platelet aggregation. Effects of collagen modification involving the protein and carbohydrate moieties J. Clin. Invest 52: 2495-2506
    CrossrefPubMedGoogle Scholar
  • 5 Wilner GD, Nossel HL, Leroy EC. 1968; Aggregation of platelets by collagen. J. Clin. Invest 47: 2616-2625
    CrossrefPubMedGoogle Scholar
  • 6 Nossel HL, Wilner GD, Leroy EC. 1969; Importance of polar groups for initiating blood coagulation and aggregating platelets. Nature 221: 75-76
    CrossrefPubMedGoogle Scholar
  • 7 Wilner GD, Nossel HL, Procupez TL. 1971; Aggregation of platelets by collagen: Polar active sites of insoluble human collagen. Am. J. Physiol 220: 1074-1079
    PubMedGoogle Scholar
  • 8 Jamieson GA, Urban CL, Barber AJ. 1971; Enzymatic basis for platelet: collagen adhesion as the primary step in hemostasis. Nature New Biol 234: 5-7
    PubMedGoogle Scholar
  • 9 Barber AJ, Jamieson GA. 1971; Characterization of membrane-bound collagen galactosyltransferase of human blood platelets. Biochim. Biophys. Acta 252: 546-552
    CrossrefPubMedGoogle Scholar
  • 10 Bosman HB. 1971; Platelet adhesiveness and aggregation: the collagen: glycosyl, polypeptide: N-acetylgalactosaminyl and glycoprotein galactosyl transferases in human platelets. Biochem. Biophys. Res Commun. 43: 1118-1124
    CrossrefPubMedGoogle Scholar
  • 11 Jaffe R, Deykin D. 1974; Evidence for a structural requirement for the aggregation of platelets by collagen. J. Clin. Invest. 53: 875-883
    CrossrefPubMedGoogle Scholar
  • 12 Muggli R, Baumgartner HR. 1973; Collagen induced platelet aggregation: requirement for tropocollagen multimeres. Thromb. Res 3: 715-728
    CrossrefPubMedGoogle Scholar
  • 13 Brass LF, Bensusan HB. 1974; The role of collagen quaternary structure in the platelet: collagen interaction. J. Clin. Invest. 54: 1480-1487
    CrossrefPubMedGoogle Scholar
  • 14 Balleisen L, Marx R, Kuhn K. 1976; Platelet-collagen interaction. The influence of native and modified collagen (Type I) on the aggregation of human platelets Haemostasis 5: 155-164
    PubMedGoogle Scholar
  • 15 Chiang TM, Beachey EH, Kang AH. 1975; Interaction of a chick skin collagen fragment (αl-CB5) with human platelets. J. Biol. Chem. 250: 6916-6922
    PubMedGoogle Scholar
  • 16 Ts’Ao CH. 1971; In vitro platelet reaction with isolated glomerular basement membrane. Thromb. Diath. Haemorrh. 25: 507-515
    PubMedGoogle Scholar
  • 17 Hugues J, Mahieu P. 1970; Platelet aggregation induced by basement membrane. Thromb. Diath. Haemorrh. 24: 395-408
    PubMedGoogle Scholar
  • 18 Huang TW, Lagunoff D, Benditt EP. 1974; Nonaggregative adherence of platelets to basal lamina in vitro. Lab Invest 31: 156-160
    PubMedGoogle Scholar
  • 19 Wang CL, Miyata T, Schlear S, Weksler B, Rubin AL, Stenzel KH. 1975; Collagen and glomerular basement membrane effects on platelets. Trans. Amer. Soc. Artif. Int Organs 21: 422-426
    PubMedGoogle Scholar
  • 20 Freytag JW, Ohno M, Hudson BG. 1976; Bovine renal glomerular basement membrane. Assessment of proteolysis during isolation Biochem. Biophys, Res. Commun 72: 796-802
    CrossrefPubMedGoogle Scholar
  • 21 Freytag JW, Dalrymple PN, Maguire MH, Stricklang DK, Carraway KL, Hudson BG. 1977; Bovine renal glomerular basement membrane. Identification of a plateletaggregating component Fed. Proc. 36: 679 (Abs)
    PubMedGoogle Scholar
  • 22 Freytag JW, Dalrymple PN, Maguire MH, Strickland DK, Carraway KL, Hudson BG. 1978; Glomerular basement membrane: Studies on its structure and interaction with platelets. J. Biol. Chem. 253: 9069-9074
    PubMedGoogle Scholar
  • 23 Holmsen H, Day JH, Storm E. 1969; Adenine Nucleotide Metabolism of Blood Platelets. VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction. Biochim. Biophys. Acta 186: 254-266
    CrossrefPubMedGoogle Scholar
  • 24 Mattson JC, Borgerding PJ, Craft DL. 1977; Fixation of platelets for scanning and transmission electron microscopy. Stain Tech. 52: 151-158
    PubMedGoogle Scholar
  • 25 Born GV, Cross MJ. 1963; The aggregation of blood platelets. J. Physiol 168: 178-195
    CrossrefPubMedGoogle Scholar
  • 26 Crawford N, Castle AG. 1976. Contractile Systems in Nonmuscle Tissues. Perry SV, Margreth A, Adelstein RS. editors Elsevier/North-Holland Biomedical Press; Amsterdam: 117
    Google Scholar
  • 27 Massini P, Luscher EF. 1974; Some effects of ionophores for divalent cations on blood platelets. Comparison with the effects of thrombin Biochim, Biophys. Acta 372: 109-121
    CrossrefPubMedGoogle Scholar
  • 28 Gerrard JM, White JG, RAO GH R. 1974; Effects of the ionophore A23187 on blood platelets. Am. J. Pathol. 77: 151-166
    PubMedGoogle Scholar